Phosphatidylinositol 3-kinase encoded by yeast VPS34 gene essential for protein sorting

Peter V. Schu, Kaoru Takegawa, Michael J. Fry, Jeffrey H. Stack, Michael D. Waterfield, Scott D. Emr

Research output: Contribution to journalArticle

746 Citations (Scopus)

Abstract

The VPS34 gene product (Vps34p) is required for protein sorting to the lysosome-like vacuole of the yeast Saccharomyces cerevisiae. Vps34p shares significant sequence similarity with the catalytic subunit of bovine phosphatidylinositol (PI) 3-kinase [the 110-kilodalton (p110) subunit of PI 3-kinase], which is known to interact with activated cell surface receptor tyrosine kinases. Yeast strains deleted for the VPS34 gene or carrying vps34 point mutations lacked detectable PI 3-kinase activity and exhibited severe defects in vacuolar protein sorting. Overexpression of Vps34p resulted in an increase in PI 3-kinase activity, and this activity was specifically precipitated with antisera to Vps34p. VPS34 encodes a yeast PI 3-kinase, and this enzyme appears to regulate intracellular protein trafficking decisions.

Original languageEnglish
Pages (from-to)88-91
Number of pages4
JournalScience
Volume260
Issue number5104
DOIs
Publication statusPublished - Jan 1 1993
Externally publishedYes

Fingerprint

Phosphatidylinositol 3-Kinase
Protein Transport
Yeasts
Genes
Proteins
Cell Surface Receptors
Vacuoles
Lysosomes
Point Mutation
Protein-Tyrosine Kinases
Saccharomyces cerevisiae
Immune Sera
Catalytic Domain
Enzymes

All Science Journal Classification (ASJC) codes

  • General

Cite this

Schu, P. V., Takegawa, K., Fry, M. J., Stack, J. H., Waterfield, M. D., & Emr, S. D. (1993). Phosphatidylinositol 3-kinase encoded by yeast VPS34 gene essential for protein sorting. Science, 260(5104), 88-91. https://doi.org/10.1126/science.8385367

Phosphatidylinositol 3-kinase encoded by yeast VPS34 gene essential for protein sorting. / Schu, Peter V.; Takegawa, Kaoru; Fry, Michael J.; Stack, Jeffrey H.; Waterfield, Michael D.; Emr, Scott D.

In: Science, Vol. 260, No. 5104, 01.01.1993, p. 88-91.

Research output: Contribution to journalArticle

Schu, PV, Takegawa, K, Fry, MJ, Stack, JH, Waterfield, MD & Emr, SD 1993, 'Phosphatidylinositol 3-kinase encoded by yeast VPS34 gene essential for protein sorting', Science, vol. 260, no. 5104, pp. 88-91. https://doi.org/10.1126/science.8385367
Schu, Peter V. ; Takegawa, Kaoru ; Fry, Michael J. ; Stack, Jeffrey H. ; Waterfield, Michael D. ; Emr, Scott D. / Phosphatidylinositol 3-kinase encoded by yeast VPS34 gene essential for protein sorting. In: Science. 1993 ; Vol. 260, No. 5104. pp. 88-91.
@article{4d1a38828f4f429496c95b58e3b4d101,
title = "Phosphatidylinositol 3-kinase encoded by yeast VPS34 gene essential for protein sorting",
abstract = "The VPS34 gene product (Vps34p) is required for protein sorting to the lysosome-like vacuole of the yeast Saccharomyces cerevisiae. Vps34p shares significant sequence similarity with the catalytic subunit of bovine phosphatidylinositol (PI) 3-kinase [the 110-kilodalton (p110) subunit of PI 3-kinase], which is known to interact with activated cell surface receptor tyrosine kinases. Yeast strains deleted for the VPS34 gene or carrying vps34 point mutations lacked detectable PI 3-kinase activity and exhibited severe defects in vacuolar protein sorting. Overexpression of Vps34p resulted in an increase in PI 3-kinase activity, and this activity was specifically precipitated with antisera to Vps34p. VPS34 encodes a yeast PI 3-kinase, and this enzyme appears to regulate intracellular protein trafficking decisions.",
author = "Schu, {Peter V.} and Kaoru Takegawa and Fry, {Michael J.} and Stack, {Jeffrey H.} and Waterfield, {Michael D.} and Emr, {Scott D.}",
year = "1993",
month = "1",
day = "1",
doi = "10.1126/science.8385367",
language = "English",
volume = "260",
pages = "88--91",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "5104",

}

TY - JOUR

T1 - Phosphatidylinositol 3-kinase encoded by yeast VPS34 gene essential for protein sorting

AU - Schu, Peter V.

AU - Takegawa, Kaoru

AU - Fry, Michael J.

AU - Stack, Jeffrey H.

AU - Waterfield, Michael D.

AU - Emr, Scott D.

PY - 1993/1/1

Y1 - 1993/1/1

N2 - The VPS34 gene product (Vps34p) is required for protein sorting to the lysosome-like vacuole of the yeast Saccharomyces cerevisiae. Vps34p shares significant sequence similarity with the catalytic subunit of bovine phosphatidylinositol (PI) 3-kinase [the 110-kilodalton (p110) subunit of PI 3-kinase], which is known to interact with activated cell surface receptor tyrosine kinases. Yeast strains deleted for the VPS34 gene or carrying vps34 point mutations lacked detectable PI 3-kinase activity and exhibited severe defects in vacuolar protein sorting. Overexpression of Vps34p resulted in an increase in PI 3-kinase activity, and this activity was specifically precipitated with antisera to Vps34p. VPS34 encodes a yeast PI 3-kinase, and this enzyme appears to regulate intracellular protein trafficking decisions.

AB - The VPS34 gene product (Vps34p) is required for protein sorting to the lysosome-like vacuole of the yeast Saccharomyces cerevisiae. Vps34p shares significant sequence similarity with the catalytic subunit of bovine phosphatidylinositol (PI) 3-kinase [the 110-kilodalton (p110) subunit of PI 3-kinase], which is known to interact with activated cell surface receptor tyrosine kinases. Yeast strains deleted for the VPS34 gene or carrying vps34 point mutations lacked detectable PI 3-kinase activity and exhibited severe defects in vacuolar protein sorting. Overexpression of Vps34p resulted in an increase in PI 3-kinase activity, and this activity was specifically precipitated with antisera to Vps34p. VPS34 encodes a yeast PI 3-kinase, and this enzyme appears to regulate intracellular protein trafficking decisions.

UR - http://www.scopus.com/inward/record.url?scp=0027905021&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027905021&partnerID=8YFLogxK

U2 - 10.1126/science.8385367

DO - 10.1126/science.8385367

M3 - Article

C2 - 8385367

AN - SCOPUS:0027905021

VL - 260

SP - 88

EP - 91

JO - Science

JF - Science

SN - 0036-8075

IS - 5104

ER -