Phosphorylation of the p34cdc2 target site on goldfish germinal vesicle lamin B3 before oocyte maturation

Akihiko Yamaguchi, Yoshinao Katsu, Michiya Matsuyama, Michiyasu Yoshikuni, Yoshitaka Nagahama

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The nuclear membranes surrounding fish and frog oocyte germinal vesicles (GVs) are supported by the lamina, an internal, mesh-like structure that consists of the protein lamin B3. The mechanisms by which lamin B3 is transported into GVs and is assembled to form the nuclear lamina are not well understood. In this study, we developed a heterogeneous microinjection system in which wild-type or mutated goldfish GV lamin B3 (GFLB3) was expressed in Escherichia coli, biotinylated, and microinjected into Xenopus oocytes. The localization of the biotinylated GFLB3 was visualized by fluorescence confocal microscopy. The results of these experiments indicated that the N-terminal domain plays important roles in both nuclear transport and assembly of lamin B3 to form the nuclear lamina. The N-terminal domain includes a major consensus phosphoacceptor site for the p34cdc2 kinase at amino acid residue Ser-28. To investigate nuclear lamin phosphorylation, we generated a monoclonal antibody (C7B8D) against Ser-28-phosphorylated GFLB3. Two-dimensional (2-D) electrophoresis of GV protein revealed two major spots of lamin B3 with different isoelectric points (5.9 and 6.1). The C7B8D antibody recognized the pI-5.9 spot but not the pI-6.1 spot. The former spot disappeared when the native lamina was incubated with lambda phage protein phosphatase (λ-PP), indicating that a portion of the lamin protein was already phosphorylated in the goldfish GV-stage oocytes. GFLB3 that had been microinjected into Xenopus oocytes was also phosphorylated in Xenopus GV lamina, as judged by Western blotting with C7B8D. Thus, lamin phosphorylation appears to occur prior to oocyte maturation in vivo in both these species. Taken together, our results suggest that the balance between phosphorylation by interphase lamin kinases and dephosphorylation by phosphatases regulates the conformational changes in the lamin B3 N-terminal head domain that in turn regulates the continual in vivo rearrangement and remodeling of the oocyte lamina.

Original languageEnglish
Pages (from-to)501-517
Number of pages17
JournalEuropean Journal of Cell Biology
Volume85
Issue number6
DOIs
Publication statusPublished - Jun 14 2006
Externally publishedYes

Fingerprint

Goldfish
Oocytes
Phosphorylation
Lamins
Xenopus
Nuclear Lamina
Phosphotransferases
Bacteriophage lambda
lamin B2
Proteins
Cell Nucleus Active Transport
Phosphoprotein Phosphatases
Interphase
Nuclear Envelope
Isoelectric Point
Microinjections
Fluorescence Microscopy
Phosphoric Monoester Hydrolases
Confocal Microscopy
Anura

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

Cite this

Phosphorylation of the p34cdc2 target site on goldfish germinal vesicle lamin B3 before oocyte maturation. / Yamaguchi, Akihiko; Katsu, Yoshinao; Matsuyama, Michiya; Yoshikuni, Michiyasu; Nagahama, Yoshitaka.

In: European Journal of Cell Biology, Vol. 85, No. 6, 14.06.2006, p. 501-517.

Research output: Contribution to journalArticle

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