Permeability studies of nitroxyl spin probes through lipid membranes using L-band ESR spectrometer

A. Milton Franklin Benial, M. Kumara Dhas, Kazuhiro Ichikawa, Ken-Ichi Yamada, Fuminori Hyodo, A. Jawahar, Hideo Utsumi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Electron spin resonance studies were carried out for 2mM 14N labeled 2H enriched 3-methoxycarbonyl-2,2,5,5-tetramethyl- pyrrolidine-1-oxyl (MC-PROXYL) in pure water and 100, 200, 300, 400 mM concentrations of liposomal solution and 3-carboxy-2,2,5,5,-tetramethyl-1- pyrrolidinyloxy (carboxy-PROXYL) in pure water and 400 mM concentration of liposomal solution by using L-band ESR spectrometer. This study reveals that the permeability of MC-PROXYL in liposomal solution and impermeability of carboxy-PROXYL in 400 mM concentration of liposomal solution. This study illustrates that the ESR can be used to differentiate between the intra-and extra-membrane water by loading the liposome vesicles with a lipid permeable nitroxyl spin probe. The lipid membrane permeability of two different nitroxyl spin probes was demonstrated.

Original languageEnglish
Title of host publicationSolid State Physics - Proceedings of the 56th DAE Solid State Physics Symposium 2011
Pages185-186
Number of pages2
Edition1
DOIs
Publication statusPublished - Dec 1 2012
Event56th DAE Solid State Physics Symposium 2011 - Kattankulathur, Tamilnadu, India
Duration: Dec 19 2011Dec 23 2011

Publication series

NameAIP Conference Proceedings
Number1
Volume1447
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Other

Other56th DAE Solid State Physics Symposium 2011
CountryIndia
CityKattankulathur, Tamilnadu
Period12/19/1112/23/11

Fingerprint

ultrahigh frequencies
lipids
permeability
spectrometers
membranes
probes
water
electron paramagnetic resonance

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy(all)

Cite this

Franklin Benial, A. M., Dhas, M. K., Ichikawa, K., Yamada, K-I., Hyodo, F., Jawahar, A., & Utsumi, H. (2012). Permeability studies of nitroxyl spin probes through lipid membranes using L-band ESR spectrometer. In Solid State Physics - Proceedings of the 56th DAE Solid State Physics Symposium 2011 (1 ed., pp. 185-186). (AIP Conference Proceedings; Vol. 1447, No. 1). https://doi.org/10.1063/1.4709942

Permeability studies of nitroxyl spin probes through lipid membranes using L-band ESR spectrometer. / Franklin Benial, A. Milton; Dhas, M. Kumara; Ichikawa, Kazuhiro; Yamada, Ken-Ichi; Hyodo, Fuminori; Jawahar, A.; Utsumi, Hideo.

Solid State Physics - Proceedings of the 56th DAE Solid State Physics Symposium 2011. 1. ed. 2012. p. 185-186 (AIP Conference Proceedings; Vol. 1447, No. 1).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Franklin Benial, AM, Dhas, MK, Ichikawa, K, Yamada, K-I, Hyodo, F, Jawahar, A & Utsumi, H 2012, Permeability studies of nitroxyl spin probes through lipid membranes using L-band ESR spectrometer. in Solid State Physics - Proceedings of the 56th DAE Solid State Physics Symposium 2011. 1 edn, AIP Conference Proceedings, no. 1, vol. 1447, pp. 185-186, 56th DAE Solid State Physics Symposium 2011, Kattankulathur, Tamilnadu, India, 12/19/11. https://doi.org/10.1063/1.4709942
Franklin Benial AM, Dhas MK, Ichikawa K, Yamada K-I, Hyodo F, Jawahar A et al. Permeability studies of nitroxyl spin probes through lipid membranes using L-band ESR spectrometer. In Solid State Physics - Proceedings of the 56th DAE Solid State Physics Symposium 2011. 1 ed. 2012. p. 185-186. (AIP Conference Proceedings; 1). https://doi.org/10.1063/1.4709942
Franklin Benial, A. Milton ; Dhas, M. Kumara ; Ichikawa, Kazuhiro ; Yamada, Ken-Ichi ; Hyodo, Fuminori ; Jawahar, A. ; Utsumi, Hideo. / Permeability studies of nitroxyl spin probes through lipid membranes using L-band ESR spectrometer. Solid State Physics - Proceedings of the 56th DAE Solid State Physics Symposium 2011. 1. ed. 2012. pp. 185-186 (AIP Conference Proceedings; 1).
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AU - Hyodo, Fuminori

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AB - Electron spin resonance studies were carried out for 2mM 14N labeled 2H enriched 3-methoxycarbonyl-2,2,5,5-tetramethyl- pyrrolidine-1-oxyl (MC-PROXYL) in pure water and 100, 200, 300, 400 mM concentrations of liposomal solution and 3-carboxy-2,2,5,5,-tetramethyl-1- pyrrolidinyloxy (carboxy-PROXYL) in pure water and 400 mM concentration of liposomal solution by using L-band ESR spectrometer. This study reveals that the permeability of MC-PROXYL in liposomal solution and impermeability of carboxy-PROXYL in 400 mM concentration of liposomal solution. This study illustrates that the ESR can be used to differentiate between the intra-and extra-membrane water by loading the liposome vesicles with a lipid permeable nitroxyl spin probe. The lipid membrane permeability of two different nitroxyl spin probes was demonstrated.

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