Phylogenetic analysis of manganese-oxidizing fungi isolated from manganese-rich aquatic environments in Hokkaido, Japan

Keishi Takano, Yasoo Itoh, Tagiru Ogino, Kunihiko Kurosawa, Keiko Sasaki

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Three strains of Mn-oxidizing fungi were isolated from manganese-rich aquatic environments: sediment in a stream (Komanoyu) in Mori-machi and inflow to an artificial wetland in Kaminokuni-cho, Hokkaido, Japan. The characteristics of each strain were then established. Genetic analysis based on the ribosomal RNA (rRNA) gene was performed to clarify their classification. The sequences of the 18S rRNA and internal transcribed spacer (ITS1)-5.8S rRNA-ITS2 genes showed that all three strains are Ascomycetes. Based on its morphology, it seems probable that the KY-1 strain from Mori-machi belongs to the genus Phoma or Ampelomyces. The phylogenetic analysis indicates that this strain belongs to Phoma rather than Ampelomyces. Morphological identification of WL-1 and WL-2 strains from Kaminokuni-cho was impossible because of the lack of a sexual stage and specific organs. Phylogenetic analysis of the sequence in the ITS1-5.8S rRNA-ITS2 gene suggests that the WL-1 strain corresponds to Paraconyothyrium sporulosum and that WL-2 also belongs to the genus Paraconiothyrium. Because the ability to oxidize Mn has not been evaluated for most species of Phoma or Paraconiothyrium (Coniothyrium), further study is needed to confirm the status of these three strains.

Original languageEnglish
Pages (from-to)219-223
Number of pages5
JournalLimnology
Volume7
Issue number3
DOIs
Publication statusPublished - Dec 1 2006

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aquatic environment
manganese
RNA
fungus
Japan
phylogenetics
fungi
gene
phylogeny
Phoma
Ampelomyces
genetic analysis
ribosomal RNA
inflow
wetland
Coniothyrium
teleomorphs
sediment
genes
constructed wetlands

All Science Journal Classification (ASJC) codes

  • Ecology
  • Aquatic Science
  • Water Science and Technology

Cite this

Phylogenetic analysis of manganese-oxidizing fungi isolated from manganese-rich aquatic environments in Hokkaido, Japan. / Takano, Keishi; Itoh, Yasoo; Ogino, Tagiru; Kurosawa, Kunihiko; Sasaki, Keiko.

In: Limnology, Vol. 7, No. 3, 01.12.2006, p. 219-223.

Research output: Contribution to journalArticle

Takano, Keishi ; Itoh, Yasoo ; Ogino, Tagiru ; Kurosawa, Kunihiko ; Sasaki, Keiko. / Phylogenetic analysis of manganese-oxidizing fungi isolated from manganese-rich aquatic environments in Hokkaido, Japan. In: Limnology. 2006 ; Vol. 7, No. 3. pp. 219-223.
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