Perylenebisimide-linked nitroxide for the detection of hydroxyl radicals

Tomoharu Maki, Nobuaki Soh, Tuyoshi Fukaminato, Hizuru Nakajima, Koji Nakano, Toshihiko Imato

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

A new type of fluorophore-linked nitroxide for the detection of hydroxyl radical ({radical dot}OH) was synthesized using perylene-3,4,9,10-tetracarboxyl bisimide and 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO). This new fluorescent probe, indicated here as Spy-OH (swallow-tailed perylene derivative for detecting {radical dot}OH) has its natural fluorescence effectively reduced due to the quenching caused by the presence of the nitroxide radical in its structure. In the presence of {radical dot}OH, in media containing DMSO, a conspicuous increase in the fluorescence intensity was observed. No significant change in the fluorescence intensity was observed upon the addition of other several reactive oxygen species (ROS) except for {radical dot}OH. Spy-OH maxima excitation (λexc) and emission (λem) wavelengths lie in the visible region (520/530 nm), therefore, this fluorescent probe is expected to be useful for a simple and selective detection of {radical dot}OH, especially for biosamples.

Original languageEnglish
Pages (from-to)78-82
Number of pages5
JournalAnalytica Chimica Acta
Volume639
Issue number1-2
DOIs
Publication statusPublished - Apr 20 2009

Fingerprint

hydroxyl radical
Perylene
Hydroxyl Radical
Fluorescence
Fluorescent Dyes
Swallows
fluorescence
Fluorophores
Deglutition
Dimethyl Sulfoxide
Quenching
Reactive Oxygen Species
probe
Derivatives
Wavelength
perylene bisimide
detection
wavelength

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

Cite this

Perylenebisimide-linked nitroxide for the detection of hydroxyl radicals. / Maki, Tomoharu; Soh, Nobuaki; Fukaminato, Tuyoshi; Nakajima, Hizuru; Nakano, Koji; Imato, Toshihiko.

In: Analytica Chimica Acta, Vol. 639, No. 1-2, 20.04.2009, p. 78-82.

Research output: Contribution to journalArticle

Maki, Tomoharu ; Soh, Nobuaki ; Fukaminato, Tuyoshi ; Nakajima, Hizuru ; Nakano, Koji ; Imato, Toshihiko. / Perylenebisimide-linked nitroxide for the detection of hydroxyl radicals. In: Analytica Chimica Acta. 2009 ; Vol. 639, No. 1-2. pp. 78-82.
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