Phosphorylation of mitogen-activated protein kinase cascade during early embryo development in the mouse

N. Iwamori, K. Naito, K. Sugiura, H. Kagii, M. Yamashita, S. Ohashi, S. Goto, K. Yamanouchi, H. Tojo

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The mitogen-activated protein kinase (MAPK) cascade is one of the most important signal transduction pathways that regulate the cell cycle in somatic cells. The present study examined the phosphorylation states of components in the MAPK cascade, Raf-1, MEK-1, and extracellular signal regulated kinases (ERKs), which are activated by mitogens, throughout early mouse embryo development and in cultured somatic cells generally. In somatic cells, Raf-1 and MEK-1 were phosphorylated at M-phase and dephosphorylated during interphase. ERKs were not phosphorylated at any stage during the cell cycle. These results were similar to previous findings for the first and second cell cycles of early mouse embryos. In contrast, after the four-cell stage, not only ERKs, but also Raf-1 and MEK-1, were not phosphorylated at any stage during the cell cycle in mouse early embryos. These results suggest that the MAPK cascade in mouse-embryos is regulated by the same mechanism as in somatic cells before the two-cell stage, and that regulation is changed to an embryo-specific mechanism after the four-cell stage.

Original languageEnglish
Pages (from-to)209-214
Number of pages6
JournalReproduction, Fertility and Development
Volume12
Issue number3-4
Publication statusPublished - Dec 1 2000
Externally publishedYes

Fingerprint

Mitogen-Activated Protein Kinases
mitogen-activated protein kinase
Embryonic Development
phosphorylation
embryogenesis
Phosphorylation
somatic cells
cell cycle
Mitogen-Activated Protein Kinase Kinases
Cell Cycle
mice
embryo (animal)
Embryonic Structures
Proto-Oncogene Proteins c-raf
Extracellular Signal-Regulated MAP Kinases
Mitogen-Activated Protein Kinase 3
Interphase
cells
Mitogens
interphase

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Reproductive Medicine
  • Animal Science and Zoology
  • Molecular Biology
  • Genetics
  • Endocrinology
  • Developmental Biology

Cite this

Iwamori, N., Naito, K., Sugiura, K., Kagii, H., Yamashita, M., Ohashi, S., ... Tojo, H. (2000). Phosphorylation of mitogen-activated protein kinase cascade during early embryo development in the mouse. Reproduction, Fertility and Development, 12(3-4), 209-214.

Phosphorylation of mitogen-activated protein kinase cascade during early embryo development in the mouse. / Iwamori, N.; Naito, K.; Sugiura, K.; Kagii, H.; Yamashita, M.; Ohashi, S.; Goto, S.; Yamanouchi, K.; Tojo, H.

In: Reproduction, Fertility and Development, Vol. 12, No. 3-4, 01.12.2000, p. 209-214.

Research output: Contribution to journalArticle

Iwamori, N, Naito, K, Sugiura, K, Kagii, H, Yamashita, M, Ohashi, S, Goto, S, Yamanouchi, K & Tojo, H 2000, 'Phosphorylation of mitogen-activated protein kinase cascade during early embryo development in the mouse', Reproduction, Fertility and Development, vol. 12, no. 3-4, pp. 209-214.
Iwamori, N. ; Naito, K. ; Sugiura, K. ; Kagii, H. ; Yamashita, M. ; Ohashi, S. ; Goto, S. ; Yamanouchi, K. ; Tojo, H. / Phosphorylation of mitogen-activated protein kinase cascade during early embryo development in the mouse. In: Reproduction, Fertility and Development. 2000 ; Vol. 12, No. 3-4. pp. 209-214.
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