Phenotypic and genetic analyses of T-cell-mediated immunoregulation in patients with Type 1 diabetes

Y. Tsutsumi, X. Jie, K. Ihara, A. Nomura, S. Kanemitsu, Hidetoshi Takada, T. Hara

Research output: Contribution to journalArticle

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Abstract

Aims: To investigate the contribution of regulatory T cells and co-stimulatory molecules in CD4+ T cells to the development of Type 1 diabetes (T1D). Methods: Twelve patients with T1D, nine patients with systemic lupus erythematosus (SLE), and 12 age-matched healthy control subjects participated. We analysed the proportions of CD25+CD4+ T cells and natural killer T cells (NKT cells), and the expression levels of Foxp3, CTLA-4, CD28, ICOS, PD-1 and BTLA in peripheral blood mononuclear cells and purified CD4+ T cells. Results: There were no significant differences in the proportions of CD25+ CD4+ T cells or NKT cells among the three groups. PD-1 expression levels of peripheral CD4 + T cells from T1D patients were significantly lower than those from healthy control subjects (P = 0.00066). In contrast, PD-1 expression levels were similar in SLE patients and healthy control subjects. The expression levels of Foxp3, CTLA-4, CD28, ICOS and BTLA were similar in the three groups. Conclusions: Decreased expression of the PD-1 gene in CD4+ T cells may contribute to the development and/or maintenance of autoimmune T1D. As the population studied was small and heterogeneous, further studies are required to confirm the findings.

Original languageEnglish
Pages (from-to)1145-1150
Number of pages6
JournalDiabetic Medicine
Volume23
Issue number10
DOIs
Publication statusPublished - Oct 1 2006

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Type 1 Diabetes Mellitus
T-Lymphocytes
Healthy Volunteers
Natural Killer T-Cells
Systemic Lupus Erythematosus
CD4 Antigens
Regulatory T-Lymphocytes
Blood Cells
Maintenance
Population
Genes

All Science Journal Classification (ASJC) codes

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Tsutsumi, Y., Jie, X., Ihara, K., Nomura, A., Kanemitsu, S., Takada, H., & Hara, T. (2006). Phenotypic and genetic analyses of T-cell-mediated immunoregulation in patients with Type 1 diabetes. Diabetic Medicine, 23(10), 1145-1150. https://doi.org/10.1111/j.1464-5491.2006.01951.x

Phenotypic and genetic analyses of T-cell-mediated immunoregulation in patients with Type 1 diabetes. / Tsutsumi, Y.; Jie, X.; Ihara, K.; Nomura, A.; Kanemitsu, S.; Takada, Hidetoshi; Hara, T.

In: Diabetic Medicine, Vol. 23, No. 10, 01.10.2006, p. 1145-1150.

Research output: Contribution to journalArticle

Tsutsumi, Y, Jie, X, Ihara, K, Nomura, A, Kanemitsu, S, Takada, H & Hara, T 2006, 'Phenotypic and genetic analyses of T-cell-mediated immunoregulation in patients with Type 1 diabetes', Diabetic Medicine, vol. 23, no. 10, pp. 1145-1150. https://doi.org/10.1111/j.1464-5491.2006.01951.x
Tsutsumi, Y. ; Jie, X. ; Ihara, K. ; Nomura, A. ; Kanemitsu, S. ; Takada, Hidetoshi ; Hara, T. / Phenotypic and genetic analyses of T-cell-mediated immunoregulation in patients with Type 1 diabetes. In: Diabetic Medicine. 2006 ; Vol. 23, No. 10. pp. 1145-1150.
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