Perylenebisimide-linked nitroxide for the detection of hydroxyl radicals

Tomoharu Maki, Nobuaki Soh, Tuyoshi Fukaminato, Hizuru Nakajima, Koji Nakano, Toshihiko Imato

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

A new type of fluorophore-linked nitroxide for the detection of hydroxyl radical ({radical dot}OH) was synthesized using perylene-3,4,9,10-tetracarboxyl bisimide and 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO). This new fluorescent probe, indicated here as Spy-OH (swallow-tailed perylene derivative for detecting {radical dot}OH) has its natural fluorescence effectively reduced due to the quenching caused by the presence of the nitroxide radical in its structure. In the presence of {radical dot}OH, in media containing DMSO, a conspicuous increase in the fluorescence intensity was observed. No significant change in the fluorescence intensity was observed upon the addition of other several reactive oxygen species (ROS) except for {radical dot}OH. Spy-OH maxima excitation (λexc) and emission (λem) wavelengths lie in the visible region (520/530 nm), therefore, this fluorescent probe is expected to be useful for a simple and selective detection of {radical dot}OH, especially for biosamples.

Original languageEnglish
Pages (from-to)78-82
Number of pages5
JournalAnalytica Chimica Acta
Volume639
Issue number1-2
DOIs
Publication statusPublished - Apr 20 2009

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

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