Phosphatidylinositol 3-kinase encoded by yeast VPS34 gene essential for protein sorting

Peter V. Schu, Kaoru Takegawa, Michael J. Fry, Jeffrey H. Stack, Michael D. Waterfield, Scott D. Emr

Research output: Contribution to journalArticlepeer-review

766 Citations (Scopus)

Abstract

The VPS34 gene product (Vps34p) is required for protein sorting to the lysosome-like vacuole of the yeast Saccharomyces cerevisiae. Vps34p shares significant sequence similarity with the catalytic subunit of bovine phosphatidylinositol (PI) 3-kinase [the 110-kilodalton (p110) subunit of PI 3-kinase], which is known to interact with activated cell surface receptor tyrosine kinases. Yeast strains deleted for the VPS34 gene or carrying vps34 point mutations lacked detectable PI 3-kinase activity and exhibited severe defects in vacuolar protein sorting. Overexpression of Vps34p resulted in an increase in PI 3-kinase activity, and this activity was specifically precipitated with antisera to Vps34p. VPS34 encodes a yeast PI 3-kinase, and this enzyme appears to regulate intracellular protein trafficking decisions.

Original languageEnglish
Pages (from-to)88-91
Number of pages4
JournalScience
Volume260
Issue number5104
DOIs
Publication statusPublished - Jan 1 1993
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General

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