Phenylarsine oxide inhibits tyrosine phosphorylation of phospholipase Cγ2 in human platelets and phospholipase Cγ1 in NIH-3T3 fibroblasts

Fumi Yanaga, Judith Asselin, Gary L. Schieven, Steve P. Watson

Research output: Contribution to journalArticle

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Abstract

The sulphydryl reagent phenylarsine oxide (PAO) (1 μM) inhibited completely formation of inositol phosphates in human platelets induced by collagen or by cross-linking of the platelet low affinity Fc receptor, FcγRIIA, but did not alter the response to the G protein receptor agonist thrombin. PAO also inhibited completely tyrosine phosphorylation of PLCγ2 in collagen and FcγRIIA-stimulated cells, although tyrosine phosphorylation of other proteins including the tyrosine kinase syk was relatively unaffected. PAO (1 μM) also inhibited completely tyrosine phosphorylation of PLCγ1 induced by platelet derived growth factor (PDGF) in NIH-3T3 fibroblasts but only partially reduced phosphorylation of the PDGF receptor. These results provide further evidence that collagen and FcγRIIA cross-linking activate platelets through a pathway distinct from that used by thrombin and suggest that PAO may be a selective inhibitor of PLCγ relative to PLC β isozymes.

Original languageEnglish
Pages (from-to)377-380
Number of pages4
JournalFEBS Letters
Volume368
Issue number2
DOIs
Publication statusPublished - Jul 17 1995

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Phosphorylation
Phospholipases
Fibroblasts
Platelets
Tyrosine
Blood Platelets
Collagen
Programmable logic controllers
Thrombin
Thrombin Receptors
Platelet-Derived Growth Factor Receptors
Inositol Phosphates
Fc Receptors
Platelet-Derived Growth Factor
GTP-Binding Proteins
Protein-Tyrosine Kinases
Isoenzymes
oxophenylarsine
phospholipase C1
Proteins

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Phenylarsine oxide inhibits tyrosine phosphorylation of phospholipase Cγ2 in human platelets and phospholipase Cγ1 in NIH-3T3 fibroblasts. / Yanaga, Fumi; Asselin, Judith; Schieven, Gary L.; Watson, Steve P.

In: FEBS Letters, Vol. 368, No. 2, 17.07.1995, p. 377-380.

Research output: Contribution to journalArticle

Yanaga, Fumi ; Asselin, Judith ; Schieven, Gary L. ; Watson, Steve P. / Phenylarsine oxide inhibits tyrosine phosphorylation of phospholipase Cγ2 in human platelets and phospholipase Cγ1 in NIH-3T3 fibroblasts. In: FEBS Letters. 1995 ; Vol. 368, No. 2. pp. 377-380.
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