Phase separation multi-phase flow using an aqueous two-phase system of a polyethylene glycol/dextran mixed solution

Naoya Imanishi, Tetsuo Yamasaki, Kazuhiko Tsukagoshi, Masaharu Murata

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Polyethylene glycol/dextran mixed solution as an aqueous two-phase system was fed into a fused-silica capillary tube under different conditions, resulting in phase transformation leading to phase separation multi-phase flow through/along a liquid-liquid interface. As one flow-type example, when 6.4 wt% polyethylene glycol and 9.7 wt% dextran aqueous solution containing 1.0 mM Rhodamine B was fed into the capillary tube at 3°C, tube radial distribution flow (annual flow) was observed through bright-field microscopy. Tube radial distribution flow consisted of a dextran-rich inner phase and polyethylene glycol-rich outer phase. We also examined the distribution of proteins, such as bovine serum albumin, hemoglobin, and lysozyme, in the inner and outer phases through use of double capillary tubes with different inner diameters. The protein distribution was greater in the inner (dextran-rich) phase than the outer (polyethylene glycol-rich) phase. The distribution ratios of the three proteins (ratio of the inner/outer protein concentration) were 2.3, 4.2, and 1.8, respectively. The proteins concentrated in the dextran-rich phase through tube radial distribution flow of a polyethylene glycol/dextran mixed solution.

Original languageEnglish
Pages (from-to)953-958
Number of pages6
Journalanalytical sciences
Volume34
Issue number8
DOIs
Publication statusPublished - 2018

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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