phot1 and phot2 mediate blue light regulation of stomatal opening

Toshinori Kinoshita, Michio Doi, Noriyuki Suetsugu, Takatoshi Kagawa, Masamitsu Wada, Ken Ichiro Shimazaki

Research output: Contribution to journalArticle

576 Citations (Scopus)

Abstract

The stomatal pores of higher plants allow for gaseous exchange into and out of leaves. Situated in the epidermis, they are surrounded by a pair of guard cells which control their opening in response to many environmental stimuli, including blue light1,2. Opening of the pores is mediated by K+ accumulation in guard cells through a K+ channel and driven by an inside-negative electrical potential3. Blue light causes phosphorylation and activation of the plasma membrane H+-ATPase that creates this potential1,2,4-6. Thus far, no blue light receptor mediating stomatal opening has been identified7, although the carotenoid, zeaxanthin, has been proposed. Arabidopsis mutants deficient in specific blue-light-mediated responses have identified7,9-14 four blue light receptors, cryptochrome 1 (cry1), cryptochrome 2 (cry2), phot1 and phot2. Here we show that in a double mutant of phot1 and phot2 stomata do not respond to blue light although single mutants are phenotypically normal. These results demonstrate that phot1 and phot2 act redundantly as blue light receptors mediating stomatal opening.

Original languageEnglish
Pages (from-to)656-660
Number of pages5
JournalNature
Volume414
Issue number6864
DOIs
Publication statusPublished - Dec 6 2001

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Light
Cryptochromes
Proton-Translocating ATPases
Carotenoids
Arabidopsis
Epidermis
Phosphorylation
Cell Membrane

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • General

Cite this

Kinoshita, T., Doi, M., Suetsugu, N., Kagawa, T., Wada, M., & Shimazaki, K. I. (2001). phot1 and phot2 mediate blue light regulation of stomatal opening. Nature, 414(6864), 656-660. https://doi.org/10.1038/414656a

phot1 and phot2 mediate blue light regulation of stomatal opening. / Kinoshita, Toshinori; Doi, Michio; Suetsugu, Noriyuki; Kagawa, Takatoshi; Wada, Masamitsu; Shimazaki, Ken Ichiro.

In: Nature, Vol. 414, No. 6864, 06.12.2001, p. 656-660.

Research output: Contribution to journalArticle

Kinoshita, T, Doi, M, Suetsugu, N, Kagawa, T, Wada, M & Shimazaki, KI 2001, 'phot1 and phot2 mediate blue light regulation of stomatal opening', Nature, vol. 414, no. 6864, pp. 656-660. https://doi.org/10.1038/414656a
Kinoshita T, Doi M, Suetsugu N, Kagawa T, Wada M, Shimazaki KI. phot1 and phot2 mediate blue light regulation of stomatal opening. Nature. 2001 Dec 6;414(6864):656-660. https://doi.org/10.1038/414656a
Kinoshita, Toshinori ; Doi, Michio ; Suetsugu, Noriyuki ; Kagawa, Takatoshi ; Wada, Masamitsu ; Shimazaki, Ken Ichiro. / phot1 and phot2 mediate blue light regulation of stomatal opening. In: Nature. 2001 ; Vol. 414, No. 6864. pp. 656-660.
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