PGP9.5 methylation in diffuse-type gastric cancer

Keishi Yamashita, Hannah Lui Park, Myoung Sook Kim, Motonobu Osada, Yutaka Tokumaru, Hiroshi Inoue, Masaki Mori, David Sidransky

Research output: Contribution to journalArticle

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Abstract

Diffuse-type gastric cancer (DGC) is the most deadly form of gastric cancer and is frequently accompanied by peritoneal dissemination and metastasis. The specific molecular events involved in DGC pathogenesis remain elusive. Accumulating evidence of epigenetic inactivation in tumor suppressor genes led us to conduct a comprehensive screen to identify novel methylated genes in human cancers using pharmacologic unmasking and subsequent microarray analysis. We compared differential RNA expression profiles of DGC and intestinal-type gastric cancer (IGC) cell lines treated with 5-aza-2′-deoxycytidine using microarrays containing 22,284 genes. We identified 16 methylated genes, including many novel genes, in DGC cell lines and studied PGP9.5 with particular interest. In primary gastric cancers, PGP9.5 was found to be more frequently methylated in DGCs (78%) than in IGCs (36%; DGC versus IGC, P < 0.05). Furthermore, real-time methylation-specific PCR analysis of PGP9.5 showed relatively higher methylation levels in DGC than in IGC. Our data thus implicate a molecular event common in the DGC phenotype compared with IGC.

Original languageEnglish
Pages (from-to)3921-3927
Number of pages7
JournalCancer Research
Volume66
Issue number7
DOIs
Publication statusPublished - Apr 1 2006

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Methylation
Stomach Neoplasms
Intestinal Neoplasms
decitabine
Genes
Cell Line
Microarray Analysis
Tumor Suppressor Genes
Epigenomics
RNA
Neoplasm Metastasis
Phenotype

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Yamashita, K., Park, H. L., Kim, M. S., Osada, M., Tokumaru, Y., Inoue, H., ... Sidransky, D. (2006). PGP9.5 methylation in diffuse-type gastric cancer. Cancer Research, 66(7), 3921-3927. https://doi.org/10.1158/0008-5472.CAN-05-1511

PGP9.5 methylation in diffuse-type gastric cancer. / Yamashita, Keishi; Park, Hannah Lui; Kim, Myoung Sook; Osada, Motonobu; Tokumaru, Yutaka; Inoue, Hiroshi; Mori, Masaki; Sidransky, David.

In: Cancer Research, Vol. 66, No. 7, 01.04.2006, p. 3921-3927.

Research output: Contribution to journalArticle

Yamashita, K, Park, HL, Kim, MS, Osada, M, Tokumaru, Y, Inoue, H, Mori, M & Sidransky, D 2006, 'PGP9.5 methylation in diffuse-type gastric cancer', Cancer Research, vol. 66, no. 7, pp. 3921-3927. https://doi.org/10.1158/0008-5472.CAN-05-1511
Yamashita K, Park HL, Kim MS, Osada M, Tokumaru Y, Inoue H et al. PGP9.5 methylation in diffuse-type gastric cancer. Cancer Research. 2006 Apr 1;66(7):3921-3927. https://doi.org/10.1158/0008-5472.CAN-05-1511
Yamashita, Keishi ; Park, Hannah Lui ; Kim, Myoung Sook ; Osada, Motonobu ; Tokumaru, Yutaka ; Inoue, Hiroshi ; Mori, Masaki ; Sidransky, David. / PGP9.5 methylation in diffuse-type gastric cancer. In: Cancer Research. 2006 ; Vol. 66, No. 7. pp. 3921-3927.
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