Phylogenetic analysis and Shiga toxin production profiling of Shiga toxin-producing/enterohemorrhagic Escherichia coli clinical isolates

Rui Li, Takaaki Harada, Ken-ichi Honjoh, Takahisa Miyamoto

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Shiga toxin-producing Escherichia coli (STEC) can cause severe illnesses in humans such as hemorrhagic colitis and hemolytic-uremic syndrome. In this study, we carried out genotypic analysis of the Shiga toxin (stx) gene in 120 clinical isolates of STEC and enterohemorrhagic E. coli (EHEC) from patients in a southern district of Japan. We identified 88 stx 1 + and 103 stx 2 + strains. We further identified 12 stx 1 + and stx 2 + isolates expressing little or no Shiga toxin 1 (Stx 1 ) and/or 2 (Stx 2 ) by reversed passive latex agglutination (RPLA) and Vero cell toxicity assays. Among them, 1 strain could not produce Stx 1 , 8 could not produce Stx 2 , and 3 strains could produce neither. Two of the latter three strains were of the non-O157 serotype. Most of the Stx RPLA-negative strains belonged to the stx 1 /stx 2 subtype (11/12, [91.7%]). Our quantitative reverse transcription PCR analysis indicated that the stx genes were not effectively transcribed in the RPLA-negative strains. This is the first report of the isolation of stx-positive strains showing Stx-negative phenotype from stx 1 -bearing strains and non-O157 strains.

Original languageEnglish
Pages (from-to)246-251
Number of pages6
JournalMicrobial Pathogenesis
Volume49
Issue number5
DOIs
Publication statusPublished - Nov 1 2010

Fingerprint

Shiga Toxin 1
Shiga Toxin
Enterohemorrhagic Escherichia coli
Shiga-Toxigenic Escherichia coli
Shiga Toxin 2
Agglutination
Latex
Hemolytic-Uremic Syndrome
Vero Cells
Colitis
Genes
Reverse Transcription
Japan
Phenotype
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Infectious Diseases

Cite this

@article{e735d4ca89d64e289a785efb3b906f37,
title = "Phylogenetic analysis and Shiga toxin production profiling of Shiga toxin-producing/enterohemorrhagic Escherichia coli clinical isolates",
abstract = "Shiga toxin-producing Escherichia coli (STEC) can cause severe illnesses in humans such as hemorrhagic colitis and hemolytic-uremic syndrome. In this study, we carried out genotypic analysis of the Shiga toxin (stx) gene in 120 clinical isolates of STEC and enterohemorrhagic E. coli (EHEC) from patients in a southern district of Japan. We identified 88 stx 1 + and 103 stx 2 + strains. We further identified 12 stx 1 + and stx 2 + isolates expressing little or no Shiga toxin 1 (Stx 1 ) and/or 2 (Stx 2 ) by reversed passive latex agglutination (RPLA) and Vero cell toxicity assays. Among them, 1 strain could not produce Stx 1 , 8 could not produce Stx 2 , and 3 strains could produce neither. Two of the latter three strains were of the non-O157 serotype. Most of the Stx RPLA-negative strains belonged to the stx 1 /stx 2 subtype (11/12, [91.7{\%}]). Our quantitative reverse transcription PCR analysis indicated that the stx genes were not effectively transcribed in the RPLA-negative strains. This is the first report of the isolation of stx-positive strains showing Stx-negative phenotype from stx 1 -bearing strains and non-O157 strains.",
author = "Rui Li and Takaaki Harada and Ken-ichi Honjoh and Takahisa Miyamoto",
year = "2010",
month = "11",
day = "1",
doi = "10.1016/j.micpath.2010.06.005",
language = "English",
volume = "49",
pages = "246--251",
journal = "Microbial Pathogenesis",
issn = "0882-4010",
publisher = "Academic Press Inc.",
number = "5",

}

TY - JOUR

T1 - Phylogenetic analysis and Shiga toxin production profiling of Shiga toxin-producing/enterohemorrhagic Escherichia coli clinical isolates

AU - Li, Rui

AU - Harada, Takaaki

AU - Honjoh, Ken-ichi

AU - Miyamoto, Takahisa

PY - 2010/11/1

Y1 - 2010/11/1

N2 - Shiga toxin-producing Escherichia coli (STEC) can cause severe illnesses in humans such as hemorrhagic colitis and hemolytic-uremic syndrome. In this study, we carried out genotypic analysis of the Shiga toxin (stx) gene in 120 clinical isolates of STEC and enterohemorrhagic E. coli (EHEC) from patients in a southern district of Japan. We identified 88 stx 1 + and 103 stx 2 + strains. We further identified 12 stx 1 + and stx 2 + isolates expressing little or no Shiga toxin 1 (Stx 1 ) and/or 2 (Stx 2 ) by reversed passive latex agglutination (RPLA) and Vero cell toxicity assays. Among them, 1 strain could not produce Stx 1 , 8 could not produce Stx 2 , and 3 strains could produce neither. Two of the latter three strains were of the non-O157 serotype. Most of the Stx RPLA-negative strains belonged to the stx 1 /stx 2 subtype (11/12, [91.7%]). Our quantitative reverse transcription PCR analysis indicated that the stx genes were not effectively transcribed in the RPLA-negative strains. This is the first report of the isolation of stx-positive strains showing Stx-negative phenotype from stx 1 -bearing strains and non-O157 strains.

AB - Shiga toxin-producing Escherichia coli (STEC) can cause severe illnesses in humans such as hemorrhagic colitis and hemolytic-uremic syndrome. In this study, we carried out genotypic analysis of the Shiga toxin (stx) gene in 120 clinical isolates of STEC and enterohemorrhagic E. coli (EHEC) from patients in a southern district of Japan. We identified 88 stx 1 + and 103 stx 2 + strains. We further identified 12 stx 1 + and stx 2 + isolates expressing little or no Shiga toxin 1 (Stx 1 ) and/or 2 (Stx 2 ) by reversed passive latex agglutination (RPLA) and Vero cell toxicity assays. Among them, 1 strain could not produce Stx 1 , 8 could not produce Stx 2 , and 3 strains could produce neither. Two of the latter three strains were of the non-O157 serotype. Most of the Stx RPLA-negative strains belonged to the stx 1 /stx 2 subtype (11/12, [91.7%]). Our quantitative reverse transcription PCR analysis indicated that the stx genes were not effectively transcribed in the RPLA-negative strains. This is the first report of the isolation of stx-positive strains showing Stx-negative phenotype from stx 1 -bearing strains and non-O157 strains.

UR - http://www.scopus.com/inward/record.url?scp=77955424661&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77955424661&partnerID=8YFLogxK

U2 - 10.1016/j.micpath.2010.06.005

DO - 10.1016/j.micpath.2010.06.005

M3 - Article

VL - 49

SP - 246

EP - 251

JO - Microbial Pathogenesis

JF - Microbial Pathogenesis

SN - 0882-4010

IS - 5

ER -