Phylogenetic analysis of enteroaggregative Escherichia coli (eaec) isolates from Japan reveals emergence of ctx-m-14-producing eaec o25:h4 clones related to sequence type 131

Naoko Imuta, Tadasuke Ooka, Kazuko Seto, Ryuji Kawahara, Toyoyasu Koriyama, Tsuyoshi Kojyo, Atsushi Iguchi, Koichi Tokuda, Hideki Kawamura, Kiyotaka Yoshiie, Yoshitoshi Ogura, Tetsuya Hayashi, Junichiro Nishi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Enteroaggregative Escherichia coli (EAEC) causes acute or persistent diarrhea. The aggR gene is widely used as a marker for typical EAEC. The heterogeneity of EAEC is well known; however, there are few reports on the phylogenetic relationships of EAEC. Recently, CTX-M extended-spectrum +-lactamase (ESBL)-producing EAEC strains have been reported worldwide. To characterize EAEC strains in Japan, we investigated the population structure of EAEC. A total of 167 aggR-positive strains isolated from stool specimens from diarrheal patients in Kagoshima (139 strains) and Osaka (28 strains), Japan, between 1992 and 2010 were examined for the prevalence of EAEC virulence markers, the blaCTX-M gene, and the capacity to form biofilms. Multilocus sequence typing was also conducted. EAEC strains were widely distributed across four major E. coli phylogroups. Strains of O111: H21/clonal group 40 (CG40) (30 strains), O126:H27/CG200 (13 strains), and O86a:H27/CG3570 (11 strains) in phylogroup B1 are the historical EAEC clones in Japan, and they exhibited strong biofilm formation. Twenty-nine strains of EAEC O25:H4/ CG131 were identified in phylogroup B2, 79% of which produced CTX-M-14. This clone has emerged since 2003. The clone harbored plasmid-encoded EAEC virulence genes but not chromosomal virulence genes and had lower biofilm-forming capacity than historical EAEC strains. This clone most likely emerged from a pandemic uropathogenic O25:H4/sequence type 131 clone by acquiring an EAEC virulence plasmid from canonical EAEC. Surveillance of the horizontal transfer of both virulence and ESBL genes among E. coli strains is important for preventing a worldwide increase in antimicrobial drug resistance.

Original languageEnglish
Pages (from-to)2128-2134
Number of pages7
JournalJournal of Clinical Microbiology
Volume54
Issue number8
DOIs
Publication statusPublished - Aug 1 2016

Fingerprint

Japan
Clone Cells
Escherichia coli
Virulence
Biofilms
Genes
Plasmids
Multilocus Sequence Typing
Pandemics
Microbial Drug Resistance
Diarrhea

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Cite this

Phylogenetic analysis of enteroaggregative Escherichia coli (eaec) isolates from Japan reveals emergence of ctx-m-14-producing eaec o25:h4 clones related to sequence type 131. / Imuta, Naoko; Ooka, Tadasuke; Seto, Kazuko; Kawahara, Ryuji; Koriyama, Toyoyasu; Kojyo, Tsuyoshi; Iguchi, Atsushi; Tokuda, Koichi; Kawamura, Hideki; Yoshiie, Kiyotaka; Ogura, Yoshitoshi; Hayashi, Tetsuya; Nishi, Junichiro.

In: Journal of Clinical Microbiology, Vol. 54, No. 8, 01.08.2016, p. 2128-2134.

Research output: Contribution to journalArticle

Imuta, Naoko ; Ooka, Tadasuke ; Seto, Kazuko ; Kawahara, Ryuji ; Koriyama, Toyoyasu ; Kojyo, Tsuyoshi ; Iguchi, Atsushi ; Tokuda, Koichi ; Kawamura, Hideki ; Yoshiie, Kiyotaka ; Ogura, Yoshitoshi ; Hayashi, Tetsuya ; Nishi, Junichiro. / Phylogenetic analysis of enteroaggregative Escherichia coli (eaec) isolates from Japan reveals emergence of ctx-m-14-producing eaec o25:h4 clones related to sequence type 131. In: Journal of Clinical Microbiology. 2016 ; Vol. 54, No. 8. pp. 2128-2134.
@article{7fbda714050346fb88ff3da17c3cdf30,
title = "Phylogenetic analysis of enteroaggregative Escherichia coli (eaec) isolates from Japan reveals emergence of ctx-m-14-producing eaec o25:h4 clones related to sequence type 131",
abstract = "Enteroaggregative Escherichia coli (EAEC) causes acute or persistent diarrhea. The aggR gene is widely used as a marker for typical EAEC. The heterogeneity of EAEC is well known; however, there are few reports on the phylogenetic relationships of EAEC. Recently, CTX-M extended-spectrum +-lactamase (ESBL)-producing EAEC strains have been reported worldwide. To characterize EAEC strains in Japan, we investigated the population structure of EAEC. A total of 167 aggR-positive strains isolated from stool specimens from diarrheal patients in Kagoshima (139 strains) and Osaka (28 strains), Japan, between 1992 and 2010 were examined for the prevalence of EAEC virulence markers, the blaCTX-M gene, and the capacity to form biofilms. Multilocus sequence typing was also conducted. EAEC strains were widely distributed across four major E. coli phylogroups. Strains of O111: H21/clonal group 40 (CG40) (30 strains), O126:H27/CG200 (13 strains), and O86a:H27/CG3570 (11 strains) in phylogroup B1 are the historical EAEC clones in Japan, and they exhibited strong biofilm formation. Twenty-nine strains of EAEC O25:H4/ CG131 were identified in phylogroup B2, 79{\%} of which produced CTX-M-14. This clone has emerged since 2003. The clone harbored plasmid-encoded EAEC virulence genes but not chromosomal virulence genes and had lower biofilm-forming capacity than historical EAEC strains. This clone most likely emerged from a pandemic uropathogenic O25:H4/sequence type 131 clone by acquiring an EAEC virulence plasmid from canonical EAEC. Surveillance of the horizontal transfer of both virulence and ESBL genes among E. coli strains is important for preventing a worldwide increase in antimicrobial drug resistance.",
author = "Naoko Imuta and Tadasuke Ooka and Kazuko Seto and Ryuji Kawahara and Toyoyasu Koriyama and Tsuyoshi Kojyo and Atsushi Iguchi and Koichi Tokuda and Hideki Kawamura and Kiyotaka Yoshiie and Yoshitoshi Ogura and Tetsuya Hayashi and Junichiro Nishi",
year = "2016",
month = "8",
day = "1",
doi = "10.1128/JCM.00711-16",
language = "English",
volume = "54",
pages = "2128--2134",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "8",

