Photometric recording of transmembrane potential in outer hair cells

Takashi Nakagawa, John S. Oghalai, Peter Saggau, Richard D. Rabbitt, William E. Brownell

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Cochlear outer hair cells (OHCs) are polarized epithelial cells that have mechanoelectrical transduction channels within their apical stereocilia and produce electromotile force along their lateral wall. Phase shifts, or time delays, in the transmembrane voltage occurring at different axial locations along the cell may contribute to our understanding of how these cells operate at auditory frequencies. We developed a method to optically measure the phase of the OHC transmembrane potential using the voltage-sensitive dye (VSD) di-8-ANEPPS. The exit aperture of a fibre-optic light source was driven in two dimensions so that a 24 μm spot of excitation light could be positioned along the length of the OHC. We used the whole-cell patch-clamp technique in the current-clamp mode to stimulate the OHC at the base. The photometric response and the voltage response were monitored with a photodetector and patch-clamp amplifier, respectively. The photometric response was used to measure the regional changes in the membrane potential in response to maintained (dc) and sinusoidal (ac) current stimuli applied at the base of the cell. We used a neutral density filter to lower the excitation light intensity and reduce phototoxicity. A sensitive detector and lock-in amplifier were used to measure the small ac VSD signal. This permitted measurements of the ac photometric response below the noise floor of the static fluorescence. The amplitude and phase components of the photometric response were recorded for stimuli up to 800 Hz. VSD data at 400-800 Hz show the presence of a small phase delay between the stimulus voltage at the base of the cell and the local membrane potential measured along the lateral wall. Results are consistent with the hypothesis that OHCs exhibit inhomogeneous membrane potentials that vary with position in analogy with the voltage in nerve axons.

Original languageEnglish
Article number001
Pages (from-to)79-86
Number of pages8
JournalJournal of Neural Engineering
Volume3
Issue number2
DOIs
Publication statusPublished - Jun 1 2006

Fingerprint

Outer Auditory Hair Cells
Membrane Potentials
Cells
Electric potential
Coloring Agents
Clamping devices
Light
Dyes
Membranes
Stereocilia
Phototoxic Dermatitis
Patch-Clamp Techniques
Axons
Fluorescence
Epithelial Cells
Cell Membrane
Photodetectors
Phase shift
Fiber optics
Light sources

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Cellular and Molecular Neuroscience

Cite this

Photometric recording of transmembrane potential in outer hair cells. / Nakagawa, Takashi; Oghalai, John S.; Saggau, Peter; Rabbitt, Richard D.; Brownell, William E.

In: Journal of Neural Engineering, Vol. 3, No. 2, 001, 01.06.2006, p. 79-86.

Research output: Contribution to journalArticle

Nakagawa, Takashi ; Oghalai, John S. ; Saggau, Peter ; Rabbitt, Richard D. ; Brownell, William E. / Photometric recording of transmembrane potential in outer hair cells. In: Journal of Neural Engineering. 2006 ; Vol. 3, No. 2. pp. 79-86.
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