Phosphatidylcholine bearing 6,6-dideuterated oleic acid: A useful solid-state 2H NMR probe for investigating membrane properties

Jin Cui, Sébastien Lethu, Tomokazu Yasuda, Shigeru Matsuoka, Nobuaki Matsumori, Fuminori Sato, Michio Murata

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Lipid organization has been at the center of research on lipid rafts. Dioleoylphosphatidylcholine (DOPC) is a typical unsaturated lipid. Very few studies have reported its thermodynamics in raft-like membranes. Herein, we have developed a highly efficient synthetic method for [C6-2H2] oleic acid, and newly synthesized [C6-2H2] DOPC. In raft-like oriented bilayers, [C6-2H2] DOPC shows clear phase separation and characteristic phase behavior at various temperature. It has been successfully utilized for the comparison of membrane properties between sphingomyelin (SM) and dihydrosphingomyelin (DHSM) membranes.

Original languageEnglish
Pages (from-to)203-206
Number of pages4
JournalBioorganic and Medicinal Chemistry Letters
Volume25
Issue number2
DOIs
Publication statusPublished - Jan 15 2015

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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