Phosphorylation of serine 114 on Atg32 mediates mitophagy

Yoshimasa Aoki, Tomotake Kanki, Yuko Hirota, Yusuke Kurihara, Tetsu Saigusa, Takeshi Uchiumi, Dongchon Kang

Research output: Contribution to journalArticle

117 Citations (Scopus)

Abstract

Mitophagy, which selectively degrades mitochondria via autophagy, has a significant role in mitochondrial quality control. When mitophagy is induced in yeast, mitochondrial residential protein Atg32 binds Atg11, an adaptor protein for selective types of autophagy, and it is recruited into the vacuole along with mitochondria. The Atg11-Atg32 interaction is believed to be the initial molecular step in which the autophagic machinery recognizes mitochondria as a cargo, although how this interaction is mediated is poorly understood. Therefore, we studied the Atg11-Atg32 interaction in detail. We found that the C-terminus region of Atg11, which included the fourth coiled-coil domain, interacted with the N-terminus region of Atg32 (residues 100-120). When mitophagy was induced, Ser-114 and Ser-119 on Atg32 were phosphorylated, and then the phosphorylation of Atg32, especially phosphorylation of Ser-114 on Atg32, mediated the Atg11-Atg32 interaction and mitophagy. These findings suggest that cells can regulate the amount of mitochondria, or select specific mitochondria (damaged or aged) that are degraded by mitophagy, by controlling the activity and/or localization of the kinase that phosphorylates Atg32. We also found that Hog1 and Pbs2, which are involved in the osmoregulatory signal transduction cascade, are related to Atg32 phosphorylation and mitophagy.

Original languageEnglish
Pages (from-to)3206-3217
Number of pages12
JournalMolecular biology of the cell
Volume22
Issue number17
DOIs
Publication statusPublished - Sep 1 2011

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Mitochondrial Degradation
Serine
Phosphorylation
Mitochondria
Autophagy
Mitochondrial Proteins
Vacuoles
Quality Control
Signal Transduction
Phosphotransferases
Yeasts

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

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Phosphorylation of serine 114 on Atg32 mediates mitophagy. / Aoki, Yoshimasa; Kanki, Tomotake; Hirota, Yuko; Kurihara, Yusuke; Saigusa, Tetsu; Uchiumi, Takeshi; Kang, Dongchon.

In: Molecular biology of the cell, Vol. 22, No. 17, 01.09.2011, p. 3206-3217.

Research output: Contribution to journalArticle

Aoki, Yoshimasa ; Kanki, Tomotake ; Hirota, Yuko ; Kurihara, Yusuke ; Saigusa, Tetsu ; Uchiumi, Takeshi ; Kang, Dongchon. / Phosphorylation of serine 114 on Atg32 mediates mitophagy. In: Molecular biology of the cell. 2011 ; Vol. 22, No. 17. pp. 3206-3217.
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