Self-assembling behavior of cationic azobenzene-based lipid (KAON12) was directly examined by transmission electron microscopy and phase-contrast microscopy with small unilamellar vesicles or giant liposomes. In a transfection experimenta using the cationic lipid, UV irradiation improved the transfection efficiency of KAON12 compared without photoirradiation. After KAON12/DNA complex passes through the plasma membrane by endocytosis, at endocytic vehicles, trans to cis photo-isomerization of azobenzene moiety would destabilize the vehicle membrane, thus accelerating membrane fusion of photo-responsive lipid vesicles. This phenomenon aids the escape of plasmid DNA from endocytic vehicles, thus improving transfection efficiency with UV irradiation.
|Number of pages||2|
|Publication status||Published - Dec 1 2006|
|Event||55th Society of Polymer Science Japan Symposium on Macromolecules - Toyama, Japan|
Duration: Sep 20 2006 → Sep 22 2006
|Other||55th Society of Polymer Science Japan Symposium on Macromolecules|
|Period||9/20/06 → 9/22/06|
All Science Journal Classification (ASJC) codes