Phospholipase C-related catalytically inactive protein, a novel microtubule-associated protein 1 light chain 3-binding protein, negatively regulates autophagosome formation

Hisanori Umebayashi, Akiko Mizokami, Miho Matsuda, Kae Harada, Hiroshi Takeuchi, Isei Tanida, Masato Hirata, Takashi Kanematsu

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Upon starvation, cells undergo autophagy, an intracellular bulk-degradation process, to provide the required nutrients. Here, we observed that phospholipase C-related catalytically inactive protein (PRIP) binds to microtubule-associated protein 1 light chain 3 (LC3), a mammalian autophagy-related initiator that regulates the autophagy pathway. Then, we examined the involvement of PRIP in the nutrient depletion-induced autophagy pathway. Enhanced colocalization of PRIP with LC3 was clearly seen in nutrient-starved mouse embryonic fibroblasts under a fluorescent microscope, and interaction of the proteins was revealed by immunoprecipitation experiments with an anti-LC3 antibody. Under starvation conditions, there were more green fluorescent protein fused-LC3 dots in mouse embryonic fibroblasts from PRIP-deficient mice than in fibroblasts from wild type cells. The formation of new dots in a single cell increased, as assessed by time-lapse microscopy. Furthermore, the increase in autophagosome formation in PRIP-deficient cells was notably inhibited by exogenously overexpressed PRIP. Taken together, PRIP is a novel LC3-binding protein that acts as a negative modulator of autophagosome formation.

Original languageEnglish
Pages (from-to)268-274
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume432
Issue number2
DOIs
Publication statusPublished - Mar 8 2013

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Microtubule-Associated Proteins
Type C Phospholipases
Carrier Proteins
Light
Autophagy
Proteins
Fibroblasts
Nutrients
Starvation
Food
Autophagosomes
Green Fluorescent Proteins
Immunoprecipitation
Modulators
Microscopy
Microscopic examination
Microscopes
Degradation
Antibodies

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Phospholipase C-related catalytically inactive protein, a novel microtubule-associated protein 1 light chain 3-binding protein, negatively regulates autophagosome formation. / Umebayashi, Hisanori; Mizokami, Akiko; Matsuda, Miho; Harada, Kae; Takeuchi, Hiroshi; Tanida, Isei; Hirata, Masato; Kanematsu, Takashi.

In: Biochemical and Biophysical Research Communications, Vol. 432, No. 2, 08.03.2013, p. 268-274.

Research output: Contribution to journalArticle

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