TY - JOUR
T1 - Photo-Degradable Protein-Polymer Hybrid Shells for Caging Living Cells
AU - Yamaguchi, Satoshi
AU - Chujo, Kazuki
AU - Ohashi, Noriyuki
AU - Minamihata, Kosuke
AU - Nagamune, Teruyuki
N1 - Funding Information:
Mr. Kazuho Yamamoto of The University of Tokyo kindly validated the condition for the dead/live staining of cells. This work was supported by the Ministry of Education, Culture, Sports, Science, and Technology (MEXT), Japan, Grant-in-Aid for Young Scientists (A) 24686094 and Challenging Research (Exploratory) 19K22079, and by the Japan Science and Technology Agency (JST), PRESTO 16815021, and MIRAI program 19217334. We thank Rosalie Tran, Ph.D., from Edanz (https://jp.edanz.com/ac) for editing a draft of this manuscript.
Funding Information:
Mr. Kazuho Yamamoto of The University of Tokyo kindly validated the condition for the dead/live staining of cells. This work was supported by the Ministry of Education, Culture, Sports, Science, and Technology (MEXT), Japan, Grant‐in‐Aid for Young Scientists (A) 24686094 and Challenging Research (Exploratory) 19K22079, and by the Japan Science and Technology Agency (JST), PRESTO 16815021, and MIRAI program 19217334. We thank Rosalie Tran, Ph.D., from Edanz ( https://jp.edanz.com/ac ) for editing a draft of this manuscript.
Publisher Copyright:
© 2022 Wiley-VCH GmbH.
PY - 2022/2/24
Y1 - 2022/2/24
N2 - There is growing demand for the precise remote control of cellular functions in various fields. Herein, a method for caging mammalian cells by coating with photodegradable protein-polymer hybrid shells to photo-control their functions without genetic engineering is reported. A layer-by-layer assembly of photocleavable synthetic materials through biotin-streptavidin (SA) binding was employed for cell coating. The cell surfaces were first biotinylated with photocleavable biotinylated poly(ethylene glycol)(PEG)-lipid and then coated by repeatedly layering SA and micelles of the PEG-lipid and photocleavable biotinylated four-arm PEG. The cell extension and adhesion were suppressed with the shells and then triggered with the degradation of the shells by light exposure. Macrophage phagocytosis was also stopped by caging with the shells and restarted by light-guided uncaging. This study provides the first proof of principle that cellular functions can be remotely controlled by steric hinderance of cell surfaces with photodegradable materials.
AB - There is growing demand for the precise remote control of cellular functions in various fields. Herein, a method for caging mammalian cells by coating with photodegradable protein-polymer hybrid shells to photo-control their functions without genetic engineering is reported. A layer-by-layer assembly of photocleavable synthetic materials through biotin-streptavidin (SA) binding was employed for cell coating. The cell surfaces were first biotinylated with photocleavable biotinylated poly(ethylene glycol)(PEG)-lipid and then coated by repeatedly layering SA and micelles of the PEG-lipid and photocleavable biotinylated four-arm PEG. The cell extension and adhesion were suppressed with the shells and then triggered with the degradation of the shells by light exposure. Macrophage phagocytosis was also stopped by caging with the shells and restarted by light-guided uncaging. This study provides the first proof of principle that cellular functions can be remotely controlled by steric hinderance of cell surfaces with photodegradable materials.
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U2 - 10.1002/chem.202103941
DO - 10.1002/chem.202103941
M3 - Article
C2 - 35037703
AN - SCOPUS:85124106900
VL - 28
JO - Chemistry - A European Journal
JF - Chemistry - A European Journal
SN - 0947-6539
IS - 12
M1 - e202103941
ER -