Abstract
Pluripotent stem cells, such as embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) are able to differentiate into all cell lineages of the embryo proper, including germ cells. This pluripotent property has a huge impact on the fields of regenerative medicine, developmental biology and reproductive engineering. Establishing the germ cell lineage from ESCs/iPSCs is the key biological subject, since it would contribute not only to dissection of the biological processes of germ cell development but also to production of unlimited numbers of functional gametes in vitro. Toward this goal, we recently established a culture system that induces functional mouse primordial germ cells (PGCs), precursors of all germ cells, from mouse ESCs/iPSCs. The successful in vitro production of PGCs arose from the study of pluripotent cell state, the signals inducing PGCs and the technology of transplantation. However, there are many obstacles to be overcome for the robust generation of mature gametes or for application of the culture system to other species, including humans and livestock. In this review, we discuss the requirements for a culture system to generate the germ cell lineage from ESCs/iPSCs.
| Original language | English |
|---|---|
| Pages (from-to) | 617-626 |
| Number of pages | 10 |
| Journal | Animal Science Journal |
| Volume | 85 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Jun 2014 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Agricultural and Biological Sciences(all)
Cite this
Perspectives of germ cell development in vitro in mammals. / Hayashi, Katsuhiko; Saitou, Mitinori.
In: Animal Science Journal, Vol. 85, No. 6, 06.2014, p. 617-626.Research output: Contribution to journal › Review article
}
TY - JOUR
T1 - Perspectives of germ cell development in vitro in mammals
AU - Hayashi, Katsuhiko
AU - Saitou, Mitinori
PY - 2014/6
Y1 - 2014/6
N2 - Pluripotent stem cells, such as embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) are able to differentiate into all cell lineages of the embryo proper, including germ cells. This pluripotent property has a huge impact on the fields of regenerative medicine, developmental biology and reproductive engineering. Establishing the germ cell lineage from ESCs/iPSCs is the key biological subject, since it would contribute not only to dissection of the biological processes of germ cell development but also to production of unlimited numbers of functional gametes in vitro. Toward this goal, we recently established a culture system that induces functional mouse primordial germ cells (PGCs), precursors of all germ cells, from mouse ESCs/iPSCs. The successful in vitro production of PGCs arose from the study of pluripotent cell state, the signals inducing PGCs and the technology of transplantation. However, there are many obstacles to be overcome for the robust generation of mature gametes or for application of the culture system to other species, including humans and livestock. In this review, we discuss the requirements for a culture system to generate the germ cell lineage from ESCs/iPSCs.
AB - Pluripotent stem cells, such as embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) are able to differentiate into all cell lineages of the embryo proper, including germ cells. This pluripotent property has a huge impact on the fields of regenerative medicine, developmental biology and reproductive engineering. Establishing the germ cell lineage from ESCs/iPSCs is the key biological subject, since it would contribute not only to dissection of the biological processes of germ cell development but also to production of unlimited numbers of functional gametes in vitro. Toward this goal, we recently established a culture system that induces functional mouse primordial germ cells (PGCs), precursors of all germ cells, from mouse ESCs/iPSCs. The successful in vitro production of PGCs arose from the study of pluripotent cell state, the signals inducing PGCs and the technology of transplantation. However, there are many obstacles to be overcome for the robust generation of mature gametes or for application of the culture system to other species, including humans and livestock. In this review, we discuss the requirements for a culture system to generate the germ cell lineage from ESCs/iPSCs.
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UR - http://www.scopus.com/inward/citedby.url?scp=84901834554&partnerID=8YFLogxK
U2 - 10.1111/asj.12199
DO - 10.1111/asj.12199
M3 - Review article
C2 - 24725251
AN - SCOPUS:84901834554
VL - 85
SP - 617
EP - 626
JO - Animal Science Journal
JF - Animal Science Journal
SN - 1344-3941
IS - 6
ER -