Photo-enhanced efficiency of cationic lipid-mediated transfection using cationic azobenzene-based lipid

Takeshi Nagasaki; Tsutomu Hamada; Yuko Sato; Kenichi Yoshikawa; Seiji Shinkai

Photo-enhanced efficiency of cationic lipid-mediated transfection using cationic azobenzene-based lipid

Takeshi Nagasaki, Tsutomu Hamada, Yuko Sato, Kenichi Yoshikawa, Seiji Shinkai

Institute for Advanced Study

Research output: Contribution to conference › Paper › peer-review

Abstract

Self-assembling behavior of cationic azobenzene-based lipid (KAON12) was directly examined by transmission electron microscopy and phase-contrast microscopy with small unilamellar vesicles or giant liposomes. In a transfection experimenta using the cationic lipid, UV irradiation improved the transfection efficiency of KAON12 compared without photoirradiation. After KAON12/DNA complex passes through the plasma membrane by endocytosis, at endocytic vehicles, trans to cis photo-isomerization of azobenzene moiety would destabilize the vehicle membrane, thus accelerating membrane fusion of photo-responsive lipid vesicles. This phenomenon aids the escape of plasmid DNA from endocytic vehicles, thus improving transfection efficiency with UV irradiation.

Original language	English
Pages	5229-5230
Number of pages	2
Publication status	Published - Dec 1 2006
Event	55th Society of Polymer Science Japan Symposium on Macromolecules - Toyama, Japan Duration: Sept 20 2006 → Sept 22 2006

Other

Other	55th Society of Polymer Science Japan Symposium on Macromolecules
Country/Territory	Japan
City	Toyama
Period	9/20/06 → 9/22/06

All Science Journal Classification (ASJC) codes

Engineering(all)

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Photo-enhanced efficiency of cationic lipid-mediated transfection using cationic azobenzene-based lipid",

abstract = "Self-assembling behavior of cationic azobenzene-based lipid (KAON12) was directly examined by transmission electron microscopy and phase-contrast microscopy with small unilamellar vesicles or giant liposomes. In a transfection experimenta using the cationic lipid, UV irradiation improved the transfection efficiency of KAON12 compared without photoirradiation. After KAON12/DNA complex passes through the plasma membrane by endocytosis, at endocytic vehicles, trans to cis photo-isomerization of azobenzene moiety would destabilize the vehicle membrane, thus accelerating membrane fusion of photo-responsive lipid vesicles. This phenomenon aids the escape of plasmid DNA from endocytic vehicles, thus improving transfection efficiency with UV irradiation.",

author = "Takeshi Nagasaki and Tsutomu Hamada and Yuko Sato and Kenichi Yoshikawa and Seiji Shinkai",

year = "2006",

month = dec,

day = "1",

language = "English",

pages = "5229--5230",

note = "55th Society of Polymer Science Japan Symposium on Macromolecules ; Conference date: 20-09-2006 Through 22-09-2006",

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TY - CONF

T1 - Photo-enhanced efficiency of cationic lipid-mediated transfection using cationic azobenzene-based lipid

AU - Nagasaki, Takeshi

AU - Hamada, Tsutomu

AU - Sato, Yuko

AU - Yoshikawa, Kenichi

AU - Shinkai, Seiji

PY - 2006/12/1

Y1 - 2006/12/1

N2 - Self-assembling behavior of cationic azobenzene-based lipid (KAON12) was directly examined by transmission electron microscopy and phase-contrast microscopy with small unilamellar vesicles or giant liposomes. In a transfection experimenta using the cationic lipid, UV irradiation improved the transfection efficiency of KAON12 compared without photoirradiation. After KAON12/DNA complex passes through the plasma membrane by endocytosis, at endocytic vehicles, trans to cis photo-isomerization of azobenzene moiety would destabilize the vehicle membrane, thus accelerating membrane fusion of photo-responsive lipid vesicles. This phenomenon aids the escape of plasmid DNA from endocytic vehicles, thus improving transfection efficiency with UV irradiation.

AB - Self-assembling behavior of cationic azobenzene-based lipid (KAON12) was directly examined by transmission electron microscopy and phase-contrast microscopy with small unilamellar vesicles or giant liposomes. In a transfection experimenta using the cationic lipid, UV irradiation improved the transfection efficiency of KAON12 compared without photoirradiation. After KAON12/DNA complex passes through the plasma membrane by endocytosis, at endocytic vehicles, trans to cis photo-isomerization of azobenzene moiety would destabilize the vehicle membrane, thus accelerating membrane fusion of photo-responsive lipid vesicles. This phenomenon aids the escape of plasmid DNA from endocytic vehicles, thus improving transfection efficiency with UV irradiation.

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UR - http://www.scopus.com/inward/citedby.url?scp=33846156581&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:33846156581

SP - 5229

EP - 5230

T2 - 55th Society of Polymer Science Japan Symposium on Macromolecules

Y2 - 20 September 2006 through 22 September 2006

ER -

Photo-enhanced efficiency of cationic lipid-mediated transfection using cationic azobenzene-based lipid

Abstract

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