Phagocytosis is mediated by two-dimensional assemblies of the F-BAR protein GAS7

Kyoko Hanawa-Suetsugu, Yuzuru Itoh, Maisarah Ab Fatah, Tamako Nishimura, Kazuhiro Takemura, Kohei Takeshita, Satoru Kubota, Naoyuki Miyazaki, Wan Nurul Izzati Wan Mohamad Noor, Takehiko Inaba, Nhung Thi Hong Nguyen, Sayaka Hamada-Nakahara, Kayoko Oono-Yakura, Masashi Tachikawa, Kenji Iwasaki, Daisuke Kohda, Masaki Yamamoto, Akio Kitao, Atsushi Shimada, Shiro Suetsugu

Research output: Contribution to journalArticle

Abstract

Phagocytosis is a cellular process for internalization of micron-sized large particles including pathogens. The Bin-Amphiphysin-Rvs167 (BAR) domain proteins, including the FCH-BAR (F-BAR) domain proteins, impose specific morphologies on lipid membranes. Most BAR domain proteins are thought to form membrane invaginations or protrusions by assembling into helical submicron-diameter filaments, such as on clathrin-coated pits, caveolae, and filopodia. However, the mechanism by which BAR domain proteins assemble into micron-scale phagocytic cups was unclear. Here, we show that the two-dimensional sheet-like assembly of Growth Arrest-Specific 7 (GAS7) plays a critical role in phagocytic cup formation in macrophages. GAS7 has the F-BAR domain that possesses unique hydrophilic loops for two-dimensional sheet formation on flat membranes. Super-resolution microscopy reveals the similar assemblies of GAS7 on phagocytic cups and liposomes. The mutations of the loops abolishes both the membrane localization of GAS7 and phagocytosis. Thus, the sheet-like assembly of GAS7 plays a significant role in phagocytosis.

Original languageEnglish
Article number4763
JournalNature communications
Volume10
Issue number1
DOIs
Publication statusPublished - Dec 1 2019

Fingerprint

Phagocytosis
assemblies
Bins
proteins
membranes
Growth
Proteins
Membranes
assembly
Caveolae
Clathrin
Pseudopodia
macrophages
pathogens
Macrophages
Pathogens
Membrane Lipids
mutations
assembling
Liposomes

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Hanawa-Suetsugu, K., Itoh, Y., Ab Fatah, M., Nishimura, T., Takemura, K., Takeshita, K., ... Suetsugu, S. (2019). Phagocytosis is mediated by two-dimensional assemblies of the F-BAR protein GAS7. Nature communications, 10(1), [4763]. https://doi.org/10.1038/s41467-019-12738-w

Phagocytosis is mediated by two-dimensional assemblies of the F-BAR protein GAS7. / Hanawa-Suetsugu, Kyoko; Itoh, Yuzuru; Ab Fatah, Maisarah; Nishimura, Tamako; Takemura, Kazuhiro; Takeshita, Kohei; Kubota, Satoru; Miyazaki, Naoyuki; Wan Mohamad Noor, Wan Nurul Izzati; Inaba, Takehiko; Nguyen, Nhung Thi Hong; Hamada-Nakahara, Sayaka; Oono-Yakura, Kayoko; Tachikawa, Masashi; Iwasaki, Kenji; Kohda, Daisuke; Yamamoto, Masaki; Kitao, Akio; Shimada, Atsushi; Suetsugu, Shiro.

In: Nature communications, Vol. 10, No. 1, 4763, 01.12.2019.

Research output: Contribution to journalArticle

Hanawa-Suetsugu, K, Itoh, Y, Ab Fatah, M, Nishimura, T, Takemura, K, Takeshita, K, Kubota, S, Miyazaki, N, Wan Mohamad Noor, WNI, Inaba, T, Nguyen, NTH, Hamada-Nakahara, S, Oono-Yakura, K, Tachikawa, M, Iwasaki, K, Kohda, D, Yamamoto, M, Kitao, A, Shimada, A & Suetsugu, S 2019, 'Phagocytosis is mediated by two-dimensional assemblies of the F-BAR protein GAS7', Nature communications, vol. 10, no. 1, 4763. https://doi.org/10.1038/s41467-019-12738-w
Hanawa-Suetsugu K, Itoh Y, Ab Fatah M, Nishimura T, Takemura K, Takeshita K et al. Phagocytosis is mediated by two-dimensional assemblies of the F-BAR protein GAS7. Nature communications. 2019 Dec 1;10(1). 4763. https://doi.org/10.1038/s41467-019-12738-w
Hanawa-Suetsugu, Kyoko ; Itoh, Yuzuru ; Ab Fatah, Maisarah ; Nishimura, Tamako ; Takemura, Kazuhiro ; Takeshita, Kohei ; Kubota, Satoru ; Miyazaki, Naoyuki ; Wan Mohamad Noor, Wan Nurul Izzati ; Inaba, Takehiko ; Nguyen, Nhung Thi Hong ; Hamada-Nakahara, Sayaka ; Oono-Yakura, Kayoko ; Tachikawa, Masashi ; Iwasaki, Kenji ; Kohda, Daisuke ; Yamamoto, Masaki ; Kitao, Akio ; Shimada, Atsushi ; Suetsugu, Shiro. / Phagocytosis is mediated by two-dimensional assemblies of the F-BAR protein GAS7. In: Nature communications. 2019 ; Vol. 10, No. 1.
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