Phototropin from Chlamydomonas reinhardtii is functional in Arabidopsis thaliana

Akihiko Onodera, Sam Geun Kong, Michio Doi, Ken Ichiro Shimazaki, John Christie, Nobuyoshi Mochizuki, Akira Nagatani

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Phototropin, a plant blue light photoreceptor, mediates important blue light responses such as phototropism, chloroplast positioning and stomatal opening in higher plants. In Arabidopsis thaliana, two phototoropins, phototropin 1 and 2, are known. Recently, in the unicellular green alga, Chlamydomonas reinhardtii, a phototropin homolog was identified. It exhibits photochemical properties similar to those of higher plant phototropins and is involved in multiple steps of the sexual life cycle of Chlamydomonas. Here, we expressed Chlamydomonas phototropin in Arabidopsis to examine whether it is active in a distantly related plant species. The Arabidopsis mutant deficient in both phototropin 1 and 2 was transformed with a vector containing Chlamydomonas phototropin cDNA fused to a cauliflower mosaic virus 35S promoter. The resulting lines were classified into high, medium and low expressers based on RNA gel blot and immunoblot analyses. Typical phototropin responses were restored in high expression lines. These results demonstrate that Chlamydomonas phototropin is functional in higher plants. Hence, the basic mechanism of phototropin action is highly conserved, even though its apparent physiological functions are quite diverse.

Original languageEnglish
Pages (from-to)367-374
Number of pages8
JournalPlant and Cell Physiology
Volume46
Issue number2
DOIs
Publication statusPublished - Feb 1 2005

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Phototropins
Chlamydomonas reinhardtii
Arabidopsis
Chlamydomonas
Arabidopsis thaliana
blue light
phototropism
Cauliflower mosaic virus
photoreceptors
Chlorophyta
life cycle (organisms)
mechanism of action
Phototropism
chloroplasts
promoter regions
gels
RNA
Caulimovirus
Light
mutants

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science
  • Cell Biology

Cite this

Onodera, A., Kong, S. G., Doi, M., Shimazaki, K. I., Christie, J., Mochizuki, N., & Nagatani, A. (2005). Phototropin from Chlamydomonas reinhardtii is functional in Arabidopsis thaliana. Plant and Cell Physiology, 46(2), 367-374. https://doi.org/10.1093/pcp/pci037

Phototropin from Chlamydomonas reinhardtii is functional in Arabidopsis thaliana. / Onodera, Akihiko; Kong, Sam Geun; Doi, Michio; Shimazaki, Ken Ichiro; Christie, John; Mochizuki, Nobuyoshi; Nagatani, Akira.

In: Plant and Cell Physiology, Vol. 46, No. 2, 01.02.2005, p. 367-374.

Research output: Contribution to journalArticle

Onodera, A, Kong, SG, Doi, M, Shimazaki, KI, Christie, J, Mochizuki, N & Nagatani, A 2005, 'Phototropin from Chlamydomonas reinhardtii is functional in Arabidopsis thaliana', Plant and Cell Physiology, vol. 46, no. 2, pp. 367-374. https://doi.org/10.1093/pcp/pci037
Onodera A, Kong SG, Doi M, Shimazaki KI, Christie J, Mochizuki N et al. Phototropin from Chlamydomonas reinhardtii is functional in Arabidopsis thaliana. Plant and Cell Physiology. 2005 Feb 1;46(2):367-374. https://doi.org/10.1093/pcp/pci037
Onodera, Akihiko ; Kong, Sam Geun ; Doi, Michio ; Shimazaki, Ken Ichiro ; Christie, John ; Mochizuki, Nobuyoshi ; Nagatani, Akira. / Phototropin from Chlamydomonas reinhardtii is functional in Arabidopsis thaliana. In: Plant and Cell Physiology. 2005 ; Vol. 46, No. 2. pp. 367-374.
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