Photodynamic therapy using indocyanine green loaded on super carbonate apatite as minimally invasive cancer treatment

Koki Tamai, Tsunekazu Mizushima, Xin Wu, Akira Inoue, Minori Ota, Yuhki Yokoyama, Norikatsu Miyoshi, Naotsugu Haraguchi, Hidekazu Takahashi, Junichi Nishimura, Taishi Hata, Chu Matsuda, Yuichiro Doki, Masaki Mori, Hirofumi Yamamoto

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Minimally invasive treatment is getting more and more important in an aging society. The purpose of this study was to explore the possibility of ICG loaded on super carbonate apatite (sCA) nanoparticles as a novel photodynamic therapy (PDT) against cancers. Using colon cancer cells, ICG uptake and anti-tumor effects were examined between the treatments of ICG and sCA-ICG. Reactive oxygen species (ROS) production and temperature rise were also evaluated to explore the underlying mechanism. Atomic force microscopy revealed that the size of sCA-ICG ranged from 10 to 20 nm. In aqueous solution with 0.5% albumin, the temperature increase after laser irradiation was 27.1C and 23.1C in sCA-ICG and ICG, respectively (control DW: 5.7C). A significant increase in ROS generation was noted in cell cultures treated with sCA-ICG plus irradiation compared with those treated with ICG plus irradiation (P < 0.01). Uptake of ICG in the tumor cells significantly increased in sCA-ICG compared with ICG in vitro and in vivo. The fluorescence signals of ICG in the tumor, liver, and kidney faded away in both treatments by 24 hours. Finally, the HT29 tumors treated with sCA-ICG followed by irradiation exhibited drastic tumor growth retardation (P < 0.01), whereas irradiation of tumors after injection of ICG did not inhibit tumor growth. This study shows that sCA is a useful vehicle for ICG-based PDT. Quick withdrawal of ICG from normal organs is unique to sCA-ICG and contrasts with the other nanoparticles remaining in normal organs for a long time.

Original languageEnglish
Pages (from-to)1613-1622
Number of pages10
JournalMolecular Cancer Therapeutics
Volume17
Issue number7
DOIs
Publication statusPublished - Jul 1 2018
Externally publishedYes

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Indocyanine Green
Photochemotherapy
Neoplasms
Therapeutics
Nanoparticles
Reactive Oxygen Species
carboapatite
Temperature
Atomic Force Microscopy
Growth
Colonic Neoplasms
Albumins
Lasers
Cell Culture Techniques
Fluorescence
Kidney
Injections
Liver

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Photodynamic therapy using indocyanine green loaded on super carbonate apatite as minimally invasive cancer treatment. / Tamai, Koki; Mizushima, Tsunekazu; Wu, Xin; Inoue, Akira; Ota, Minori; Yokoyama, Yuhki; Miyoshi, Norikatsu; Haraguchi, Naotsugu; Takahashi, Hidekazu; Nishimura, Junichi; Hata, Taishi; Matsuda, Chu; Doki, Yuichiro; Mori, Masaki; Yamamoto, Hirofumi.

In: Molecular Cancer Therapeutics, Vol. 17, No. 7, 01.07.2018, p. 1613-1622.

Research output: Contribution to journalArticle

Tamai, K, Mizushima, T, Wu, X, Inoue, A, Ota, M, Yokoyama, Y, Miyoshi, N, Haraguchi, N, Takahashi, H, Nishimura, J, Hata, T, Matsuda, C, Doki, Y, Mori, M & Yamamoto, H 2018, 'Photodynamic therapy using indocyanine green loaded on super carbonate apatite as minimally invasive cancer treatment', Molecular Cancer Therapeutics, vol. 17, no. 7, pp. 1613-1622. https://doi.org/10.1158/1535-7163.MCT-17-0788
Tamai, Koki ; Mizushima, Tsunekazu ; Wu, Xin ; Inoue, Akira ; Ota, Minori ; Yokoyama, Yuhki ; Miyoshi, Norikatsu ; Haraguchi, Naotsugu ; Takahashi, Hidekazu ; Nishimura, Junichi ; Hata, Taishi ; Matsuda, Chu ; Doki, Yuichiro ; Mori, Masaki ; Yamamoto, Hirofumi. / Photodynamic therapy using indocyanine green loaded on super carbonate apatite as minimally invasive cancer treatment. In: Molecular Cancer Therapeutics. 2018 ; Vol. 17, No. 7. pp. 1613-1622.
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AU - Yokoyama, Yuhki

AU - Miyoshi, Norikatsu

AU - Haraguchi, Naotsugu

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AU - Nishimura, Junichi

AU - Hata, Taishi

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