Phorbol ester receptors and the induction of differentiation in the human t lymphoblastic cell line molt-3

Takehito Mayumi, Kohei Nagasawa, Takahiko Horiuchi

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2 Citations (Scopus)

Abstract

To examine the mechanism of induction of differentiation in the human malignant T-lymphoblastic cell line, MOLT-3, by 12-O-tetradecanoylphorbol-13-acetate (TPA), the role of receptors for phorbol esters was investigated. Binding of [20-3H]-phorbol-12, 13-dibutyrate to TPA-resistant subclones derived from MOLT-3 was less than 50% of that of the parental MOLT-3. Scatchard analysis showed that the concentration of phorbol ester receptors in a TPA-resistant subclone was about 50% of that in the parental MOLT-3, but affinities of binding were similar, indicating that more than a certain number of phorbol ester receptors is required to induce differentiation by TPA in this human T cell line.

Original languageEnglish
Pages (from-to)12-19
Number of pages8
JournalPathobiology
Volume56
Issue number1-2
DOIs
Publication statusPublished - Jan 1 1988

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

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