Photo-enhanced efficiency of cationic lipid-mediated transfection using cationic azobenzene-based lipid

Takeshi Nagasaki, Tsutomu Hamada, Yuko Sato, Kenichi Yoshikawa, Seiji Shinkai

Research output: Contribution to conferencePaper

Abstract

Self-assembling behavior of cationic azobenzene-based lipid (KAON12) was directly examined by transmission electron microscopy and phase-contrast microscopy with small unilamellar vesicles or giant liposomes. In a transfection experimenta using the cationic lipid, UV irradiation improved the transfection efficiency of KAON12 compared without photoirradiation. After KAON12/DNA complex passes through the plasma membrane by endocytosis, at endocytic vehicles, trans to cis photo-isomerization of azobenzene moiety would destabilize the vehicle membrane, thus accelerating membrane fusion of photo-responsive lipid vesicles. This phenomenon aids the escape of plasmid DNA from endocytic vehicles, thus improving transfection efficiency with UV irradiation.

Original languageEnglish
Pages5229-5230
Number of pages2
Publication statusPublished - Dec 1 2006
Event55th Society of Polymer Science Japan Symposium on Macromolecules - Toyama, Japan
Duration: Sep 20 2006Sep 22 2006

Other

Other55th Society of Polymer Science Japan Symposium on Macromolecules
CountryJapan
CityToyama
Period9/20/069/22/06

All Science Journal Classification (ASJC) codes

  • Engineering(all)

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    Nagasaki, T., Hamada, T., Sato, Y., Yoshikawa, K., & Shinkai, S. (2006). Photo-enhanced efficiency of cationic lipid-mediated transfection using cationic azobenzene-based lipid. 5229-5230. Paper presented at 55th Society of Polymer Science Japan Symposium on Macromolecules, Toyama, Japan.