Permeability studies of nitroxyl spin probes through lipid membranes using L-band ESR spectrometer

A. Milton Franklin Benial, M. Kumara Dhas, Kazuhiro Ichikawa, Ken-Ichi Yamada, Fuminori Hyodo, A. Jawahar, Hideo Utsumi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Electron spin resonance studies were carried out for 2mM 14N labeled 2H enriched 3-methoxycarbonyl-2,2,5,5-tetramethyl- pyrrolidine-1-oxyl (MC-PROXYL) in pure water and 100, 200, 300, 400 mM concentrations of liposomal solution and 3-carboxy-2,2,5,5,-tetramethyl-1- pyrrolidinyloxy (carboxy-PROXYL) in pure water and 400 mM concentration of liposomal solution by using L-band ESR spectrometer. This study reveals that the permeability of MC-PROXYL in liposomal solution and impermeability of carboxy-PROXYL in 400 mM concentration of liposomal solution. This study illustrates that the ESR can be used to differentiate between the intra-and extra-membrane water by loading the liposome vesicles with a lipid permeable nitroxyl spin probe. The lipid membrane permeability of two different nitroxyl spin probes was demonstrated.

Original languageEnglish
Title of host publicationSolid State Physics - Proceedings of the 56th DAE Solid State Physics Symposium 2011
Pages185-186
Number of pages2
Edition1
DOIs
Publication statusPublished - Dec 1 2012
Event56th DAE Solid State Physics Symposium 2011 - Kattankulathur, Tamilnadu, India
Duration: Dec 19 2011Dec 23 2011

Publication series

NameAIP Conference Proceedings
Number1
Volume1447
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Other

Other56th DAE Solid State Physics Symposium 2011
Country/TerritoryIndia
CityKattankulathur, Tamilnadu
Period12/19/1112/23/11

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy(all)

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