Abstract
The VPS34 gene product (Vps34p) is required for protein sorting to the lysosome-like vacuole of the yeast Saccharomyces cerevisiae. Vps34p shares significant sequence similarity with the catalytic subunit of bovine phosphatidylinositol (PI) 3-kinase [the 110-kilodalton (p110) subunit of PI 3-kinase], which is known to interact with activated cell surface receptor tyrosine kinases. Yeast strains deleted for the VPS34 gene or carrying vps34 point mutations lacked detectable PI 3-kinase activity and exhibited severe defects in vacuolar protein sorting. Overexpression of Vps34p resulted in an increase in PI 3-kinase activity, and this activity was specifically precipitated with antisera to Vps34p. VPS34 encodes a yeast PI 3-kinase, and this enzyme appears to regulate intracellular protein trafficking decisions.
| Original language | English |
|---|---|
| Pages (from-to) | 88-91 |
| Number of pages | 4 |
| Journal | Science |
| Volume | 260 |
| Issue number | 5104 |
| DOIs | |
| Publication status | Published - Jan 1 1993 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- General
Cite this
Phosphatidylinositol 3-kinase encoded by yeast VPS34 gene essential for protein sorting. / Schu, Peter V.; Takegawa, Kaoru; Fry, Michael J.; Stack, Jeffrey H.; Waterfield, Michael D.; Emr, Scott D.
In: Science, Vol. 260, No. 5104, 01.01.1993, p. 88-91.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Phosphatidylinositol 3-kinase encoded by yeast VPS34 gene essential for protein sorting
AU - Schu, Peter V.
AU - Takegawa, Kaoru
AU - Fry, Michael J.
AU - Stack, Jeffrey H.
AU - Waterfield, Michael D.
AU - Emr, Scott D.
PY - 1993/1/1
Y1 - 1993/1/1
N2 - The VPS34 gene product (Vps34p) is required for protein sorting to the lysosome-like vacuole of the yeast Saccharomyces cerevisiae. Vps34p shares significant sequence similarity with the catalytic subunit of bovine phosphatidylinositol (PI) 3-kinase [the 110-kilodalton (p110) subunit of PI 3-kinase], which is known to interact with activated cell surface receptor tyrosine kinases. Yeast strains deleted for the VPS34 gene or carrying vps34 point mutations lacked detectable PI 3-kinase activity and exhibited severe defects in vacuolar protein sorting. Overexpression of Vps34p resulted in an increase in PI 3-kinase activity, and this activity was specifically precipitated with antisera to Vps34p. VPS34 encodes a yeast PI 3-kinase, and this enzyme appears to regulate intracellular protein trafficking decisions.
AB - The VPS34 gene product (Vps34p) is required for protein sorting to the lysosome-like vacuole of the yeast Saccharomyces cerevisiae. Vps34p shares significant sequence similarity with the catalytic subunit of bovine phosphatidylinositol (PI) 3-kinase [the 110-kilodalton (p110) subunit of PI 3-kinase], which is known to interact with activated cell surface receptor tyrosine kinases. Yeast strains deleted for the VPS34 gene or carrying vps34 point mutations lacked detectable PI 3-kinase activity and exhibited severe defects in vacuolar protein sorting. Overexpression of Vps34p resulted in an increase in PI 3-kinase activity, and this activity was specifically precipitated with antisera to Vps34p. VPS34 encodes a yeast PI 3-kinase, and this enzyme appears to regulate intracellular protein trafficking decisions.
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UR - http://www.scopus.com/inward/citedby.url?scp=0027905021&partnerID=8YFLogxK
U2 - 10.1126/science.8385367
DO - 10.1126/science.8385367
M3 - Article
C2 - 8385367
AN - SCOPUS:0027905021
VL - 260
SP - 88
EP - 91
JO - Science
JF - Science
SN - 0036-8075
IS - 5104
ER -