Peripheral blood stem cell mobilization by granulocyte colony-stimulating factor alone and engraftment kinetics following autologous transplantation in children and adolescents with solid tumor

H. Watanabe, T. Watanabe, H. Suzuya, Yoshifumi Wakata, M. Kaneko, T. Onishi, Y. Okamoto, T. Abe, Y. Kawano, S. Kagami, Y. Takaue

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

In 56 pediatric and adolescent patients (median age 7 years, range 1-21) with various solid tumors, peripheral blood stem cells (PBSC) were mobilized with granulocyte colony-stimulating factor (G-CSF) alone, and the yields of PBSC and engraftment kinetics following autologous peripheral blood stem cell transplantation (PBSCT) were evaluated retrospectively. Granulocyte colony-stimulating factor (10 μg/kg) was injected subcutaneously for mobilization when patients showed no influence of previous chemotherapy, and administration was continued for 5 days. The peaks of CD34+ cells and colony-forming units-granulocyte/macrophage in the blood were observed on days 4 through 6 of G-CSF administration in all patients. Peripheral blood stem cell harvest was commenced on day 5 of G-CSF treatment. Compared to the results in patients mobilized by chemotherapy plus G-CSF (N = 18), the progenitor cell yields were lower in patients mobilized with G-CSF alone. However, there were no significant differences in WBC and ANC engraftment compared to the chemotherapy plus G-CSF mobilization group. Platelet recovery following autologous PBSCT was delayed in patients mobilized with G-CSF alone. The median time taken for ANC and platelet counts to reach 0.5 × 109 and 20 × 109/l was 12 days (range: 9-28) and 15 days (8-55), respectively, in all patients who received PBSC mobilized by G-CSF alone. In summary, mobilization with G-CSF alone can mobilize sufficient CD34+ cells for successful autografting and sustained hematological reconstitution in pediatric and adolescent patients with solid tumors, and even in heavily pre-treated patients.

Original languageEnglish
Pages (from-to)661-668
Number of pages8
JournalBone Marrow Transplantation
Volume37
Issue number7
DOIs
Publication statusPublished - Apr 1 2006

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Hematopoietic Stem Cell Mobilization
Autologous Transplantation
Granulocyte Colony-Stimulating Factor
Neoplasms
Peripheral Blood Stem Cell Transplantation
Drug Therapy
Peripheral Blood Stem Cells
Pediatrics
Granulocyte-Macrophage Progenitor Cells
Platelet Count
Stem Cells
Blood Platelets

All Science Journal Classification (ASJC) codes

  • Hematology
  • Transplantation

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Peripheral blood stem cell mobilization by granulocyte colony-stimulating factor alone and engraftment kinetics following autologous transplantation in children and adolescents with solid tumor. / Watanabe, H.; Watanabe, T.; Suzuya, H.; Wakata, Yoshifumi; Kaneko, M.; Onishi, T.; Okamoto, Y.; Abe, T.; Kawano, Y.; Kagami, S.; Takaue, Y.

In: Bone Marrow Transplantation, Vol. 37, No. 7, 01.04.2006, p. 661-668.

Research output: Contribution to journalArticle

Watanabe, H. ; Watanabe, T. ; Suzuya, H. ; Wakata, Yoshifumi ; Kaneko, M. ; Onishi, T. ; Okamoto, Y. ; Abe, T. ; Kawano, Y. ; Kagami, S. ; Takaue, Y. / Peripheral blood stem cell mobilization by granulocyte colony-stimulating factor alone and engraftment kinetics following autologous transplantation in children and adolescents with solid tumor. In: Bone Marrow Transplantation. 2006 ; Vol. 37, No. 7. pp. 661-668.
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AU - Wakata, Yoshifumi

AU - Kaneko, M.

AU - Onishi, T.

AU - Okamoto, Y.

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AU - Kagami, S.

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AB - In 56 pediatric and adolescent patients (median age 7 years, range 1-21) with various solid tumors, peripheral blood stem cells (PBSC) were mobilized with granulocyte colony-stimulating factor (G-CSF) alone, and the yields of PBSC and engraftment kinetics following autologous peripheral blood stem cell transplantation (PBSCT) were evaluated retrospectively. Granulocyte colony-stimulating factor (10 μg/kg) was injected subcutaneously for mobilization when patients showed no influence of previous chemotherapy, and administration was continued for 5 days. The peaks of CD34+ cells and colony-forming units-granulocyte/macrophage in the blood were observed on days 4 through 6 of G-CSF administration in all patients. Peripheral blood stem cell harvest was commenced on day 5 of G-CSF treatment. Compared to the results in patients mobilized by chemotherapy plus G-CSF (N = 18), the progenitor cell yields were lower in patients mobilized with G-CSF alone. However, there were no significant differences in WBC and ANC engraftment compared to the chemotherapy plus G-CSF mobilization group. Platelet recovery following autologous PBSCT was delayed in patients mobilized with G-CSF alone. The median time taken for ANC and platelet counts to reach 0.5 × 109 and 20 × 109/l was 12 days (range: 9-28) and 15 days (8-55), respectively, in all patients who received PBSC mobilized by G-CSF alone. In summary, mobilization with G-CSF alone can mobilize sufficient CD34+ cells for successful autografting and sustained hematological reconstitution in pediatric and adolescent patients with solid tumors, and even in heavily pre-treated patients.

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