Phosphatidylinositol 3-phosphate 5-kinase is required for the cellular response to nutritional starvation and mating pheromone signals in Schizosaccharomyces pombe

Masayo Morishita, Fusako Morimoto, Kenji Kitamura, Takako Koga, Yasuhisa Fukui, Hiromi Maekawa, Ichiro Yamashita, Chikashi Shimoda

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Background: Phosphatidylinositol (3,5) bisphosphate, which is converted from phosphatidylinositol 3-phosphate by phosphatidylinositol 3-phosphate 5-kinase, is implicated in vacuolar functions and the sorting of cell surface proteins within endosomes in the endocytic pathway of budding yeast. A homologous protein, SpFab1p, has been found in the fission yeast Schizosaccharomyces pombe, but its role is not known. Results: Here we report that SpFab1p is encoded by ste12+ known as a fertility gene in S. pombe. The ste12 mutant grew normally under stress-free conditions, but was highly vacuolated and swelled at high temperatures and under starvation conditions. In nitrogen-free medium, ste12 cells were arrested in G1 phase, but partially defective in the expression of genes responsible for mating and meiosis. The ste12 mutant was defective both in the production of, and in the response to, mating pheromones. The amount of the pheromone receptor protein Map3p, was substantially decreased in ste12 cells. Map3p was transported to the cell surface, then internalized and eventually transported to the vacuolar lumen, even in the ste12 mutant. Conclusion: The results indicate that phosphatidylinositol(3,5)bisphosphate is essential for cellular responses to various stresses and for the mating pheromone signalling under starvation conditions.

Original languageEnglish
Pages (from-to)199-215
Number of pages17
JournalGenes to Cells
Volume7
Issue number2
DOIs
Publication statusPublished - Mar 21 2002
Externally publishedYes

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Schizosaccharomyces
Pheromones
Starvation
Phosphotransferases
Pheromone Receptors
Saccharomycetales
Endosomes
Meiosis
G1 Phase
Fertility
Membrane Proteins
Proteins
Nitrogen
Gene Expression
Temperature
Genes
phosphatidylinositol 3-phosphate
phosphatidylinositol 3,5-diphosphate

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

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Phosphatidylinositol 3-phosphate 5-kinase is required for the cellular response to nutritional starvation and mating pheromone signals in Schizosaccharomyces pombe. / Morishita, Masayo; Morimoto, Fusako; Kitamura, Kenji; Koga, Takako; Fukui, Yasuhisa; Maekawa, Hiromi; Yamashita, Ichiro; Shimoda, Chikashi.

In: Genes to Cells, Vol. 7, No. 2, 21.03.2002, p. 199-215.

Research output: Contribution to journalArticle

Morishita, Masayo ; Morimoto, Fusako ; Kitamura, Kenji ; Koga, Takako ; Fukui, Yasuhisa ; Maekawa, Hiromi ; Yamashita, Ichiro ; Shimoda, Chikashi. / Phosphatidylinositol 3-phosphate 5-kinase is required for the cellular response to nutritional starvation and mating pheromone signals in Schizosaccharomyces pombe. In: Genes to Cells. 2002 ; Vol. 7, No. 2. pp. 199-215.
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