Perspectives of germ cell development in vitro in mammals

Katsuhiko Hayashi, Mitinori Saitou

Research output: Contribution to journalReview article

19 Citations (Scopus)

Abstract

Pluripotent stem cells, such as embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) are able to differentiate into all cell lineages of the embryo proper, including germ cells. This pluripotent property has a huge impact on the fields of regenerative medicine, developmental biology and reproductive engineering. Establishing the germ cell lineage from ESCs/iPSCs is the key biological subject, since it would contribute not only to dissection of the biological processes of germ cell development but also to production of unlimited numbers of functional gametes in vitro. Toward this goal, we recently established a culture system that induces functional mouse primordial germ cells (PGCs), precursors of all germ cells, from mouse ESCs/iPSCs. The successful in vitro production of PGCs arose from the study of pluripotent cell state, the signals inducing PGCs and the technology of transplantation. However, there are many obstacles to be overcome for the robust generation of mature gametes or for application of the culture system to other species, including humans and livestock. In this review, we discuss the requirements for a culture system to generate the germ cell lineage from ESCs/iPSCs.

Original languageEnglish
Pages (from-to)617-626
Number of pages10
JournalAnimal Science Journal
Volume85
Issue number6
DOIs
Publication statusPublished - Jun 2014
Externally publishedYes

Fingerprint

Germ Cells
Mammals
germ cells
mammals
Induced Pluripotent Stem Cells
embryonic stem cells
Cell Lineage
Embryonic Stem Cells
In Vitro Techniques
Biological Phenomena
Developmental Biology
Pluripotent Stem Cells
Regenerative Medicine
Cell Transplantation
mice
Livestock
stem cells
Dissection
embryo (animal)
medicine

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)

Cite this

Perspectives of germ cell development in vitro in mammals. / Hayashi, Katsuhiko; Saitou, Mitinori.

In: Animal Science Journal, Vol. 85, No. 6, 06.2014, p. 617-626.

Research output: Contribution to journalReview article

Hayashi, Katsuhiko ; Saitou, Mitinori. / Perspectives of germ cell development in vitro in mammals. In: Animal Science Journal. 2014 ; Vol. 85, No. 6. pp. 617-626.
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