PH-responsive release of proteins from biocompatible and biodegradable reverse polymer micelles

Yuichi Koyamatsu, Taisuke Hirano, Yoshinori Kakizawa, Fumiyoshi Okano, Tohru Takarada, Mizuo Maeda

Research output: Contribution to journalArticle

48 Citations (Scopus)


A reverse polymer micelle with a diameter of 100 nm was prepared for a protein carrier releasing payloads in a pH-dependent manner. The reverse polymer micelle was made from an amphiphilic diblock copolymer of biodegradable poly(d,l-lactic-co-glycolic acid) (PLGA) and biocompatible poly(ethylene glycol) (PEG). PLGA having a terminal carboxyl group was additionally embedded in the micelle's PLGA layer via hydrophobic interaction. The micelles encapsulating bovine serum albumin and streptavidin released the proteins under neutral and basic conditions, whereas the proteins remained in the interior at acidic pH. Using erythropoietin as a protein drug, it was also exemplified that the released protein retained its cell proliferation activity even after rigorous formulation processes, including water-in-oil emulsion. The present reverse polymer micelle could potentially find application as an oral protein drug delivery carrier.

Original languageEnglish
Pages (from-to)89-95
Number of pages7
JournalJournal of Controlled Release
Issue number1
Publication statusPublished - Jan 1 2014
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

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