PGC-1 α -mediated changes in phospholipid profiles of exercise-trained skeletal muscle

Nanami Senoo, Noriyuki Miyoshi, Naoko Goto-Inoue, Kimiko Minami, Ryoji Yoshimura, Akihito Morita, Naoki Sawada, Junichiro Matsuda, Yoshihiro Ogawa, Mitsutoshi Setou, Yasutomi Kamei, Shinji Miura

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Exercise training influences phospholipid fatty acid composition in skeletal muscle and these changes are associated with physiological phenotypes; however, the molecular mechanism of this influence on compositional changes is poorly understood. Peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α ), a nuclear receptor coactivator, promotes mitochondrial biogenesis, the fiber-type switch to oxidative fibers, and angiogenesis in skeletal muscle. Because exercise training induces these adaptations, together with increased PGC-1α, PGC-1α may contribute to the exercise-mediated change in phospholipid fatty acid composition. To determine the role of PGC-1α, we performed lipidomic analyses of skeletal muscle from genetically modified mice that overexpress PGC-1α in skeletal muscle or that carry KO alleles of PGC-1α . We found that PGC-1α affected lipid profiles in skeletal muscle and increased several phospholipid species in glycolytic muscle, namely phosphatidylcholine (PC) (18:0/22:6) and phosphatidylethanolamine (PE) (18:0/22:6). We also found that exercise training increased PC (18:0/22:6) and PE (18:0/22:6) in glycolytic muscle and that PGC-1α was required for these alterations. Because phospholipid fatty acid composition influences cell permeability and receptor stability at the cell membrane, these phospholipids may contribute to exercise training-mediated functional changes in the skeletal muscle.

Original languageEnglish
Pages (from-to)2286-2296
Number of pages11
JournalJournal of Lipid Research
Volume56
Issue number12
DOIs
Publication statusPublished - Dec 1 2015

Fingerprint

Muscle
Phospholipids
Skeletal Muscle
Exercise
Fatty Acids
Phosphatidylcholines
Nuclear Receptor Coactivator 1
Muscles
Peroxisome Proliferator-Activated Receptors
Nuclear Receptor Coactivators
Chemical analysis
Organelle Biogenesis
Fibers
Permeability
Cell membranes
Alleles
Cell Membrane
Phenotype
Lipids
Switches

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Endocrinology
  • Cell Biology

Cite this

Senoo, N., Miyoshi, N., Goto-Inoue, N., Minami, K., Yoshimura, R., Morita, A., ... Miura, S. (2015). PGC-1 α -mediated changes in phospholipid profiles of exercise-trained skeletal muscle. Journal of Lipid Research, 56(12), 2286-2296. https://doi.org/10.1194/jlr.M060533

PGC-1 α -mediated changes in phospholipid profiles of exercise-trained skeletal muscle. / Senoo, Nanami; Miyoshi, Noriyuki; Goto-Inoue, Naoko; Minami, Kimiko; Yoshimura, Ryoji; Morita, Akihito; Sawada, Naoki; Matsuda, Junichiro; Ogawa, Yoshihiro; Setou, Mitsutoshi; Kamei, Yasutomi; Miura, Shinji.

In: Journal of Lipid Research, Vol. 56, No. 12, 01.12.2015, p. 2286-2296.

Research output: Contribution to journalArticle

Senoo, N, Miyoshi, N, Goto-Inoue, N, Minami, K, Yoshimura, R, Morita, A, Sawada, N, Matsuda, J, Ogawa, Y, Setou, M, Kamei, Y & Miura, S 2015, 'PGC-1 α -mediated changes in phospholipid profiles of exercise-trained skeletal muscle', Journal of Lipid Research, vol. 56, no. 12, pp. 2286-2296. https://doi.org/10.1194/jlr.M060533
Senoo N, Miyoshi N, Goto-Inoue N, Minami K, Yoshimura R, Morita A et al. PGC-1 α -mediated changes in phospholipid profiles of exercise-trained skeletal muscle. Journal of Lipid Research. 2015 Dec 1;56(12):2286-2296. https://doi.org/10.1194/jlr.M060533
Senoo, Nanami ; Miyoshi, Noriyuki ; Goto-Inoue, Naoko ; Minami, Kimiko ; Yoshimura, Ryoji ; Morita, Akihito ; Sawada, Naoki ; Matsuda, Junichiro ; Ogawa, Yoshihiro ; Setou, Mitsutoshi ; Kamei, Yasutomi ; Miura, Shinji. / PGC-1 α -mediated changes in phospholipid profiles of exercise-trained skeletal muscle. In: Journal of Lipid Research. 2015 ; Vol. 56, No. 12. pp. 2286-2296.
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