TY - JOUR
T1 - Pharmacokinetic significance of renal OAT3 (SLC22A8) for anionic drug elimination in patients with mesangial proliferative glomerulonephritis
AU - Sakurai, Yuji
AU - Motohashi, Hideyuki
AU - Ogasawara, Ken
AU - Terada, Tomohiro
AU - Masuda, Satohiro
AU - Katsura, Toshiya
AU - Mori, Noriko
AU - Matsuura, Motokazu
AU - Doi, Toshio
AU - Fukatsu, Atsushi
AU - Inui, Ken Ichi
N1 - Funding Information:
This work was supported by a grant-in-aid for Comprehensive Research on Aging and Health from the Ministry of Health and Welfare of Japan (H15-Choju-006), by a grant-in-aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan, by a grant-in-aid from the Japan Research Foundation for Clinical Pharmacology, and by the 21st Century COE program BKnowledge Information Infrastructure for Genome Science.^
PY - 2005/12
Y1 - 2005/12
N2 - Purpose. Our previous studies showed that the mRNA level of human organic anion transporter (hOAT) 3 in the kidney was correlated with the rate of elimination of an anionic antibiotic cefazolin. However, the correlation coefficient was not so high. In the present study, therefore, we enrolled more patients to examine whether additional factors were responsible for the correlation. Methods. hOAT mRNA levels in renal biopsy specimens were quantified using the real-time polymerase chain reaction method. The elimination rates for the free fraction of cefazolin were determined in patients with various renal diseases. Results. In the present study, the coefficient of correlation between the hOAT3 mRNA level and the elimination rates for the free fraction of cefazolin was not so high in the patients overall as in our previous study (r = 0.536). However, following the classification of renal diseases, a better correlation was obtained in patients with mesangial proliferative glomerulonephritis (r = 0.723). In contrast, multiple regression analyses including gender, age, and liver function did not result in any improvements in the correlation coefficients. Conclusions. These results suggest that the hOAT3 mRNA level is a significant marker of pharmacokinetics with which to predict the rate of elimination of cefazolin in patients with mesangial proliferative glomerulonephritis.
AB - Purpose. Our previous studies showed that the mRNA level of human organic anion transporter (hOAT) 3 in the kidney was correlated with the rate of elimination of an anionic antibiotic cefazolin. However, the correlation coefficient was not so high. In the present study, therefore, we enrolled more patients to examine whether additional factors were responsible for the correlation. Methods. hOAT mRNA levels in renal biopsy specimens were quantified using the real-time polymerase chain reaction method. The elimination rates for the free fraction of cefazolin were determined in patients with various renal diseases. Results. In the present study, the coefficient of correlation between the hOAT3 mRNA level and the elimination rates for the free fraction of cefazolin was not so high in the patients overall as in our previous study (r = 0.536). However, following the classification of renal diseases, a better correlation was obtained in patients with mesangial proliferative glomerulonephritis (r = 0.723). In contrast, multiple regression analyses including gender, age, and liver function did not result in any improvements in the correlation coefficients. Conclusions. These results suggest that the hOAT3 mRNA level is a significant marker of pharmacokinetics with which to predict the rate of elimination of cefazolin in patients with mesangial proliferative glomerulonephritis.
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U2 - 10.1007/s11095-005-8383-5
DO - 10.1007/s11095-005-8383-5
M3 - Article
C2 - 16247710
AN - SCOPUS:29244454212
VL - 22
SP - 2016
EP - 2022
JO - Pharmaceutical Research
JF - Pharmaceutical Research
SN - 0724-8741
IS - 12
ER -