Abstract
Sindbis virus replication is mediated by an RNA replicase translated from viral RNA genome. The replicase synthesizes progeny genomic RNA and shorter RNA (subgenomic RNA) carrying viral structural genes in association with cytoplasmic membranes. Here we examined the involvement of a membrane lipid, phosphatidylserine (PS), in Sindbis virus gene expression using Chinese hamster ovary cell mutants. When the mutant cells were transfected with viral replicon RNA, in which the structural genes downstream of the subgenomic promoter were replaced by a reporter gene, reporter expression was inhibited under PS-deficient conditions. In contrast, reporter expression from an SV40 promoter-driven construct was normal under similar conditions. Furthermore, expression of a viral replicase protein from the genomic RNA and accumulation of the subgenomic RNA were not inhibited by PS deficiency. These findings indicate that reduced cellular PS level impairs a posttranscriptional event of Sindbis virus subgenomic promoter-driven gene expression.
| Original language | English |
|---|---|
| Pages (from-to) | 878-885 |
| Number of pages | 8 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 345 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jun 30 2006 |
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All Science Journal Classification (ASJC) codes
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Phosphatidylserine is involved in gene expression from Sindbis virus subgenomic promoter. / Saito, Kyoko; Nishijima, Masahiro; Kuge, Osamu.
In: Biochemical and Biophysical Research Communications, Vol. 345, No. 2, 30.06.2006, p. 878-885.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Phosphatidylserine is involved in gene expression from Sindbis virus subgenomic promoter
AU - Saito, Kyoko
AU - Nishijima, Masahiro
AU - Kuge, Osamu
PY - 2006/6/30
Y1 - 2006/6/30
N2 - Sindbis virus replication is mediated by an RNA replicase translated from viral RNA genome. The replicase synthesizes progeny genomic RNA and shorter RNA (subgenomic RNA) carrying viral structural genes in association with cytoplasmic membranes. Here we examined the involvement of a membrane lipid, phosphatidylserine (PS), in Sindbis virus gene expression using Chinese hamster ovary cell mutants. When the mutant cells were transfected with viral replicon RNA, in which the structural genes downstream of the subgenomic promoter were replaced by a reporter gene, reporter expression was inhibited under PS-deficient conditions. In contrast, reporter expression from an SV40 promoter-driven construct was normal under similar conditions. Furthermore, expression of a viral replicase protein from the genomic RNA and accumulation of the subgenomic RNA were not inhibited by PS deficiency. These findings indicate that reduced cellular PS level impairs a posttranscriptional event of Sindbis virus subgenomic promoter-driven gene expression.
AB - Sindbis virus replication is mediated by an RNA replicase translated from viral RNA genome. The replicase synthesizes progeny genomic RNA and shorter RNA (subgenomic RNA) carrying viral structural genes in association with cytoplasmic membranes. Here we examined the involvement of a membrane lipid, phosphatidylserine (PS), in Sindbis virus gene expression using Chinese hamster ovary cell mutants. When the mutant cells were transfected with viral replicon RNA, in which the structural genes downstream of the subgenomic promoter were replaced by a reporter gene, reporter expression was inhibited under PS-deficient conditions. In contrast, reporter expression from an SV40 promoter-driven construct was normal under similar conditions. Furthermore, expression of a viral replicase protein from the genomic RNA and accumulation of the subgenomic RNA were not inhibited by PS deficiency. These findings indicate that reduced cellular PS level impairs a posttranscriptional event of Sindbis virus subgenomic promoter-driven gene expression.
UR - http://www.scopus.com/inward/record.url?scp=33646842011&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33646842011&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2006.04.138
DO - 10.1016/j.bbrc.2006.04.138
M3 - Article
C2 - 16701551
AN - SCOPUS:33646842011
VL - 345
SP - 878
EP - 885
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -