PGC specification in vivo and in vitro

Research output: Contribution to journalArticle

Abstract

Primordial germ cells (PGCs), the precursors of the germ cells, arise from pluripotent epiblast cells during gastrulation in the mouse embryo. During early PGC development, there are a series of cellular events, each of which is important in the acquisition of totipotency. Pluripotent stem cells, such as embryonic stem (ES) cells, are a potential source of PGCs in vitro. Accumulating evidence suggests that ES cells can differentiate into PGCs via either embryoid body formation or monolayer cell culture. However, it remains unclear whether these in vitro-derived PGCs properly accomplish all developmental processes during PGC specification in vivo. This article reviews current studies of PGC specification both in vivo and in vitro, shelding light on the significance of PGC specification, and provides a guideline for verification in the process of PGC production in vitro.

Original languageEnglish
Pages (from-to)171-177
Number of pages7
JournalJournal of Mammalian Ova Research
Volume26
Issue number4
DOIs
Publication statusPublished - Oct 1 2009

Fingerprint

Germ Cells
Embryonic Stem Cells
In Vitro Techniques
Embryoid Bodies
Germ Layers
Pluripotent Stem Cells
Gastrulation
Embryonic Structures
Cell Culture Techniques
Guidelines
Light

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Cell Biology

Cite this

PGC specification in vivo and in vitro. / Hayashi, Katsuhiko.

In: Journal of Mammalian Ova Research, Vol. 26, No. 4, 01.10.2009, p. 171-177.

Research output: Contribution to journalArticle

@article{90867cdd90ff484587771a0415b7c994,
title = "PGC specification in vivo and in vitro",
abstract = "Primordial germ cells (PGCs), the precursors of the germ cells, arise from pluripotent epiblast cells during gastrulation in the mouse embryo. During early PGC development, there are a series of cellular events, each of which is important in the acquisition of totipotency. Pluripotent stem cells, such as embryonic stem (ES) cells, are a potential source of PGCs in vitro. Accumulating evidence suggests that ES cells can differentiate into PGCs via either embryoid body formation or monolayer cell culture. However, it remains unclear whether these in vitro-derived PGCs properly accomplish all developmental processes during PGC specification in vivo. This article reviews current studies of PGC specification both in vivo and in vitro, shelding light on the significance of PGC specification, and provides a guideline for verification in the process of PGC production in vitro.",
author = "Katsuhiko Hayashi",
year = "2009",
month = "10",
day = "1",
doi = "10.1274/jmor.26.171",
language = "English",
volume = "26",
pages = "171--177",
journal = "Journal of Mammalian Ova Research",
issn = "1341-7738",
number = "4",

}

TY - JOUR

T1 - PGC specification in vivo and in vitro

AU - Hayashi, Katsuhiko

PY - 2009/10/1

Y1 - 2009/10/1

N2 - Primordial germ cells (PGCs), the precursors of the germ cells, arise from pluripotent epiblast cells during gastrulation in the mouse embryo. During early PGC development, there are a series of cellular events, each of which is important in the acquisition of totipotency. Pluripotent stem cells, such as embryonic stem (ES) cells, are a potential source of PGCs in vitro. Accumulating evidence suggests that ES cells can differentiate into PGCs via either embryoid body formation or monolayer cell culture. However, it remains unclear whether these in vitro-derived PGCs properly accomplish all developmental processes during PGC specification in vivo. This article reviews current studies of PGC specification both in vivo and in vitro, shelding light on the significance of PGC specification, and provides a guideline for verification in the process of PGC production in vitro.

AB - Primordial germ cells (PGCs), the precursors of the germ cells, arise from pluripotent epiblast cells during gastrulation in the mouse embryo. During early PGC development, there are a series of cellular events, each of which is important in the acquisition of totipotency. Pluripotent stem cells, such as embryonic stem (ES) cells, are a potential source of PGCs in vitro. Accumulating evidence suggests that ES cells can differentiate into PGCs via either embryoid body formation or monolayer cell culture. However, it remains unclear whether these in vitro-derived PGCs properly accomplish all developmental processes during PGC specification in vivo. This article reviews current studies of PGC specification both in vivo and in vitro, shelding light on the significance of PGC specification, and provides a guideline for verification in the process of PGC production in vitro.

UR - http://www.scopus.com/inward/record.url?scp=77749254693&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77749254693&partnerID=8YFLogxK

U2 - 10.1274/jmor.26.171

DO - 10.1274/jmor.26.171

M3 - Article

AN - SCOPUS:77749254693

VL - 26

SP - 171

EP - 177

JO - Journal of Mammalian Ova Research

JF - Journal of Mammalian Ova Research

SN - 1341-7738

IS - 4

ER -