TY - JOUR
T1 - Photo-biohydrogen Production by Photosensitization with Biologically Precipitated Cadmium Sulfide in Hydrogen-Forming Recombinant Escherichia coli
AU - Honda, Yuki
AU - Shinohara, Yuka
AU - Watanabe, Motonori
AU - Ishihara, Tatsumi
AU - Fujii, Hiroshi
N1 - Funding Information:
We thank Dr. Toshihiko Yokota for his support to ICP‐OES analysis and Shohei Nakamura for performing SEM and EDX analyses. This work was partially supported by the grant from the Casio Science Promotion Foundation, the Kurata Grant from the Hitachi Global Foundation, and the grants from JSPS KAKENHI (20K05230).This work was partially supported by the International Institute for Carbon‐Neutral Energy Research (WPI‐I2CNER), which was established by the World Premier International Research Center Initiative (WPI), MEXT, Japan.
Funding Information:
We thank Dr. Toshihiko Yokota for his support to ICP-OES analysis and Shohei Nakamura for performing SEM and EDX analyses. This work was partially supported by the grant from the Casio Science Promotion Foundation, the Kurata Grant from the Hitachi Global Foundation, and the grants from JSPS KAKENHI (20K05230).This work was partially supported by the International Institute for Carbon-Neutral Energy Research (WPI-I2CNER), which was established by the World Premier International Research Center Initiative (WPI), MEXT, Japan.
Publisher Copyright:
© 2020 Wiley-VCH GmbH
PY - 2020/12/1
Y1 - 2020/12/1
N2 - An inorganic-biological hybrid system that integrates features of both stable and efficient semiconductors and selective and efficient enzymes is attractive for facilitating the conversion of solar energy to hydrogen. In this study, we aimed to develop a new photocatalytic hydrogen-production system based on Escherichia coli whole-cell genetically engineered as a biocatalysis for highly active hydrogen formation. The photocatalysis part was obtained by bacterial precipitation of cadmium sulfide (CdS), which is a visible-light-responsive semiconductor. The recombinant E. coli cells were sequentially subjected to CdS precipitation and heterologous [FeFe]-hydrogenase synthesis to yield a CdS@E. coli hybrid capable of light energy conversion and hydrogen formation in a single cell. The CdS@E. coli hybrid achieved photocatalytic hydrogen production with a sacrificial electron donor, thus demonstrating the feasibility of our system and expanding the current knowledge of photosensitization using a whole-cell biocatalyst with a bacterially precipitated semiconductor.
AB - An inorganic-biological hybrid system that integrates features of both stable and efficient semiconductors and selective and efficient enzymes is attractive for facilitating the conversion of solar energy to hydrogen. In this study, we aimed to develop a new photocatalytic hydrogen-production system based on Escherichia coli whole-cell genetically engineered as a biocatalysis for highly active hydrogen formation. The photocatalysis part was obtained by bacterial precipitation of cadmium sulfide (CdS), which is a visible-light-responsive semiconductor. The recombinant E. coli cells were sequentially subjected to CdS precipitation and heterologous [FeFe]-hydrogenase synthesis to yield a CdS@E. coli hybrid capable of light energy conversion and hydrogen formation in a single cell. The CdS@E. coli hybrid achieved photocatalytic hydrogen production with a sacrificial electron donor, thus demonstrating the feasibility of our system and expanding the current knowledge of photosensitization using a whole-cell biocatalyst with a bacterially precipitated semiconductor.
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U2 - 10.1002/cbic.202000383
DO - 10.1002/cbic.202000383
M3 - Article
C2 - 32697401
AN - SCOPUS:85089997311
VL - 21
SP - 3389
EP - 3397
JO - ChemBioChem
JF - ChemBioChem
SN - 1439-4227
IS - 23
ER -