TY - GEN
T1 - Permeability studies of nitroxyl spin probes through lipid membranes using L-band ESR spectrometer
AU - Franklin Benial, A. Milton
AU - Dhas, M. Kumara
AU - Ichikawa, Kazuhiro
AU - Yamada, Ken-Ichi
AU - Hyodo, Fuminori
AU - Jawahar, A.
AU - Utsumi, Hideo
PY - 2012/12/1
Y1 - 2012/12/1
N2 - Electron spin resonance studies were carried out for 2mM 14N labeled 2H enriched 3-methoxycarbonyl-2,2,5,5-tetramethyl- pyrrolidine-1-oxyl (MC-PROXYL) in pure water and 100, 200, 300, 400 mM concentrations of liposomal solution and 3-carboxy-2,2,5,5,-tetramethyl-1- pyrrolidinyloxy (carboxy-PROXYL) in pure water and 400 mM concentration of liposomal solution by using L-band ESR spectrometer. This study reveals that the permeability of MC-PROXYL in liposomal solution and impermeability of carboxy-PROXYL in 400 mM concentration of liposomal solution. This study illustrates that the ESR can be used to differentiate between the intra-and extra-membrane water by loading the liposome vesicles with a lipid permeable nitroxyl spin probe. The lipid membrane permeability of two different nitroxyl spin probes was demonstrated.
AB - Electron spin resonance studies were carried out for 2mM 14N labeled 2H enriched 3-methoxycarbonyl-2,2,5,5-tetramethyl- pyrrolidine-1-oxyl (MC-PROXYL) in pure water and 100, 200, 300, 400 mM concentrations of liposomal solution and 3-carboxy-2,2,5,5,-tetramethyl-1- pyrrolidinyloxy (carboxy-PROXYL) in pure water and 400 mM concentration of liposomal solution by using L-band ESR spectrometer. This study reveals that the permeability of MC-PROXYL in liposomal solution and impermeability of carboxy-PROXYL in 400 mM concentration of liposomal solution. This study illustrates that the ESR can be used to differentiate between the intra-and extra-membrane water by loading the liposome vesicles with a lipid permeable nitroxyl spin probe. The lipid membrane permeability of two different nitroxyl spin probes was demonstrated.
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U2 - 10.1063/1.4709942
DO - 10.1063/1.4709942
M3 - Conference contribution
AN - SCOPUS:84876814985
SN - 9780735410442
T3 - AIP Conference Proceedings
SP - 185
EP - 186
BT - Solid State Physics - Proceedings of the 56th DAE Solid State Physics Symposium 2011
T2 - 56th DAE Solid State Physics Symposium 2011
Y2 - 19 December 2011 through 23 December 2011
ER -