Peripheral blood CD163(+) monocytes and soluble CD163 in dry and neovascular age-related macular degeneration

Narsis Daftarian, Souska Zandi, Golbarg Piryaie, Mahin Nikougoftar Zarif, Ehsan Ranaei Pirmardan, Muneo Yamaguchi, Qurban Behzadian Nejad, Hossein Hasanpour, Shahram Samiei, Isabel B. Pfister, Zahra Soheila Soheili, Shintaro Nakao, Aliaa Barakat, Justus G. Garweg, Hamid Ahmadieh, Ali Hafezi-Moghadam

Research output: Contribution to journalArticle

Abstract

Macrophages are the main infiltrating immune cells in choroidal neovascularization (CNV), a hallmark of the human wet, or neovascular age-related macular degeneration (AMD). Due to their plasticity and ability to adapt to the local microenvironment in a tissue-dependent manner, macrophages display polar functional phenotypes characterized by their cell surface markers and their cytokine profiles. We found accumulation of hemoglobin-scavenging cluster of differentiation 163 (CD163)(+) macrophages in laser-induced CNV lesions and higher expression of CD163(+) monocytes in the peripheral blood on day 7 post injury in mice. In comparison, CD80(+) macrophages did not differ with laser-injury in young or aged mice and did not significantly change in the peripheral blood of CNV mice. We examined the percentages of CD163(+), CD206(+), and CD80(+) monocytes in the peripheral blood of patients with wet AMD, patients with dry AMD, and in age-matched individuals without AMD as controls. Percentages of peripheral blood CD163(+) monocytes in both dry AMD (P <.001) and wet AMD (P <.05) were higher than in age-matched non-AMD controls, while there was no difference between the groups in the percentages of peripheral CD206(+) and CD80(+) monocytes. Further, serum level of soluble CD163 (sCD163) was elevated only in patients with wet AMD (P <.05). An examination of 40 cytokine levels across the study groups revealed that anti-VEGF treated patients with wet AMD, who showed no exudative signs on the day of blood drawing had a cytokine profile that was similar to that of non-AMD individuals. These results indicate that CD163 could be further evaluated for its potential as a useful marker of disease activity in patients with neovascular AMD. Future studies will address the origin and potential mechanistic role of CD163(+) macrophages in wet AMD pathologies of angiogenesis and leakage of blood components.

Original languageEnglish
Pages (from-to)8001-8011
Number of pages11
JournalFASEB Journal
Volume34
Issue number6
DOIs
Publication statusPublished - Jun 1 2020

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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    Daftarian, N., Zandi, S., Piryaie, G., Nikougoftar Zarif, M., Ranaei Pirmardan, E., Yamaguchi, M., Behzadian Nejad, Q., Hasanpour, H., Samiei, S., Pfister, I. B., Soheili, Z. S., Nakao, S., Barakat, A., Garweg, J. G., Ahmadieh, H., & Hafezi-Moghadam, A. (2020). Peripheral blood CD163(+) monocytes and soluble CD163 in dry and neovascular age-related macular degeneration. FASEB Journal, 34(6), 8001-8011. https://doi.org/10.1096/fj.201901902RR