TY - JOUR
T1 - Permeability Studies of Redox-Sensitive Nitroxyl Spin Probes Through Lipid Membranes Using an L-Band ESR Spectrometer
AU - Milton Franklin Benial, A.
AU - Kumara Dhas, M.
AU - Ichikawa, Kazuhiro
AU - Yamada, Ken Ichi
AU - Hyodo, Fuminori
AU - Jawahar, A.
AU - Utsumi, Hideo
N1 - Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2013/4
Y1 - 2013/4
N2 - Electron spin resonance (ESR) studies were carried out for 2 mM 14N-labeled deuterated 3-methoxycarbonyl-2,2,5,5-tetramethyl-pyrrolidine-1-oxyl (MC-PROXYL) and 3-carboxy-2,2,5,5,-tetramethyl-pyrrolidin-1-oxyl (carboxy-PROXYL) in pure water and various concentrations of liposomal solution by using an L-band ESR spectrometer. The ESR parameters, such as the line width, hyperfine coupling constant, g-factor, rotational correlation time and partition parameter, were reported for the samples. The changes in the line width were observed for 14N-labeled deuterated MC-PROXYL and carboxy-PROXYL in liposomal solution. The hyperfine coupling constant was observed for both nitroxyl spin probes. The permeable and impermeable nature of nitroxyl radicals was demonstrated using the ESR L-band spectra. The rotational correlation time increases with increasing concentration of liposome. The partition parameter for 14N-labeled deuterated MC-PROXYL in liposomal solution increases with increasing concentration of liposome, which reveals that the nitroxyl spin probe permeates into lipid membrane. The lipid peaks were observed for 2 mM 14N-labeled deuterated MC-PROXYL in 200, 300 and 400 mM liposomal concentration. The lipid peaks were not observed for 14N-labeled deuterated carboxy-PROXYL. These results indicate the permeable and impermeable nature of 14N-labeled deuterated nitroxyl spin probe.
AB - Electron spin resonance (ESR) studies were carried out for 2 mM 14N-labeled deuterated 3-methoxycarbonyl-2,2,5,5-tetramethyl-pyrrolidine-1-oxyl (MC-PROXYL) and 3-carboxy-2,2,5,5,-tetramethyl-pyrrolidin-1-oxyl (carboxy-PROXYL) in pure water and various concentrations of liposomal solution by using an L-band ESR spectrometer. The ESR parameters, such as the line width, hyperfine coupling constant, g-factor, rotational correlation time and partition parameter, were reported for the samples. The changes in the line width were observed for 14N-labeled deuterated MC-PROXYL and carboxy-PROXYL in liposomal solution. The hyperfine coupling constant was observed for both nitroxyl spin probes. The permeable and impermeable nature of nitroxyl radicals was demonstrated using the ESR L-band spectra. The rotational correlation time increases with increasing concentration of liposome. The partition parameter for 14N-labeled deuterated MC-PROXYL in liposomal solution increases with increasing concentration of liposome, which reveals that the nitroxyl spin probe permeates into lipid membrane. The lipid peaks were observed for 2 mM 14N-labeled deuterated MC-PROXYL in 200, 300 and 400 mM liposomal concentration. The lipid peaks were not observed for 14N-labeled deuterated carboxy-PROXYL. These results indicate the permeable and impermeable nature of 14N-labeled deuterated nitroxyl spin probe.
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U2 - 10.1007/s00723-012-0383-0
DO - 10.1007/s00723-012-0383-0
M3 - Article
AN - SCOPUS:84874691154
VL - 44
SP - 439
EP - 447
JO - Applied Magnetic Resonance
JF - Applied Magnetic Resonance
SN - 0937-9347
IS - 4
ER -