Phase behaviors of agarose gel

Takaharu Morita, Takayuki Narita, Sada Atsu Mukai, Miho Yanagisawa, Masayuki Tokita

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We present evidence for the existence of phase separation in the gel state of agarose having the mixture of water and methanol as the gel solvent. Firstly, the sol-gel transition line and the cloud point line are determined independently as a function of the concentration of agarose as well as the concentration of methanol in the mixed solvent by the quasi-equilibrium cooling of the solutions. Then the spinodal line is determined by quenching the solutions below the sol-gel transition line. We find that the spinodal line appears below the cloud point line and both lines are entirely buried below the sol-gel transition line in the aqueous agarose system. The concentration fluctuations are, therefore, frozen into the polymer network of agarose gel that promotes the opacity of the resultant gel. The structure of agarose gel is observed by the confocal laser scanning microscope (CLSM) imaging technique that reveals that the density fluctuations are grown up to micrometer scale in space. The phase separation boundary is found to shift to the higher temperature region than the sol-gel transition line when the concentration of methanol in the mixed solvent is increased. The results indicate that the position of the phase separation boundary in relative to the sol-gel transition line varies with the quality of solvent. These results are in agreement with the theory of the sol-gel transition in which both the divergence of the connectivity and the thermodynamic instability are taken into account.

Original languageEnglish
Article number042128
JournalAIP Advances
Volume3
Issue number4
DOIs
Publication statusPublished - May 15 2013

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gels
methyl alcohol
opacity
imaging techniques
micrometers
divergence
quenching
microscopes
cooling
thermodynamics
scanning
shift
polymers
water
lasers

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy(all)

Cite this

Morita, T., Narita, T., Mukai, S. A., Yanagisawa, M., & Tokita, M. (2013). Phase behaviors of agarose gel. AIP Advances, 3(4), [042128]. https://doi.org/10.1063/1.4802968

Phase behaviors of agarose gel. / Morita, Takaharu; Narita, Takayuki; Mukai, Sada Atsu; Yanagisawa, Miho; Tokita, Masayuki.

In: AIP Advances, Vol. 3, No. 4, 042128, 15.05.2013.

Research output: Contribution to journalArticle

Morita, T, Narita, T, Mukai, SA, Yanagisawa, M & Tokita, M 2013, 'Phase behaviors of agarose gel', AIP Advances, vol. 3, no. 4, 042128. https://doi.org/10.1063/1.4802968
Morita T, Narita T, Mukai SA, Yanagisawa M, Tokita M. Phase behaviors of agarose gel. AIP Advances. 2013 May 15;3(4). 042128. https://doi.org/10.1063/1.4802968
Morita, Takaharu ; Narita, Takayuki ; Mukai, Sada Atsu ; Yanagisawa, Miho ; Tokita, Masayuki. / Phase behaviors of agarose gel. In: AIP Advances. 2013 ; Vol. 3, No. 4.
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