Permeability studies of redox-sensitive nitroxyl radicals through bilayer lipid membranes

M. Kumara Dhas, A. Milton Franklin Benial, Kazuhiro Ichikawa, Ken Ichi Yamada, Fuminori Hyodo, A. Jawahar, Hideo Utsumi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Electron spin resonance (ESR) studies were carried out for 2mM 14N-labeled deutrated 3-methoxycarbonyl-2,2,5,5-tetramethyl- pyrrolidine-1-oxyl (MC-PROXYL) and 3-carboxy-2,2,5,5,-tetramethyl-1- pyrrolidinyloxy (carboxy-PROXYL) in pure water and various concentrations of liposomal solutions by using 300 MHz ESR spectrometer. The ESR parameters such as the line width, hyperfine coupling constant, g-factor, rotational correlation time, partition parameter and permeability were reported for the samples. The permeable and impermeable nature of nitroxyl spin probes was demonstrated. The increased rotational correlation time was observed for MC-PROXYL, which indicates the permeable and less mobile nature of the nitroxyl spin probe. The obtained partition parameter and permeability values are used to quantify the permeable nature of the nitroxyl spin probe. The peaks correspond to lipid phase were observed for MC-PROXYL in liposomal solution, but that peak was not observed for carboxy-PROXYL. These results indicate the permeable and the impermeable nature of nitroxyl spin probes.

Original languageEnglish
Title of host publicationSolid State Physics - Proceedings of the 57th DAE Solid State Physics Symposium 2012
Pages132-133
Number of pages2
DOIs
Publication statusPublished - Mar 15 2013
Event57th DAE Solid State Physics Symposium 2012 - Bombay, Mumbai, India
Duration: Dec 3 2012Dec 7 2012

Publication series

NameAIP Conference Proceedings
Volume1512
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Other

Other57th DAE Solid State Physics Symposium 2012
CountryIndia
CityBombay, Mumbai
Period12/3/1212/7/12

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy(all)

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    Dhas, M. K., Benial, A. M. F., Ichikawa, K., Yamada, K. I., Hyodo, F., Jawahar, A., & Utsumi, H. (2013). Permeability studies of redox-sensitive nitroxyl radicals through bilayer lipid membranes. In Solid State Physics - Proceedings of the 57th DAE Solid State Physics Symposium 2012 (pp. 132-133). (AIP Conference Proceedings; Vol. 1512). https://doi.org/10.1063/1.4790946