}

TY - JOUR

T1 - Phylogenetic analysis of enteroaggregative Escherichia coli (eaec) isolates from Japan reveals emergence of ctx-m-14-producing eaec o25:h4 clones related to sequence type 131

AU - Imuta, Naoko

AU - Ooka, Tadasuke

AU - Seto, Kazuko

AU - Kawahara, Ryuji

AU - Koriyama, Toyoyasu

AU - Kojyo, Tsuyoshi

AU - Iguchi, Atsushi

AU - Tokuda, Koichi

AU - Kawamura, Hideki

AU - Yoshiie, Kiyotaka

AU - Ogura, Yoshitoshi

AU - Hayashi, Tetsuya

AU - Nishi, Junichiro

PY - 2016/8/1

Y1 - 2016/8/1

N2 - Enteroaggregative Escherichia coli (EAEC) causes acute or persistent diarrhea. The aggR gene is widely used as a marker for typical EAEC. The heterogeneity of EAEC is well known; however, there are few reports on the phylogenetic relationships of EAEC. Recently, CTX-M extended-spectrum +-lactamase (ESBL)-producing EAEC strains have been reported worldwide. To characterize EAEC strains in Japan, we investigated the population structure of EAEC. A total of 167 aggR-positive strains isolated from stool specimens from diarrheal patients in Kagoshima (139 strains) and Osaka (28 strains), Japan, between 1992 and 2010 were examined for the prevalence of EAEC virulence markers, the blaCTX-M gene, and the capacity to form biofilms. Multilocus sequence typing was also conducted. EAEC strains were widely distributed across four major E. coli phylogroups. Strains of O111: H21/clonal group 40 (CG40) (30 strains), O126:H27/CG200 (13 strains), and O86a:H27/CG3570 (11 strains) in phylogroup B1 are the historical EAEC clones in Japan, and they exhibited strong biofilm formation. Twenty-nine strains of EAEC O25:H4/ CG131 were identified in phylogroup B2, 79% of which produced CTX-M-14. This clone has emerged since 2003. The clone harbored plasmid-encoded EAEC virulence genes but not chromosomal virulence genes and had lower biofilm-forming capacity than historical EAEC strains. This clone most likely emerged from a pandemic uropathogenic O25:H4/sequence type 131 clone by acquiring an EAEC virulence plasmid from canonical EAEC. Surveillance of the horizontal transfer of both virulence and ESBL genes among E. coli strains is important for preventing a worldwide increase in antimicrobial drug resistance.

AB - Enteroaggregative Escherichia coli (EAEC) causes acute or persistent diarrhea. The aggR gene is widely used as a marker for typical EAEC. The heterogeneity of EAEC is well known; however, there are few reports on the phylogenetic relationships of EAEC. Recently, CTX-M extended-spectrum +-lactamase (ESBL)-producing EAEC strains have been reported worldwide. To characterize EAEC strains in Japan, we investigated the population structure of EAEC. A total of 167 aggR-positive strains isolated from stool specimens from diarrheal patients in Kagoshima (139 strains) and Osaka (28 strains), Japan, between 1992 and 2010 were examined for the prevalence of EAEC virulence markers, the blaCTX-M gene, and the capacity to form biofilms. Multilocus sequence typing was also conducted. EAEC strains were widely distributed across four major E. coli phylogroups. Strains of O111: H21/clonal group 40 (CG40) (30 strains), O126:H27/CG200 (13 strains), and O86a:H27/CG3570 (11 strains) in phylogroup B1 are the historical EAEC clones in Japan, and they exhibited strong biofilm formation. Twenty-nine strains of EAEC O25:H4/ CG131 were identified in phylogroup B2, 79% of which produced CTX-M-14. This clone has emerged since 2003. The clone harbored plasmid-encoded EAEC virulence genes but not chromosomal virulence genes and had lower biofilm-forming capacity than historical EAEC strains. This clone most likely emerged from a pandemic uropathogenic O25:H4/sequence type 131 clone by acquiring an EAEC virulence plasmid from canonical EAEC. Surveillance of the horizontal transfer of both virulence and ESBL genes among E. coli strains is important for preventing a worldwide increase in antimicrobial drug resistance.

UR - http://www.scopus.com/inward/record.url?scp=84979523402&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84979523402&partnerID=8YFLogxK

U2 - 10.1128/JCM.00711-16

DO - 10.1128/JCM.00711-16

M3 - Article

C2 - 27252465

AN - SCOPUS:84979523402

VL - 54

SP - 2128

EP - 2134

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 8

ER -