Phosphorylation of rabphilin-3A by calmodulin-dependent protein kinase II

Masaki Kato, Takuya Sasaki, Katsunori Imazumi, Kazuo Takahashi, Keishi Araki, Hiromichi Shirataki, Yoshiharu Matsuura, Atsuhiko Ishida, Hitoshi Fujisawa, Yoshimi Takai

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27 Citations (Scopus)

Abstract

Rabphilin-3A is a putative target protein for Rab3A small GTP-binding protein which is implicated in neurotransmitter release. We have examined here whether Rabphilin-3A is phosphorylated by calmodulin-dependent protein kinase II (CaMKII). Recombinant Rabphilin-3A was phosphorylated by CaMKII purified from rat brain. Two moles of phosphate were maximally incorporated into one mole of Rabphilin-3A. The phosphorylation sites were Ser34, Thr205, Thr209, and Thr537. These results suggest that the CaMKII-catalyzed phosphorylation of Rabphilin-3A may modulate neurotransmitter release.

Original languageEnglish
Pages (from-to)1776-1784
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume205
Issue number3
DOIs
Publication statusPublished - Dec 30 1994
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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