Peripheral blood circulating immature cell counts predict CD34+ cell yields in G-CSF-induced PBPC mobilization in healthy donors

Teruhiko Kozuka, Kazuma Ikeda, Takanori Teshima, Chikamasa Yoshida, Katsuji Shinagawa, Kensuke Kojima, Keitaro Matsuo, Akihiro Bessho, Kazutaka Sunami, Yasushi Hiramatsu, Yoshinobu Maeda, Toshio Noguchi, Kazuhiko Yamamoto, Nobuharu Fujii, Toshi Imai, Kinuyo Kaneda Kusumoto, Kozo Masuda, Katsuto Takenaka, Fumihiko Ishimaru, Kenji NiiyaNorio Koide, Mitsune Tanimoto, Mine Harada

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

BACKGROUND: It has been previously reported that the number of circulating immature cells (CIC) in peripheral blood (PB) estimates the number of CD34+ cells collected in G-CSF plus chemotherapy-induced PBPC mobilization. The correlation of CIC counts in PB with CD34+ cell yield and its usefulness was evaluated in G-CSF-induced PBPC mobilization for healthy donors. STUDY DESIGN AND METHODS: CIC counts in PB and CD34+ cell counts in the apheresis product from 122 collections were assessed, and the relationship between these two variables was evaluated with the Pearson rank correlation analysis, the chi-squared test, and the U-test. RESULTS: CIC counts were correlated weakly with the number of CD34+ cells per L of blood processed in the apheresis product (Pearson rank correlation analysis; r = 0.357, p < 0.0001). When a level of 1.7 x 109 CICs per L was selected as a cutoff value, the sensitivity and specificity for collecting more than 20 × 106 CD34+ cells per L of blood processed were 63.6 and 77.5 percent, respectively. CONCLUSION: The present study suggests that the number of CICs in PB may estimate the number of CD34+ cells collected. The data indicate that CIC counts above 1.7 × 109 per L can be used as a good predictor for PBPC collections containing more than 20 × 106 CD34+ cells per L of blood processed in a single apheresis procedure.

Original languageEnglish
Pages (from-to)526-532
Number of pages7
JournalTransfusion
Volume44
Issue number4
DOIs
Publication statusPublished - Apr 1 2004

Fingerprint

Granulocyte Colony-Stimulating Factor
Cell Count
Blood Component Removal
Blood Cell Count
Drug Therapy
Sensitivity and Specificity

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Hematology

Cite this

Peripheral blood circulating immature cell counts predict CD34+ cell yields in G-CSF-induced PBPC mobilization in healthy donors. / Kozuka, Teruhiko; Ikeda, Kazuma; Teshima, Takanori; Yoshida, Chikamasa; Shinagawa, Katsuji; Kojima, Kensuke; Matsuo, Keitaro; Bessho, Akihiro; Sunami, Kazutaka; Hiramatsu, Yasushi; Maeda, Yoshinobu; Noguchi, Toshio; Yamamoto, Kazuhiko; Fujii, Nobuharu; Imai, Toshi; Kusumoto, Kinuyo Kaneda; Masuda, Kozo; Takenaka, Katsuto; Ishimaru, Fumihiko; Niiya, Kenji; Koide, Norio; Tanimoto, Mitsune; Harada, Mine.

In: Transfusion, Vol. 44, No. 4, 01.04.2004, p. 526-532.

Research output: Contribution to journalArticle

Kozuka, T, Ikeda, K, Teshima, T, Yoshida, C, Shinagawa, K, Kojima, K, Matsuo, K, Bessho, A, Sunami, K, Hiramatsu, Y, Maeda, Y, Noguchi, T, Yamamoto, K, Fujii, N, Imai, T, Kusumoto, KK, Masuda, K, Takenaka, K, Ishimaru, F, Niiya, K, Koide, N, Tanimoto, M & Harada, M 2004, 'Peripheral blood circulating immature cell counts predict CD34+ cell yields in G-CSF-induced PBPC mobilization in healthy donors', Transfusion, vol. 44, no. 4, pp. 526-532. https://doi.org/10.1111/j.1537-2995.2004.03078.x
Kozuka, Teruhiko ; Ikeda, Kazuma ; Teshima, Takanori ; Yoshida, Chikamasa ; Shinagawa, Katsuji ; Kojima, Kensuke ; Matsuo, Keitaro ; Bessho, Akihiro ; Sunami, Kazutaka ; Hiramatsu, Yasushi ; Maeda, Yoshinobu ; Noguchi, Toshio ; Yamamoto, Kazuhiko ; Fujii, Nobuharu ; Imai, Toshi ; Kusumoto, Kinuyo Kaneda ; Masuda, Kozo ; Takenaka, Katsuto ; Ishimaru, Fumihiko ; Niiya, Kenji ; Koide, Norio ; Tanimoto, Mitsune ; Harada, Mine. / Peripheral blood circulating immature cell counts predict CD34+ cell yields in G-CSF-induced PBPC mobilization in healthy donors. In: Transfusion. 2004 ; Vol. 44, No. 4. pp. 526-532.
@article{9e1a94da5d9140c5891c2e3458abaea2,
title = "Peripheral blood circulating immature cell counts predict CD34+ cell yields in G-CSF-induced PBPC mobilization in healthy donors",
abstract = "BACKGROUND: It has been previously reported that the number of circulating immature cells (CIC) in peripheral blood (PB) estimates the number of CD34+ cells collected in G-CSF plus chemotherapy-induced PBPC mobilization. The correlation of CIC counts in PB with CD34+ cell yield and its usefulness was evaluated in G-CSF-induced PBPC mobilization for healthy donors. STUDY DESIGN AND METHODS: CIC counts in PB and CD34+ cell counts in the apheresis product from 122 collections were assessed, and the relationship between these two variables was evaluated with the Pearson rank correlation analysis, the chi-squared test, and the U-test. RESULTS: CIC counts were correlated weakly with the number of CD34+ cells per L of blood processed in the apheresis product (Pearson rank correlation analysis; r = 0.357, p < 0.0001). When a level of 1.7 x 109 CICs per L was selected as a cutoff value, the sensitivity and specificity for collecting more than 20 × 106 CD34+ cells per L of blood processed were 63.6 and 77.5 percent, respectively. CONCLUSION: The present study suggests that the number of CICs in PB may estimate the number of CD34+ cells collected. The data indicate that CIC counts above 1.7 × 109 per L can be used as a good predictor for PBPC collections containing more than 20 × 106 CD34+ cells per L of blood processed in a single apheresis procedure.",
author = "Teruhiko Kozuka and Kazuma Ikeda and Takanori Teshima and Chikamasa Yoshida and Katsuji Shinagawa and Kensuke Kojima and Keitaro Matsuo and Akihiro Bessho and Kazutaka Sunami and Yasushi Hiramatsu and Yoshinobu Maeda and Toshio Noguchi and Kazuhiko Yamamoto and Nobuharu Fujii and Toshi Imai and Kusumoto, {Kinuyo Kaneda} and Kozo Masuda and Katsuto Takenaka and Fumihiko Ishimaru and Kenji Niiya and Norio Koide and Mitsune Tanimoto and Mine Harada",
year = "2004",
month = "4",
day = "1",
doi = "10.1111/j.1537-2995.2004.03078.x",
language = "English",
volume = "44",
pages = "526--532",
journal = "Transfusion",
issn = "0041-1132",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - Peripheral blood circulating immature cell counts predict CD34+ cell yields in G-CSF-induced PBPC mobilization in healthy donors

