Phosphorylation of EB2 by Aurora B and CDK1 ensures mitotic progression and genome stability

Makoto Iimori, Sugiko Watanabe, Shinichi Kiyonari, Kazuaki Matsuoka, Ryo Sakasai, Hiroshi Saeki, Eiji Oki, Hiroyuki Kitao, Yoshihiko Maehara

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Temporal regulation of microtubule dynamics is essential for proper progression of mitosis and control of microtubule plus-end tracking proteins by phosphorylation is an essential component of this regulation. Here we show that Aurora B and CDK1 phosphorylate microtubule end-binding protein 2 (EB2) at multiple sites within the amino terminus and a cluster of serine/threonine residues in the linker connecting the calponin homology and end-binding homology domains. EB2 phosphorylation, which is strictly associated with mitotic entry and progression, reduces the binding affinity of EB2 for microtubules. Expression of non-phosphorylatable EB2 induces stable kinetochore microtubule dynamics and delays formation of bipolar metaphase plates in a microtubule binding-dependent manner, and leads to aneuploidy even in unperturbed mitosis. We propose that Aurora B and CDK1 temporally regulate the binding affinity of EB2 for microtubules, thereby ensuring kinetochore microtubule dynamics, proper mitotic progression and genome stability.

Original languageEnglish
Article number11117
JournalNature communications
Volume7
DOIs
Publication statusPublished - Mar 31 2016

Fingerprint

phosphorylation
Phosphorylation
genome
Genomic Instability
progressions
Microtubules
Carrier Proteins
Genes
proteins
Kinetochores
mitosis
homology
Mitosis
Threonine
affinity
Serine
Aneuploidy
Metaphase
entry
Proteins

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Iimori, M., Watanabe, S., Kiyonari, S., Matsuoka, K., Sakasai, R., Saeki, H., ... Maehara, Y. (2016). Phosphorylation of EB2 by Aurora B and CDK1 ensures mitotic progression and genome stability. Nature communications, 7, [11117]. https://doi.org/10.1038/ncomms11117

Phosphorylation of EB2 by Aurora B and CDK1 ensures mitotic progression and genome stability. / Iimori, Makoto; Watanabe, Sugiko; Kiyonari, Shinichi; Matsuoka, Kazuaki; Sakasai, Ryo; Saeki, Hiroshi; Oki, Eiji; Kitao, Hiroyuki; Maehara, Yoshihiko.

In: Nature communications, Vol. 7, 11117, 31.03.2016.

Research output: Contribution to journalArticle

Iimori, Makoto ; Watanabe, Sugiko ; Kiyonari, Shinichi ; Matsuoka, Kazuaki ; Sakasai, Ryo ; Saeki, Hiroshi ; Oki, Eiji ; Kitao, Hiroyuki ; Maehara, Yoshihiko. / Phosphorylation of EB2 by Aurora B and CDK1 ensures mitotic progression and genome stability. In: Nature communications. 2016 ; Vol. 7.
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