Abstract
Aims: To investigate the contribution of regulatory T cells and co-stimulatory molecules in CD4+ T cells to the development of Type 1 diabetes (T1D). Methods: Twelve patients with T1D, nine patients with systemic lupus erythematosus (SLE), and 12 age-matched healthy control subjects participated. We analysed the proportions of CD25+CD4+ T cells and natural killer T cells (NKT cells), and the expression levels of Foxp3, CTLA-4, CD28, ICOS, PD-1 and BTLA in peripheral blood mononuclear cells and purified CD4+ T cells. Results: There were no significant differences in the proportions of CD25+ CD4+ T cells or NKT cells among the three groups. PD-1 expression levels of peripheral CD4 + T cells from T1D patients were significantly lower than those from healthy control subjects (P = 0.00066). In contrast, PD-1 expression levels were similar in SLE patients and healthy control subjects. The expression levels of Foxp3, CTLA-4, CD28, ICOS and BTLA were similar in the three groups. Conclusions: Decreased expression of the PD-1 gene in CD4+ T cells may contribute to the development and/or maintenance of autoimmune T1D. As the population studied was small and heterogeneous, further studies are required to confirm the findings.
| Original language | English |
|---|---|
| Pages (from-to) | 1145-1150 |
| Number of pages | 6 |
| Journal | Diabetic Medicine |
| Volume | 23 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - Oct 1 2006 |
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All Science Journal Classification (ASJC) codes
- Internal Medicine
- Endocrinology, Diabetes and Metabolism
- Endocrinology
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Phenotypic and genetic analyses of T-cell-mediated immunoregulation in patients with Type 1 diabetes. / Tsutsumi, Y.; Jie, X.; Ihara, K.; Nomura, A.; Kanemitsu, S.; Takada, H.; Hara, T.
In: Diabetic Medicine, Vol. 23, No. 10, 01.10.2006, p. 1145-1150.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Phenotypic and genetic analyses of T-cell-mediated immunoregulation in patients with Type 1 diabetes
AU - Tsutsumi, Y.
AU - Jie, X.
AU - Ihara, K.
AU - Nomura, A.
AU - Kanemitsu, S.
AU - Takada, H.
AU - Hara, T.
PY - 2006/10/1
Y1 - 2006/10/1
N2 - Aims: To investigate the contribution of regulatory T cells and co-stimulatory molecules in CD4+ T cells to the development of Type 1 diabetes (T1D). Methods: Twelve patients with T1D, nine patients with systemic lupus erythematosus (SLE), and 12 age-matched healthy control subjects participated. We analysed the proportions of CD25+CD4+ T cells and natural killer T cells (NKT cells), and the expression levels of Foxp3, CTLA-4, CD28, ICOS, PD-1 and BTLA in peripheral blood mononuclear cells and purified CD4+ T cells. Results: There were no significant differences in the proportions of CD25+ CD4+ T cells or NKT cells among the three groups. PD-1 expression levels of peripheral CD4 + T cells from T1D patients were significantly lower than those from healthy control subjects (P = 0.00066). In contrast, PD-1 expression levels were similar in SLE patients and healthy control subjects. The expression levels of Foxp3, CTLA-4, CD28, ICOS and BTLA were similar in the three groups. Conclusions: Decreased expression of the PD-1 gene in CD4+ T cells may contribute to the development and/or maintenance of autoimmune T1D. As the population studied was small and heterogeneous, further studies are required to confirm the findings.
AB - Aims: To investigate the contribution of regulatory T cells and co-stimulatory molecules in CD4+ T cells to the development of Type 1 diabetes (T1D). Methods: Twelve patients with T1D, nine patients with systemic lupus erythematosus (SLE), and 12 age-matched healthy control subjects participated. We analysed the proportions of CD25+CD4+ T cells and natural killer T cells (NKT cells), and the expression levels of Foxp3, CTLA-4, CD28, ICOS, PD-1 and BTLA in peripheral blood mononuclear cells and purified CD4+ T cells. Results: There were no significant differences in the proportions of CD25+ CD4+ T cells or NKT cells among the three groups. PD-1 expression levels of peripheral CD4 + T cells from T1D patients were significantly lower than those from healthy control subjects (P = 0.00066). In contrast, PD-1 expression levels were similar in SLE patients and healthy control subjects. The expression levels of Foxp3, CTLA-4, CD28, ICOS and BTLA were similar in the three groups. Conclusions: Decreased expression of the PD-1 gene in CD4+ T cells may contribute to the development and/or maintenance of autoimmune T1D. As the population studied was small and heterogeneous, further studies are required to confirm the findings.
UR - http://www.scopus.com/inward/record.url?scp=33748745463&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33748745463&partnerID=8YFLogxK
U2 - 10.1111/j.1464-5491.2006.01951.x
DO - 10.1111/j.1464-5491.2006.01951.x
M3 - Article
C2 - 16978382
AN - SCOPUS:33748745463
VL - 23
SP - 1145
EP - 1150
JO - Diabetic Medicine
JF - Diabetic Medicine
SN - 0742-3071
IS - 10
ER -