Phenotype-related alteration in expression of natriuretic peptide receptors in aortic smooth muscle cells

S. I. Suga, K. Nakao, I. Kishimoto, K. Hosoda, M. Mukoyama, H. Arai, G. Shirakami, Yoshihiro Ogawa, Y. Komatsu, O. Nakagawa, N. Hama, H. Imura

Research output: Contribution to journalArticle

111 Citations (Scopus)

Abstract

To elucidate the physiological and pathophysiological roles of the natriuretic peptide family in vascular smooth muscle cells, in which the natriuretic peptide family is implicated in growth inhibition as well as vasorelaxation, we have examined the phenotype-related expression of three kinds of natriuretic peptide receptors in rat aortic smooth muscle cells. The expression of natriuretic peptide receptors at the mRNA level was studied by Northern blot hybridization, and the expression at the protein level was determined by the cGMP production method and receptor binding assay. In intact aortic media, atrial natriuretic peptide (ANP)-A receptor mRNA and ANP-B receptor mRNA were detected, and the potency of cGMP production by ANP was at least two orders of magnitude stronger than that by C-type natriuretic peptide. Clearance receptor mRNA was undetectable, and only a small amount of the clearance receptor was detected by the binding assay in intact aortic media. By contrast, in cultured aortic smooth muscle cells at the first, fifth, and 17th passages, the ANP-B receptor mRNA level markedly increased; meanwhile, the expression of the ANP-A receptor mRNA became undetectable. C- type natriuretic peptide was one order of magnitude more potent than ANP in cGMP production in cultured aortic smooth muscle cells. The clearance receptor density and its mRNA level increased tremendously in these cultured cells. These results demonstrate that the marked phenotype-related alteration occurs in the expression of natriuretic peptide receptors in rat aortic smooth muscle cells.

Original languageEnglish
Pages (from-to)34-39
Number of pages6
JournalCirculation research
Volume71
Issue number1
DOIs
Publication statusPublished - Jan 1 1992
Externally publishedYes

Fingerprint

Natriuretic Peptides
Peptide Receptors
Smooth Muscle Myocytes
Atrial Natriuretic Factor Receptors
Phenotype
Messenger RNA
C-Type Natriuretic Peptide
Atrial Natriuretic Factor
Vascular Smooth Muscle
Vasodilation
Northern Blotting
Cultured Cells
Growth

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Suga, S. I., Nakao, K., Kishimoto, I., Hosoda, K., Mukoyama, M., Arai, H., ... Imura, H. (1992). Phenotype-related alteration in expression of natriuretic peptide receptors in aortic smooth muscle cells. Circulation research, 71(1), 34-39. https://doi.org/10.1161/01.RES.71.1.34

Phenotype-related alteration in expression of natriuretic peptide receptors in aortic smooth muscle cells. / Suga, S. I.; Nakao, K.; Kishimoto, I.; Hosoda, K.; Mukoyama, M.; Arai, H.; Shirakami, G.; Ogawa, Yoshihiro; Komatsu, Y.; Nakagawa, O.; Hama, N.; Imura, H.

In: Circulation research, Vol. 71, No. 1, 01.01.1992, p. 34-39.

Research output: Contribution to journalArticle

Suga, SI, Nakao, K, Kishimoto, I, Hosoda, K, Mukoyama, M, Arai, H, Shirakami, G, Ogawa, Y, Komatsu, Y, Nakagawa, O, Hama, N & Imura, H 1992, 'Phenotype-related alteration in expression of natriuretic peptide receptors in aortic smooth muscle cells', Circulation research, vol. 71, no. 1, pp. 34-39. https://doi.org/10.1161/01.RES.71.1.34
Suga, S. I. ; Nakao, K. ; Kishimoto, I. ; Hosoda, K. ; Mukoyama, M. ; Arai, H. ; Shirakami, G. ; Ogawa, Yoshihiro ; Komatsu, Y. ; Nakagawa, O. ; Hama, N. ; Imura, H. / Phenotype-related alteration in expression of natriuretic peptide receptors in aortic smooth muscle cells. In: Circulation research. 1992 ; Vol. 71, No. 1. pp. 34-39.
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