AU - Kozuka, Teruhiko

AU - Ikeda, Kazuma

AU - Teshima, Takanori

AU - Yoshida, Chikamasa

AU - Shinagawa, Katsuji

AU - Kojima, Kensuke

AU - Matsuo, Keitaro

AU - Bessho, Akihiro

AU - Sunami, Kazutaka

AU - Hiramatsu, Yasushi

AU - Maeda, Yoshinobu

AU - Noguchi, Toshio

AU - Yamamoto, Kazuhiko

AU - Fujii, Nobuharu

AU - Imai, Toshi

AU - Kusumoto, Kinuyo Kaneda

AU - Masuda, Kozo

AU - Takenaka, Katsuto

AU - Ishimaru, Fumihiko

AU - Niiya, Kenji

AU - Koide, Norio

AU - Tanimoto, Mitsune

AU - Harada, Mine

PY - 2004/4/1

Y1 - 2004/4/1

N2 - BACKGROUND: It has been previously reported that the number of circulating immature cells (CIC) in peripheral blood (PB) estimates the number of CD34+ cells collected in G-CSF plus chemotherapy-induced PBPC mobilization. The correlation of CIC counts in PB with CD34+ cell yield and its usefulness was evaluated in G-CSF-induced PBPC mobilization for healthy donors. STUDY DESIGN AND METHODS: CIC counts in PB and CD34+ cell counts in the apheresis product from 122 collections were assessed, and the relationship between these two variables was evaluated with the Pearson rank correlation analysis, the chi-squared test, and the U-test. RESULTS: CIC counts were correlated weakly with the number of CD34+ cells per L of blood processed in the apheresis product (Pearson rank correlation analysis; r = 0.357, p < 0.0001). When a level of 1.7 x 109 CICs per L was selected as a cutoff value, the sensitivity and specificity for collecting more than 20 × 106 CD34+ cells per L of blood processed were 63.6 and 77.5 percent, respectively. CONCLUSION: The present study suggests that the number of CICs in PB may estimate the number of CD34+ cells collected. The data indicate that CIC counts above 1.7 × 109 per L can be used as a good predictor for PBPC collections containing more than 20 × 106 CD34+ cells per L of blood processed in a single apheresis procedure.

AB - BACKGROUND: It has been previously reported that the number of circulating immature cells (CIC) in peripheral blood (PB) estimates the number of CD34+ cells collected in G-CSF plus chemotherapy-induced PBPC mobilization. The correlation of CIC counts in PB with CD34+ cell yield and its usefulness was evaluated in G-CSF-induced PBPC mobilization for healthy donors. STUDY DESIGN AND METHODS: CIC counts in PB and CD34+ cell counts in the apheresis product from 122 collections were assessed, and the relationship between these two variables was evaluated with the Pearson rank correlation analysis, the chi-squared test, and the U-test. RESULTS: CIC counts were correlated weakly with the number of CD34+ cells per L of blood processed in the apheresis product (Pearson rank correlation analysis; r = 0.357, p < 0.0001). When a level of 1.7 x 109 CICs per L was selected as a cutoff value, the sensitivity and specificity for collecting more than 20 × 106 CD34+ cells per L of blood processed were 63.6 and 77.5 percent, respectively. CONCLUSION: The present study suggests that the number of CICs in PB may estimate the number of CD34+ cells collected. The data indicate that CIC counts above 1.7 × 109 per L can be used as a good predictor for PBPC collections containing more than 20 × 106 CD34+ cells per L of blood processed in a single apheresis procedure.

UR - http://www.scopus.com/inward/record.url?scp=12144289435&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=12144289435&partnerID=8YFLogxK

U2 - 10.1111/j.1537-2995.2004.03078.x

DO - 10.1111/j.1537-2995.2004.03078.x

M3 - Article

C2 - 15043568

AN - SCOPUS:12144289435

VL - 44

SP - 526

EP - 532

JO - Transfusion

JF - Transfusion

SN - 0041-1132

IS - 4

ER -