Persistence of hepatitis C virus RNA in established human hepatocellular carcinoma cell lines

So Tsuboi, Seishi Nagamori, Masahiro Miyazaki, Koichiro Mihara, Kenichi Fukaya, Kiichiro Teruya, Tsunenori Kosaka, Takao Tsuji, Masayoshi Namba

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The persistence of the viral RNA of hepatitis C virus (HCV) was examined in 13 hepatocellular carcinoma (HCC) and two hepatoblastoma cell lines by reverse transcription followed by the polymerase chain reaction (RT-PCR). HCV RNA was detected in three HCC lines (JHH-1, JHH-4, and JHH-6) and negative-strand viral RNA was found in JHH-4, indicating that there is a putative replicative intermediate of HCV in JHH-4 cells. To rule out the possibility of contamination, the partial nucleotide sequences of HCV-specific PCR products of these three cell lines were determined. The clone from JHH-1 belonged to genotype 1 (1 a or 1b), and the clones from JHH-4 and JHH-6 belonged to genotype 2b, but their sequences differed from each other. These cell lines may be useful for studies related to HCV.

Original languageEnglish
Pages (from-to)133-140
Number of pages8
JournalJournal of Medical Virology
Issue number2
Publication statusPublished - Feb 1 1996
Externally publishedYes


All Science Journal Classification (ASJC) codes

  • Virology
  • Infectious Diseases

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