Permeability studies of nitroxyl spin probes through lipid membranes using L-band ESR spectrometer

A. Milton Franklin Benial; M. Kumara Dhas; Kazuhiro Ichikawa; Ken-Ichi Yamada; Fuminori Hyodo; A. Jawahar; Hideo Utsumi

doi:10.1063/1.4709942

Permeability studies of nitroxyl spin probes through lipid membranes using L-band ESR spectrometer

A. Milton Franklin Benial, M. Kumara Dhas, Kazuhiro Ichikawa, Ken-Ichi Yamada, Fuminori Hyodo, A. Jawahar, Hideo Utsumi

Molecular Bioinformatics

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Electron spin resonance studies were carried out for 2mM ¹⁴N labeled ²H enriched 3-methoxycarbonyl-2,2,5,5-tetramethyl- pyrrolidine-1-oxyl (MC-PROXYL) in pure water and 100, 200, 300, 400 mM concentrations of liposomal solution and 3-carboxy-2,2,5,5,-tetramethyl-1- pyrrolidinyloxy (carboxy-PROXYL) in pure water and 400 mM concentration of liposomal solution by using L-band ESR spectrometer. This study reveals that the permeability of MC-PROXYL in liposomal solution and impermeability of carboxy-PROXYL in 400 mM concentration of liposomal solution. This study illustrates that the ESR can be used to differentiate between the intra-and extra-membrane water by loading the liposome vesicles with a lipid permeable nitroxyl spin probe. The lipid membrane permeability of two different nitroxyl spin probes was demonstrated.

Original language	English
Title of host publication	Solid State Physics - Proceedings of the 56th DAE Solid State Physics Symposium 2011
Pages	185-186
Number of pages	2
Edition	1
DOIs	https://doi.org/10.1063/1.4709942
Publication status	Published - Dec 1 2012
Event	56th DAE Solid State Physics Symposium 2011 - Kattankulathur, Tamilnadu, India Duration: Dec 19 2011 → Dec 23 2011

Publication series

Name	AIP Conference Proceedings
Number	1
Volume	1447
ISSN (Print)	0094-243X
ISSN (Electronic)	1551-7616

Other

Other	56th DAE Solid State Physics Symposium 2011
Country/Territory	India
City	Kattankulathur, Tamilnadu
Period	12/19/11 → 12/23/11

All Science Journal Classification (ASJC) codes

Physics and Astronomy(all)

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10.1063/1.4709942

Cite this

Franklin Benial, A. M., Dhas, M. K., Ichikawa, K., Yamada, K-I., Hyodo, F., Jawahar, A., & Utsumi, H. (2012). Permeability studies of nitroxyl spin probes through lipid membranes using L-band ESR spectrometer. In Solid State Physics - Proceedings of the 56th DAE Solid State Physics Symposium 2011 (1 ed., pp. 185-186). (AIP Conference Proceedings; Vol. 1447, No. 1). https://doi.org/10.1063/1.4709942

Permeability studies of nitroxyl spin probes through lipid membranes using L-band ESR spectrometer. / Franklin Benial, A. Milton; Dhas, M. Kumara; Ichikawa, Kazuhiro et al.

Solid State Physics - Proceedings of the 56th DAE Solid State Physics Symposium 2011. 1. ed. 2012. p. 185-186 (AIP Conference Proceedings; Vol. 1447, No. 1).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Franklin Benial, AM, Dhas, MK, Ichikawa, K, Yamada, K-I, Hyodo, F, Jawahar, A & Utsumi, H 2012, Permeability studies of nitroxyl spin probes through lipid membranes using L-band ESR spectrometer. in Solid State Physics - Proceedings of the 56th DAE Solid State Physics Symposium 2011. 1 edn, AIP Conference Proceedings, no. 1, vol. 1447, pp. 185-186, 56th DAE Solid State Physics Symposium 2011, Kattankulathur, Tamilnadu, India, 12/19/11. https://doi.org/10.1063/1.4709942

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abstract = "Electron spin resonance studies were carried out for 2mM 14N labeled 2H enriched 3-methoxycarbonyl-2,2,5,5-tetramethyl- pyrrolidine-1-oxyl (MC-PROXYL) in pure water and 100, 200, 300, 400 mM concentrations of liposomal solution and 3-carboxy-2,2,5,5,-tetramethyl-1- pyrrolidinyloxy (carboxy-PROXYL) in pure water and 400 mM concentration of liposomal solution by using L-band ESR spectrometer. This study reveals that the permeability of MC-PROXYL in liposomal solution and impermeability of carboxy-PROXYL in 400 mM concentration of liposomal solution. This study illustrates that the ESR can be used to differentiate between the intra-and extra-membrane water by loading the liposome vesicles with a lipid permeable nitroxyl spin probe. The lipid membrane permeability of two different nitroxyl spin probes was demonstrated.",

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AU - Franklin Benial, A. Milton

AU - Dhas, M. Kumara

AU - Ichikawa, Kazuhiro

AU - Yamada, Ken-Ichi

AU - Hyodo, Fuminori

AU - Jawahar, A.

AU - Utsumi, Hideo

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N2 - Electron spin resonance studies were carried out for 2mM 14N labeled 2H enriched 3-methoxycarbonyl-2,2,5,5-tetramethyl- pyrrolidine-1-oxyl (MC-PROXYL) in pure water and 100, 200, 300, 400 mM concentrations of liposomal solution and 3-carboxy-2,2,5,5,-tetramethyl-1- pyrrolidinyloxy (carboxy-PROXYL) in pure water and 400 mM concentration of liposomal solution by using L-band ESR spectrometer. This study reveals that the permeability of MC-PROXYL in liposomal solution and impermeability of carboxy-PROXYL in 400 mM concentration of liposomal solution. This study illustrates that the ESR can be used to differentiate between the intra-and extra-membrane water by loading the liposome vesicles with a lipid permeable nitroxyl spin probe. The lipid membrane permeability of two different nitroxyl spin probes was demonstrated.

AB - Electron spin resonance studies were carried out for 2mM 14N labeled 2H enriched 3-methoxycarbonyl-2,2,5,5-tetramethyl- pyrrolidine-1-oxyl (MC-PROXYL) in pure water and 100, 200, 300, 400 mM concentrations of liposomal solution and 3-carboxy-2,2,5,5,-tetramethyl-1- pyrrolidinyloxy (carboxy-PROXYL) in pure water and 400 mM concentration of liposomal solution by using L-band ESR spectrometer. This study reveals that the permeability of MC-PROXYL in liposomal solution and impermeability of carboxy-PROXYL in 400 mM concentration of liposomal solution. This study illustrates that the ESR can be used to differentiate between the intra-and extra-membrane water by loading the liposome vesicles with a lipid permeable nitroxyl spin probe. The lipid membrane permeability of two different nitroxyl spin probes was demonstrated.

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Permeability studies of nitroxyl spin probes through lipid membranes using L-band ESR spectrometer

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