Photo-reactive oligodeoxynucleotide-embedded nanovesicles (PROsomes) with switchable stability for efficient cellular uptake and gene knockdown

Beob Soo Kim; Mitsuru Naito; Rimpei Kamegawa; Hyun Jin Kim; Ryo Iizuka; Takashi Funatsu; Shingo Ueno; Takanori Ichiki; Akihiro Kishimura; Kanjiro Miyata

doi:10.1039/d0cc01750g

Photo-reactive oligodeoxynucleotide-embedded nanovesicles (PROsomes) with switchable stability for efficient cellular uptake and gene knockdown

Beob Soo Kim, Mitsuru Naito, Rimpei Kamegawa, Hyun Jin Kim, Ryo Iizuka, Takashi Funatsu, Shingo Ueno, Takanori Ichiki, Akihiro Kishimura, Kanjiro Miyata

Applied Chemistry

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

A photo-responsive nanovesicle is fabricated by polyion complex (PIC) formation between poly(ethylene glycol) (PEG)-block-polypeptides and photo-reactive oligodeoxynucleotides (PROs)/anti-sense oligonucleotides (ASOs). The ultraviolet (UV) light triggers reversible crosslinking between PROs and ASOs in the vesicular membrane, providing the nanovesicle with switchable stability under physiological conditions. The resulting nanovesicle allows efficient cellular internalization, leading to significant UV-triggered gene knockdown in cultured cells. This journal is

Original language	English
Pages (from-to)	9477-9480
Number of pages	4
Journal	Chemical Communications
Volume	56
Issue number	66
DOIs	https://doi.org/10.1039/d0cc01750g
Publication status	Published - Aug 25 2020

All Science Journal Classification (ASJC) codes

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

Access to Document

10.1039/d0cc01750g

Cite this

@article{a6e446900f8e42f48a72a7fc1c1ca8c6,

title = "Photo-reactive oligodeoxynucleotide-embedded nanovesicles (PROsomes) with switchable stability for efficient cellular uptake and gene knockdown",

abstract = "A photo-responsive nanovesicle is fabricated by polyion complex (PIC) formation between poly(ethylene glycol) (PEG)-block-polypeptides and photo-reactive oligodeoxynucleotides (PROs)/anti-sense oligonucleotides (ASOs). The ultraviolet (UV) light triggers reversible crosslinking between PROs and ASOs in the vesicular membrane, providing the nanovesicle with switchable stability under physiological conditions. The resulting nanovesicle allows efficient cellular internalization, leading to significant UV-triggered gene knockdown in cultured cells. This journal is ",

author = "Kim, {Beob Soo} and Mitsuru Naito and Rimpei Kamegawa and Kim, {Hyun Jin} and Ryo Iizuka and Takashi Funatsu and Shingo Ueno and Takanori Ichiki and Akihiro Kishimura and Kanjiro Miyata",

note = "Funding Information: This research was financially supported by the Center of Innovation (COI) Program (Grant Number: JPMJCE1305) and Research Complex Program from the Japan Science and Technology Agency (JST), Grant-in-Aid for Scientific Research of MEXT of Japan (JSPS KAKENHI Grant Number: 18K19900 for KM), and JSPS Core-to-Core Program (Grant Number: JPJSCCA20170007). We are grateful to Mr H. Hoshi, The University of Tokyo and the Research Hub for Advanced Nano Characterization at The University of Tokyo for the valuable support in TEM observation. Publisher Copyright: {\textcopyright} The Royal Society of Chemistry.",

year = "2020",

month = aug,

day = "25",

doi = "10.1039/d0cc01750g",

language = "English",

volume = "56",

pages = "9477--9480",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "Royal Society of Chemistry",

number = "66",

}

TY - JOUR

T1 - Photo-reactive oligodeoxynucleotide-embedded nanovesicles (PROsomes) with switchable stability for efficient cellular uptake and gene knockdown

AU - Kim, Beob Soo

AU - Naito, Mitsuru

AU - Kamegawa, Rimpei

AU - Kim, Hyun Jin

AU - Iizuka, Ryo

AU - Funatsu, Takashi

AU - Ueno, Shingo

AU - Ichiki, Takanori

AU - Kishimura, Akihiro

AU - Miyata, Kanjiro

N1 - Funding Information: This research was financially supported by the Center of Innovation (COI) Program (Grant Number: JPMJCE1305) and Research Complex Program from the Japan Science and Technology Agency (JST), Grant-in-Aid for Scientific Research of MEXT of Japan (JSPS KAKENHI Grant Number: 18K19900 for KM), and JSPS Core-to-Core Program (Grant Number: JPJSCCA20170007). We are grateful to Mr H. Hoshi, The University of Tokyo and the Research Hub for Advanced Nano Characterization at The University of Tokyo for the valuable support in TEM observation. Publisher Copyright: © The Royal Society of Chemistry.

PY - 2020/8/25

Y1 - 2020/8/25

N2 - A photo-responsive nanovesicle is fabricated by polyion complex (PIC) formation between poly(ethylene glycol) (PEG)-block-polypeptides and photo-reactive oligodeoxynucleotides (PROs)/anti-sense oligonucleotides (ASOs). The ultraviolet (UV) light triggers reversible crosslinking between PROs and ASOs in the vesicular membrane, providing the nanovesicle with switchable stability under physiological conditions. The resulting nanovesicle allows efficient cellular internalization, leading to significant UV-triggered gene knockdown in cultured cells. This journal is

AB - A photo-responsive nanovesicle is fabricated by polyion complex (PIC) formation between poly(ethylene glycol) (PEG)-block-polypeptides and photo-reactive oligodeoxynucleotides (PROs)/anti-sense oligonucleotides (ASOs). The ultraviolet (UV) light triggers reversible crosslinking between PROs and ASOs in the vesicular membrane, providing the nanovesicle with switchable stability under physiological conditions. The resulting nanovesicle allows efficient cellular internalization, leading to significant UV-triggered gene knockdown in cultured cells. This journal is

UR - http://www.scopus.com/inward/record.url?scp=85089712861&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85089712861&partnerID=8YFLogxK

U2 - 10.1039/d0cc01750g

DO - 10.1039/d0cc01750g

M3 - Article

C2 - 32677638

AN - SCOPUS:85089712861

SN - 1359-7345

VL - 56

SP - 9477

EP - 9480

JO - Chemical Communications

JF - Chemical Communications

IS - 66

ER -

Photo-reactive oligodeoxynucleotide-embedded nanovesicles (PROsomes) with switchable stability for efficient cellular uptake and gene knockdown

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this