Phenotypic characterization and virulence-related properties of Escherichia albertii strains isolated from children with diarrhea in Brazil

Mauricio P. Lima, Denise Yamamoto, Ana Carolina de Mello Santos, Tadasuke Ooka, Rodrigo T. Hernandes, Mônica A.M. Vieira, Fernanda Fernandes Santos, Rosa Maria Silva, Tetsuya Hayashi, Tânia A.T. Gomes

Research output: Contribution to journalArticle

Abstract

Escherichia albertii are emerging enteropathogens, whose identification is difficult, as they share biochemical characteristics and some virulence-related genes with diarrheagenic Escherichia coli (DEC). Studies on phylogeny, phenotypic characteristics and potential virulence factors of human E. albertii strains are scarce. In this study, we identified by multiplex PCR five E. albertii among 106 strains isolated from diarrheic children in São Paulo, Brazil, which were previously classified as atypical enteropathogenic E. coli. All strains were investigated regarding their phylogeny, biochemical properties, virulence-related properties, antimicrobial resistance and presence of putative virulence-related genes. All strains belonged to different E. albertii lineages and adhered to and produced attaching and effacing lesions on HeLa cells. Three strains invaded Caco-2 cells, but did not persist intracellularly, and three formed biofilms on polystyrene surfaces. All strains were resistant to few antibiotics and only one carried a self-transmissible resistance plasmid. Finally, among 38 DEC and 18 extraintestinal pathogenic E. coli (ExPEC) virulence-related genes searched, six and three were detected, respectively, with paa and cdtB being found in all strains. Despite the limited number of strains, this study provided additional knowledge on human E. albertii virulence potential, showing that they share important virulence factors with DEC and ExPEC.

Original languageEnglish
JournalPathogens and disease
Volume77
Issue number2
DOIs
Publication statusPublished - Mar 1 2019

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Escherichia
Brazil
Virulence
Diarrhea
Virulence Factors
Phylogeny
Escherichia coli
Genes
Enteropathogenic Escherichia coli
Caco-2 Cells
Multiplex Polymerase Chain Reaction
Polystyrenes
Biofilms
HeLa Cells
Plasmids
Anti-Bacterial Agents

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Lima, M. P., Yamamoto, D., de Mello Santos, A. C., Ooka, T., Hernandes, R. T., Vieira, M. A. M., ... Gomes, T. A. T. (2019). Phenotypic characterization and virulence-related properties of Escherichia albertii strains isolated from children with diarrhea in Brazil. Pathogens and disease, 77(2). https://doi.org/10.1093/femspd/ftz014

Phenotypic characterization and virulence-related properties of Escherichia albertii strains isolated from children with diarrhea in Brazil. / Lima, Mauricio P.; Yamamoto, Denise; de Mello Santos, Ana Carolina; Ooka, Tadasuke; Hernandes, Rodrigo T.; Vieira, Mônica A.M.; Santos, Fernanda Fernandes; Silva, Rosa Maria; Hayashi, Tetsuya; Gomes, Tânia A.T.

In: Pathogens and disease, Vol. 77, No. 2, 01.03.2019.

Research output: Contribution to journalArticle

Lima, MP, Yamamoto, D, de Mello Santos, AC, Ooka, T, Hernandes, RT, Vieira, MAM, Santos, FF, Silva, RM, Hayashi, T & Gomes, TAT 2019, 'Phenotypic characterization and virulence-related properties of Escherichia albertii strains isolated from children with diarrhea in Brazil', Pathogens and disease, vol. 77, no. 2. https://doi.org/10.1093/femspd/ftz014
Lima, Mauricio P. ; Yamamoto, Denise ; de Mello Santos, Ana Carolina ; Ooka, Tadasuke ; Hernandes, Rodrigo T. ; Vieira, Mônica A.M. ; Santos, Fernanda Fernandes ; Silva, Rosa Maria ; Hayashi, Tetsuya ; Gomes, Tânia A.T. / Phenotypic characterization and virulence-related properties of Escherichia albertii strains isolated from children with diarrhea in Brazil. In: Pathogens and disease. 2019 ; Vol. 77, No. 2.
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