Phylogenetic characterization of Centipeda periodontii, Selenomonas sputigena and Selenomonas species by 16S rRNA gene sequence analysis

S. Sawada, S. Kokeguchi, Fusanori Nishimura, S. Takashiba, Y. Murayama

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The nearly complete 16S rRNA gene sequences for oral Gram-negative anaerobic motile bacteria, Centipeda periodontii, Selenomonas sputigena and Selenomonas species (formerly S. sputigena type strain), were determined in order to unveil their relationship to other oral motile bacteria. To determine the phylogenetic characterization of these bacteria, their 16S rRNA gene sequences were obtained and compared with those from the ribosomal sequence databases previously reported. The 16S rRNA gene sequences of these bacteria were similar to those of Selenomonas ruminantium and Schwartzia succinivorans isolated from rumens, and to Pectinatus cerevisiiphilus isolated from spoiled beer. Among oral bacteria, the nucleotide sequence analysis of these bacteria revealed high nucleotide similarity to Veillonella species, whereas low similarity to oral motile bacteria such as Campylobacter species. Phylogenetic analysis clearly confirmed that C. periodontii and two Selenomonas species were classified as relatives of a group besides Selenomonas, Schwartzia, and Pectinatus species, and not as close relatives to oral motile bacteria, such as Campylobacter species. These results suggest that such oral Gram-negative anaerobic motile bacteria are close relatives of oral bacteria.

Original languageEnglish
Pages (from-to)133-140
Number of pages8
JournalMicrobios
Volume98
Issue number391
Publication statusPublished - Dec 1 1999

Fingerprint

Selenomonas
rRNA Genes
Sequence Analysis
Bacteria
Pectinatus
Gram-Negative Anaerobic Bacteria
Campylobacter
Veillonella
Rumen
Nucleotides
Databases

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)

Cite this

Phylogenetic characterization of Centipeda periodontii, Selenomonas sputigena and Selenomonas species by 16S rRNA gene sequence analysis. / Sawada, S.; Kokeguchi, S.; Nishimura, Fusanori; Takashiba, S.; Murayama, Y.

In: Microbios, Vol. 98, No. 391, 01.12.1999, p. 133-140.

Research output: Contribution to journalArticle

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