Phospholipase C-related catalytically inactive protein regulates cytokinesis by protecting phosphatidylinositol 4,5-bisphosphate from metabolism in the cleavage furrow

Satoshi Asano, Yasuka Ikura, Mitsuki Nishimoto, Yosuke Yamawaki, Kozue Hamao, Keiju Kamijo, Masato Hirata, Takashi Kanematsu

Research output: Contribution to journalArticle

Abstract

Cytokinesis is initiated by the formation and ingression of the cleavage furrow. Phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] accumulation followed by RhoA translocation to the cleavage furrow are prerequisites for cytokinesis progression. Here, we investigated whether phospholipase C (PLC)-related catalytically inactive protein (PRIP), a metabolic modulator of PI(4,5)P2, regulates PI(4,5)P2-mediated cytokinesis. We found that PRIP localised to the cleavage furrow during cytokinesis. Moreover, HeLa cells with silenced PRIP displayed abnormal cytokinesis. Importantly, PI(4,5)P2 accumulation at the cleavage furrow, as well as the localisation of RhoA and phospho-myosin II regulatory light chain to the cleavage furrow, were reduced in PRIP-silenced cells. The overexpression of oculocerebrorenal syndrome of Lowe-1 (OCRL1), a phosphatidylinositol-5-phosphatase, in cells decreased PI(4,5)P2 levels during early cytokinesis and resulted in cytokinesis abnormalities. However, these abnormal cytokinesis phenotypes were ameliorated by the co-expression of PRIP but not by co-expression of a PI(4,5)P2-unbound PRIP mutant. Collectively, our results indicate that PRIP is a component at the cleavage furrow that maintains PI(4,5)P2 metabolism and regulates RhoA-dependent progression of cytokinesis. Thus, we propose that PRIP regulates phosphoinositide metabolism correctively and mediates normal cytokinesis progression.

Original languageEnglish
Article number12729
JournalScientific reports
Volume9
Issue number1
DOIs
Publication statusPublished - Dec 1 2019

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Cytokinesis
Type C Phospholipases
Phosphatidylinositols
Proteins
Oculocerebrorenal Syndrome
Myosin Type II
Myosin Light Chains
Mutant Proteins
HeLa Cells
Phosphoric Monoester Hydrolases
Phenotype

All Science Journal Classification (ASJC) codes

  • General

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Phospholipase C-related catalytically inactive protein regulates cytokinesis by protecting phosphatidylinositol 4,5-bisphosphate from metabolism in the cleavage furrow. / Asano, Satoshi; Ikura, Yasuka; Nishimoto, Mitsuki; Yamawaki, Yosuke; Hamao, Kozue; Kamijo, Keiju; Hirata, Masato; Kanematsu, Takashi.

In: Scientific reports, Vol. 9, No. 1, 12729, 01.12.2019.

Research output: Contribution to journalArticle

Asano, Satoshi ; Ikura, Yasuka ; Nishimoto, Mitsuki ; Yamawaki, Yosuke ; Hamao, Kozue ; Kamijo, Keiju ; Hirata, Masato ; Kanematsu, Takashi. / Phospholipase C-related catalytically inactive protein regulates cytokinesis by protecting phosphatidylinositol 4,5-bisphosphate from metabolism in the cleavage furrow. In: Scientific reports. 2019 ; Vol. 9, No. 1.
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AU - Hamao, Kozue

AU - Kamijo, Keiju

AU - Hirata, Masato

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