Periploca sepium Bunge as a model plant for rubber biosynthesis study

Takeshi Bamba, Tomoki Sando, Asuka Miyabashira, Koichiro Gyokusen, Yoshihisa Nakazawa, Yinquan Su, Eiichiro Fukusaki, Akio Kobayashi

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Periploca sepium Bunge (Chinese silk vine) is a woody climbing vine belonging to the family Asclepiadaceae. It originally comes from Northwest China. Periploca resembles the Para-rubber tree, Hevea brasiliensis, regarding a similar body plan to produce a milky exudate containing rubber latex. The Periploca plant was assessed as a rubber-producing plant by rubber structure elucidation and its molecular weight distribution. A rubber fraction purified from the milky exudate was subjected to 1H NMR analysis, and a characteristic signal derived from cis-polyisoprene was observed. In addition, when the molecular weight distribution of rubber components in the exudate was measured (using size-exclusion chromatography), the number-average molecular weight (Mn), weight-average molecular weight (Mw), and polydispersity (Mw/Mn) were estimated to be Mn = 1.3 × 105, Mw = 4.1 × 10 5, and Mw/Mn = 3.1, respectively. Furthermore, the presence of polyisoprene, with Mn = 4.0 × 104, Mw = 7.6 × 10 4, and Mw/Mn = 2.5, was also confirmed in plantlets obtained from shoots as a result of tissue culture.

Original languageEnglish
Pages (from-to)579-582
Number of pages4
JournalZeitschrift fur Naturforschung - Section C Journal of Biosciences
Volume62
Issue number7-8
DOIs
Publication statusPublished - Jan 1 2007

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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