pH-Dependent Ca 2+ interaction with phospholipids related to phosphatidylethanolamine N-methylation

Maeda Masatomo, Nishijima Masahiro, Takenaka Yumiko, Osamu Kuge, Akamatsu Yuzuru

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The interactions of PE and its N-methylated derivatives (PME, PDE and PC) with Ca 2+ were examined. PE and the intermediate phospholipids of PE N-methylation (PME and PDE) interacted with Ca 2+ in a pH-dependent and reversible manner. When these phospholipids were present in the heptane phase, Ca 2+ in the aqueous phase was translocated into the heptane phase at alkaline pH but not at acidic pH. PDE was also effective for the translocation even at around neutral pH, while PC hardly translocated Ca 2+ at pH 6.0-9.2. The amounts of Ca 2+ interacting with these phospholipids were in the following order; PDE > PME > PE > PC. PI, phosphatidic acid and PS interacted with Ca 2+ in the whole pH range examined. The Ca 2+ interactions with PI and phosphatidic acid were independent of pH, while PS interacted with more Ca 2+ at alkaline pH. These phospholipids interacted with Ca 2+ most strongly among the cations studied. Liposomes containing PDE also bound the highest amounts of Ca 2+ among PE and its N-methylated derivatives. Furthermore, mammalian cultured cell membranes, which contain increased amounts of PDE by in vivo modification with N,N'-dimethylethanolamine, bound more Ca 2+ than those prepared from choline-treated control cells.

Original languageEnglish
Pages (from-to)298-306
Number of pages9
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume794
Issue number2
DOIs
Publication statusPublished - Jul 6 1984
Externally publishedYes

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Methylation
Phospholipids
Heptanes
Phosphatidic Acids
Deanol
Derivatives
Cell membranes
Choline
Liposomes
Cations
phosphatidylethanolamine
Cultured Cells
Cell Membrane

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Endocrinology

Cite this

pH-Dependent Ca 2+ interaction with phospholipids related to phosphatidylethanolamine N-methylation . / Masatomo, Maeda; Masahiro, Nishijima; Yumiko, Takenaka; Kuge, Osamu; Yuzuru, Akamatsu.

In: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism, Vol. 794, No. 2, 06.07.1984, p. 298-306.

Research output: Contribution to journalArticle

Masatomo, Maeda ; Masahiro, Nishijima ; Yumiko, Takenaka ; Kuge, Osamu ; Yuzuru, Akamatsu. / pH-Dependent Ca 2+ interaction with phospholipids related to phosphatidylethanolamine N-methylation In: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism. 1984 ; Vol. 794, No. 2. pp. 298-306.
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