Phospholipase C-related catalytically inactive protein (PRIP) controls KIF5B-mediated insulin secretion

Satoshi Asano, Tomomi Nemoto, Tomoya Kitayama, Kae Harada, Jun Zhang, Kana Harada, Isei Tanida, Masato Hirata, Takashi Kanematsu

Research output: Contribution to journalArticle

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Abstract

We previously reported that phospholipase C-related catalytically inactive protein (PRIP)-knockout mice exhibited hyperinsulinemia. Here, we investigated the role of PRIP in insulin granule exocytosis using Prip-knockdown mouse insulinoma (MIN6) cells. Insulin release from Prip-knockdown MIN6 cells was higher than that from control cells, and Prip knockdown facilitated movement of GFP-phogrinlabeled insulin secretory vesicles. Double-immunofluorescent staining and density step-gradient analyses showed that the KIF5B motor protein co-localized with insulin vesicles in Prip-knockdown MIN6 cells. Knockdown of GABAA -receptor-associated protein (GABARAP), a microtubule-associated PRIP-binding partner, by Gabarap silencing in MIN6 cells reduced the co-localization of insulin vesicles with KIF5B and the movement of vesicles, resulting in decreased insulin secretion. However, the co-localization of KIF5B with microtubules was not altered in Prip- and Gabarap-knockdown cells. The presence of unbound GABARAP, freed either by an interference peptide or by Prip silencing, in MIN6 cells enhanced the co-localization of insulin vesicles with microtubules and promoted vesicle mobility. Taken together, these data demonstrate that PRIP and GABARAP function in a complex to regulate KIF5B-mediated insulin secretion, providing new insights into insulin exocytic mechanisms.

Original languageEnglish
Pages (from-to)463-474
Number of pages12
JournalBiology Open
Volume3
Issue number6
DOIs
Publication statusPublished - Jun 15 2014

Fingerprint

phospholipase C
insulin secretion
Type C Phospholipases
insulin
Insulin
Proteins
microtubules
proteins
cells
GABA-A Receptors
Microtubule-Associated Proteins
receptors
Microtubules
hyperinsulinemia
exocytosis
secretory granules
protein binding
mice
Insulinoma
granules

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Phospholipase C-related catalytically inactive protein (PRIP) controls KIF5B-mediated insulin secretion. / Asano, Satoshi; Nemoto, Tomomi; Kitayama, Tomoya; Harada, Kae; Zhang, Jun; Harada, Kana; Tanida, Isei; Hirata, Masato; Kanematsu, Takashi.

In: Biology Open, Vol. 3, No. 6, 15.06.2014, p. 463-474.

Research output: Contribution to journalArticle

Asano, S, Nemoto, T, Kitayama, T, Harada, K, Zhang, J, Harada, K, Tanida, I, Hirata, M & Kanematsu, T 2014, 'Phospholipase C-related catalytically inactive protein (PRIP) controls KIF5B-mediated insulin secretion', Biology Open, vol. 3, no. 6, pp. 463-474. https://doi.org/10.1242/bio.20147591
Asano, Satoshi ; Nemoto, Tomomi ; Kitayama, Tomoya ; Harada, Kae ; Zhang, Jun ; Harada, Kana ; Tanida, Isei ; Hirata, Masato ; Kanematsu, Takashi. / Phospholipase C-related catalytically inactive protein (PRIP) controls KIF5B-mediated insulin secretion. In: Biology Open. 2014 ; Vol. 3, No. 6. pp. 463-474.
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