TY - JOUR
T1 - Phosphorylation of rabphilin-3A by calmodulin-dependent protein kinase II
AU - Kato, Masaki
AU - Sasaki, Takuya
AU - Imazumi, Katsunori
AU - Takahashi, Kazuo
AU - Araki, Keishi
AU - Shirataki, Hiromichi
AU - Matsuura, Yoshiharu
AU - Ishida, Atsuhiko
AU - Fujisawa, Hitoshi
AU - Takai, Yoshimi
PY - 1994/12/30
Y1 - 1994/12/30
N2 - Rabphilin-3A is a putative target protein for Rab3A small GTP-binding protein which is implicated in neurotransmitter release. We have examined here whether Rabphilin-3A is phosphorylated by calmodulin-dependent protein kinase II (CaMKII). Recombinant Rabphilin-3A was phosphorylated by CaMKII purified from rat brain. Two moles of phosphate were maximally incorporated into one mole of Rabphilin-3A. The phosphorylation sites were Ser34, Thr205, Thr209, and Thr537. These results suggest that the CaMKII-catalyzed phosphorylation of Rabphilin-3A may modulate neurotransmitter release.
AB - Rabphilin-3A is a putative target protein for Rab3A small GTP-binding protein which is implicated in neurotransmitter release. We have examined here whether Rabphilin-3A is phosphorylated by calmodulin-dependent protein kinase II (CaMKII). Recombinant Rabphilin-3A was phosphorylated by CaMKII purified from rat brain. Two moles of phosphate were maximally incorporated into one mole of Rabphilin-3A. The phosphorylation sites were Ser34, Thr205, Thr209, and Thr537. These results suggest that the CaMKII-catalyzed phosphorylation of Rabphilin-3A may modulate neurotransmitter release.
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U2 - 10.1006/bbrc.1994.2875
DO - 10.1006/bbrc.1994.2875
M3 - Article
C2 - 7811264
AN - SCOPUS:0028670205
SN - 0006-291X
VL - 205
SP - 1776
EP - 1784
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -