Peroxisomal membrane and matrix protein import using a semi-intact mammalian cell system

Kanji Okumoto, Masanori Honsho, Yuqiong Liu, Yukio Fujiki

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Peroxisomes are essential intracellular organelles that catalyze a number of essential metabolic pathways including β-oxidation of very long chain fatty acids, synthesis of plasmalogen, bile acids, and generation and degradation of hydrogen peroxide. These peroxisomal functions are accomplished by strictly and spatiotemporally regulated compartmentalization of the enzymes catalyzing these reactions. Defects in peroxisomal protein import result in inherited peroxisome biogenesis disorders in humans. Peroxisomal matrix and membrane proteins are synthesized on free ribosomes and transported to peroxisomes in a manner dependent on their specific targeting signals and their receptors. Peroxisomal protein import can be analyzed using a semi-intact assay system, in which targeting efficiency is readily monitored by immunofluorescence microscopy. Furthermore, cytosolic factors required for peroxisomal protein import can be manipulated, suggesting that the semi-intact system is a useful and convenient system to uncover the molecular mechanisms of peroxisomal protein import.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages213-219
Number of pages7
DOIs
Publication statusPublished - Jan 1 2017

Publication series

NameMethods in Molecular Biology
Volume1595
ISSN (Print)1064-3745

Fingerprint

Membrane Proteins
Peroxisomes
Proteins
Plasmalogens
Metabolic Networks and Pathways
Bile Acids and Salts
Ribosomes
Fluorescence Microscopy
Organelles
Hydrogen Peroxide
Fatty Acids
Enzymes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Okumoto, K., Honsho, M., Liu, Y., & Fujiki, Y. (2017). Peroxisomal membrane and matrix protein import using a semi-intact mammalian cell system. In Methods in Molecular Biology (pp. 213-219). (Methods in Molecular Biology; Vol. 1595). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6937-1_20

Peroxisomal membrane and matrix protein import using a semi-intact mammalian cell system. / Okumoto, Kanji; Honsho, Masanori; Liu, Yuqiong; Fujiki, Yukio.

Methods in Molecular Biology. Humana Press Inc., 2017. p. 213-219 (Methods in Molecular Biology; Vol. 1595).

Research output: Chapter in Book/Report/Conference proceedingChapter

Okumoto, K, Honsho, M, Liu, Y & Fujiki, Y 2017, Peroxisomal membrane and matrix protein import using a semi-intact mammalian cell system. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1595, Humana Press Inc., pp. 213-219. https://doi.org/10.1007/978-1-4939-6937-1_20
Okumoto K, Honsho M, Liu Y, Fujiki Y. Peroxisomal membrane and matrix protein import using a semi-intact mammalian cell system. In Methods in Molecular Biology. Humana Press Inc. 2017. p. 213-219. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-6937-1_20
Okumoto, Kanji ; Honsho, Masanori ; Liu, Yuqiong ; Fujiki, Yukio. / Peroxisomal membrane and matrix protein import using a semi-intact mammalian cell system. Methods in Molecular Biology. Humana Press Inc., 2017. pp. 213-219 (Methods in Molecular Biology).
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