Abstract
The phenol-sulfate conjugation by the liver subcellular fractions from goldfish Carassius auratus, carp Cyprinus carpio, rainbow trout Salmo gairdneri, and Tilapia nilotica has been studied using [35S] K2SO4. The cell fractionation of goldfish liver was examined by the determination of respective markers for nuclear, mitochondrial, lysosomal, microsomal, and soluble fractions. Among those subcellular fractions, only the soluble fraction displayed the conjugating activity. The maximum activity of the enzyme in the soluble fraction was obtained in the presence of 20 µmol of both ATP and Mg2+ in 1.5 ml of medium, although the activity decreased with an excess of ATP and Mg2+. This conjugation was inhibited by more than 0.1 µmol of Ca2+ in 1.5 ml of medium. The enzyme exhibited the conjugating activity in the pH range 7.5–9.5; the optimum value was at 8. In the temperature range 5–40°C, the maximum conjugation in goldfish, Tilapia, and carp occurred at 35°C, in rainbow trout it occurred at 30°C. The phenol-sulfate conjugating activities in goldfish, carp, rainbow trout, Tilapia and albino rat were approximately 340, 82, 66, 200 and 720 nmol/g-liver per h, respectively.
| Original language | English |
|---|---|
| Pages (from-to) | 433-437 |
| Number of pages | 5 |
| Journal | NIPPON SUISAN GAKKAISHI |
| Volume | 48 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Jan 1 1982 |
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All Science Journal Classification (ASJC) codes
- Aquatic Science
Cite this
Phenol-Sulfate Conjugating Activity in Liver-Soluble Fraction of Fish. / Kimura, Shigeo; Oshima, Yuji; Kobayashi, Kunio.
In: NIPPON SUISAN GAKKAISHI, Vol. 48, No. 3, 01.01.1982, p. 433-437.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Phenol-Sulfate Conjugating Activity in Liver-Soluble Fraction of Fish
AU - Kimura, Shigeo
AU - Oshima, Yuji
AU - Kobayashi, Kunio
PY - 1982/1/1
Y1 - 1982/1/1
N2 - The phenol-sulfate conjugation by the liver subcellular fractions from goldfish Carassius auratus, carp Cyprinus carpio, rainbow trout Salmo gairdneri, and Tilapia nilotica has been studied using [35S] K2SO4. The cell fractionation of goldfish liver was examined by the determination of respective markers for nuclear, mitochondrial, lysosomal, microsomal, and soluble fractions. Among those subcellular fractions, only the soluble fraction displayed the conjugating activity. The maximum activity of the enzyme in the soluble fraction was obtained in the presence of 20 µmol of both ATP and Mg2+ in 1.5 ml of medium, although the activity decreased with an excess of ATP and Mg2+. This conjugation was inhibited by more than 0.1 µmol of Ca2+ in 1.5 ml of medium. The enzyme exhibited the conjugating activity in the pH range 7.5–9.5; the optimum value was at 8. In the temperature range 5–40°C, the maximum conjugation in goldfish, Tilapia, and carp occurred at 35°C, in rainbow trout it occurred at 30°C. The phenol-sulfate conjugating activities in goldfish, carp, rainbow trout, Tilapia and albino rat were approximately 340, 82, 66, 200 and 720 nmol/g-liver per h, respectively.
AB - The phenol-sulfate conjugation by the liver subcellular fractions from goldfish Carassius auratus, carp Cyprinus carpio, rainbow trout Salmo gairdneri, and Tilapia nilotica has been studied using [35S] K2SO4. The cell fractionation of goldfish liver was examined by the determination of respective markers for nuclear, mitochondrial, lysosomal, microsomal, and soluble fractions. Among those subcellular fractions, only the soluble fraction displayed the conjugating activity. The maximum activity of the enzyme in the soluble fraction was obtained in the presence of 20 µmol of both ATP and Mg2+ in 1.5 ml of medium, although the activity decreased with an excess of ATP and Mg2+. This conjugation was inhibited by more than 0.1 µmol of Ca2+ in 1.5 ml of medium. The enzyme exhibited the conjugating activity in the pH range 7.5–9.5; the optimum value was at 8. In the temperature range 5–40°C, the maximum conjugation in goldfish, Tilapia, and carp occurred at 35°C, in rainbow trout it occurred at 30°C. The phenol-sulfate conjugating activities in goldfish, carp, rainbow trout, Tilapia and albino rat were approximately 340, 82, 66, 200 and 720 nmol/g-liver per h, respectively.
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UR - http://www.scopus.com/inward/citedby.url?scp=85008009613&partnerID=8YFLogxK
U2 - 10.2331/suisan.48.433
DO - 10.2331/suisan.48.433
M3 - Article
AN - SCOPUS:85008009613
VL - 48
SP - 433
EP - 437
JO - Nippon Suisan Gakkaishi
JF - Nippon Suisan Gakkaishi
SN - 0021-5392
IS - 3
ER -